- Original investigation
- Open Access
Sitagliptin improved glucose assimilation in detriment of fatty-acid utilization in experimental type-II diabetes: role of GLP-1 isoforms in Glut4 receptor trafficking
© The Author(s) 2018
- Received: 20 September 2017
- Accepted: 12 December 2017
- Published: 11 January 2018
The distribution of glucose and fatty-acid transporters in the heart is crucial for energy consecution and myocardial function. In this sense, the glucagon-like peptide-1 (GLP-1) enhancer, sitagliptin, improves glucose homeostasis but it could also trigger direct cardioprotective actions, including regulation of energy substrate utilization.
Type-II diabetic GK (Goto-Kakizaki), sitagliptin-treated GK (10 mg/kg/day) and wistar rats (n = 10, each) underwent echocardiographic evaluation, and positron emission tomography scanning for [18F]-2-fluoro-2-deoxy-d-glucose (18FDG). Hearts and plasma were isolated for biochemical approaches. Cultured cardiomyocytes were examined for receptor distribution after incretin stimulation in high fatty acid or high glucose media.
Untreated GK rats exhibited hyperglycemia, hyperlipidemia, insulin resistance, and plasma GLP-1 reduction. Moreover, GK myocardium decreased 18FDG assimilation and diastolic dysfunction. However, sitagliptin improved hyperglycemia, insulin resistance, and GLP-1 levels, and additionally, enhanced 18FDG uptake and diastolic function. Sitagliptin also stimulated the sarcolemmal translocation of the glucose transporter-4 (Glut4), in detriment of the fatty acyl translocase (FAT)/CD36. In fact, Glut4 mRNA expression and sarcolemmal translocation were also increased after GLP-1 stimulation in high-fatty acid incubated cardiomyocytes. PI3K/Akt and AMPKα were involved in this response. Intriguingly, the GLP-1 degradation metabolite, GLP-1(9-36), showed similar effects.
Besides of its anti-hyperglycemic effect, sitagliptin-enhanced GLP-1 may ameliorate diastolic dysfunction in type-II diabetes by shifting fatty acid to glucose utilization in the cardiomyocyte, and thus, improving cardiac efficiency and reducing lipolysis.
- Diabetic cardiomyopathy
More than 300 million people worldwide currently suffer from type-II diabetes mellitus (T2DM) . Diabetic cardiomyopathy (DCM) is defined by functional and structural changes at the myocardium, independent of any vascular or cardiac disease . However, DCM and dyslipidemia-associated vasculopathies or hypertension frequently coexist in T2DM subjects, and despite access to a variety of treatments, cardiovascular diseases are the main cause of death in this population . Besides of fatty acids (FA), glucose is a crucial source of energy in the heart, especially after injury. Damaged hearts shift energetic substrate toward highly efficient glucose in detriment of FA, but this metabolic flexibility is impaired under insulin resistance, leaving to FA as unique fuel provider. However, an oversupply of lipids can saturate mitochondria, producing toxic and oxidative metabolites, and cardiac dysfunction . In this regard, cardiac function has been improved described in patients with heart failure after balancing lipid degradation toward glucose oxidation . Also, overexpression of glucose transporter-4 (Glut4) stimulated glucose delivery, reduced FA utilization, and enhanced cardiac performance in obese/T2DM mice . In consonance, deletion of FA-translocase (FAT)/CD36 receptors prevented myocyte triacylglycerol accumulation, increased glucose utilization and ameliorated cardiac dysfunction in PPARα-overexpressed mice resembling T2DM . Thus, regulation of main cellular receptors for glucose and/or FA could represent a promising therapeutic target for DCM.
Sitagliptin, a dipeptidyl peptidase-4 (DPP-4) inhibitor, has demonstrated insulin dependent and independent cardio-protective actions in DCM by increasing glucagon-like peptide-1 (GLP-1), and following activation of, at least, its pancreatic and cardiac receptors (GLP-1R) [8, 9]. In pancreas, GLP-1R activation stimulated insulin secretion, whereas in the cardiovascular system, it promoted anti-fibrotic/-hypertrophic/-apoptotic responses [10, 11]. However, the effects of sitagliptin on cardiac energetic substrate uptake, in particular, on Glut4 and FAT/CD36 distribution have not been elucidated. In addition, previous data suggest that the main degradation peptide of GLP-1, GLP-1(9-36), may also interact with GLP-1R or different receptors to induce cardio-salutary actions [10, 12]. Therefore, both GLP-1 and GLP-1(9-36) could trigger direct insulin-mimetic effects on cardiomyocytes and enhance cardiac alterations associated to T2DM.
Experimental model of T2DM
A polygenic non-obese non-hypertensive model of T2DM was used in this work. Goto-Kakizaki (GK) rats exhibit similar metabolic, hormonal and vascular disorders that the human T2DM, offering a convenient model for the study of T2DM per se, without the confounding effects of obesity or hypertension. Male GK (Taconic, Denmark) were kept on artificial 12-h light–dark cycles (7 a.m.–7 p.m.) at 25 °C. Animals had free access to chow and water. Once T2DM became well-established (at the 25th week), rats were daily treated (at 10 a.m.) with sitagliptin [Merck Sharp & Dohme (Spain), 10 mg/Kg/day] or vehicle by a gavage, as previously described . Sex and aged-matched wistar rats were also examined (N = 10, each group). Body weight, diet consumption and systolic blood pressure (measured by tail-cuff method) were weekly monitored. After 20 weeks of sitagliptin-administration, plasma (collected from cava vein) and hearts were isolated (at 3–7 p.m.) under 1.5% isoflurane-O2 anaesthesia. Plasma glucose, lipid profile, hepatic and renal parameters were enzymatically measured in the clinical department of the Hospital. Hearts were rinsed, dried, and weighted. After atria excision, left ventricles were frozen in liquid-N2 for biochemical assays. These investigations adhered to the Guide for the Care and Use of Laboratory Animals (NIH Publication No. 85–23, revised 1996), and the Ethics Committee of the IIS-Fundación Jiménez Díaz Hospital granted approval for these experiments.
Glucose homeostasis and OGTT
Small-animal PET imaging
Cardiac structure and function
Mouse C2C12 myoblasts (ATCC, USA) were kindly given by Dr. Konhilas (University of Arizona, USA), and maintained in DMEM supplemented with 9% foetal calf serum, 5 mM d-glucose, 50 U/ml penicillin, and 50 μg/ml streptomycin. Before confluency, the medium was replaced to differentiation medium containing DMEM and 2% horse serum. After 4 additional days, the differentiated C2C12 cells fused into myotubes. Then, cells were switched to serum-free quiescent medium overnight before stimulation. The hyperlipidemic or hyperglycemic conditions were mimicked by 6–12 h incubation with high concentrations of a common saturated free FA (FFA) [Na+-palmitate (16:0), 0.12 mM], or glucose (d-glucose, 25 mM), respectively (Sigma). These concentrations were not lethal after 12 h incubation . Palmitate was previously conjugated with BSA in a 3:1 molar ratio as published earlier . In control cells, BSA was added as described but in the absence of palmitate. Some cells were pre-treated with GLP-1 (1 nM) or GLP-1(9-36) (0.3 nM) (Sigma) 30 min before stimulation. Wortmannin (50 nM) was added 1 h before stimulation.
Particulate fractions and Western Blot (WB)
To quantify total cellular content of specific proteins (FABP3, PPARα, SPTLC2 and PDK4), a piece (~ 50 mg) of homogenized LV (Bullet Bender, Next Advance) was dissolved in ice-cold protein lysis buffer A (50 mM Tris–HCl pH 7.5, 1 mM EDTA, 2% SDS + 1/250 mammalian protease inhibitors). To evaluate the glucose- and FA-transporters distribution, a piece (~ 50 mg) of homogenized LV was suspended in ice-cold lysis buffer B (2 mM EDTA, 2 mM phenyl-methyl-sulfonyl fluoride, and 1 µM pepstatin A in PBS). Cytosolic proteins were collected in supernatant after cold centrifugation at 50,000g for 30 min. The pellet, with sarcolemmal proteins was suspended in ice-cold lysis buffer B. In vitro, 50 μg of cell extracts from cultured cardiomyocytes were homogenized in ice-cold buffer C containing 1 mM EDTA, 250 mM sucrose, and 10 mM Tris, pH 7.5. The homogenates were cold-centrifuged (5 min at 760g), and supernatants were isolated and centrifuged (1 h at 31,000g) to pellet the sarcolemma enriched-fraction. Then, the second supernatant was subjected to centrifugation (1 h at 190,000g) to pellet the endosome enriched-fraction.
Equal amounts of all protein extracts were loaded and separated on polyacrylamide gels, transferred to membranes (iBlot, Thermo Fisher), and probed with specific primary antibodies anti-FABP3, -GPAT1, -SPTLC2 (Aviva System Biology), -PDK4, -PPARα (Sigma Aldrich), -Glut4, -Glut1, -FAT/CD36, -phospho-AKT(Ser473), -phospho-AMPKα1(Ser496) or -phospho-IRS1(Ser307) (Thermo Fisher). Anti-GAPDH or anti-pan-cadherin (Sigma) were used as loading control for cytosol or sarcolemma, respectively. Then, secondary antibodies (GE Healthcare) were used for chemo-luminescence development. A representative gel of the rats or at least three independent experiments with the semi-quantification scores (n-fold) are shown.
Total RNA was extracted from homogenized ventricle (~ 50 mg) or cultured cardiomyocytes by dissolving in Trizol reagent (Thermo Fisher). Equal amounts of RNA were reverse-transcripted to obtain the cDNA for multiplex QPCR, as previously described . The gene expression assays were Glut4 (Mn00436615_m1), FAT/CD36 (Rn00580728_m1), acyl-CoA dehydrogenase long chain (ACADl) (Rn00562121_m1), acyl-CoA dehydrogenase medium chain (ACADm) (Rn00566390_m1) and CPT1b (Rn00682395_m1) Fam-fluorophores. The housekeeping gene was eukaryotic ribosomic 18s Vic-fluorophore (4310893E). Amplification conditions were: 2′ at 50 °C, 10′′ at 95 °C and 40 cycles of 15′′ at 95 °C and 1′ at 60 °C. All samples were prepared in triplicate to obtain their threshold cycle (Ct). If deviation for each triplicate were higher than 0.3 cycles, Ct was not considered. The relative expression for each gene was achieved following the model R = 2−ΔΔCt. We show the average quantification (-fold gene vs. 18s) of two QPCRs of all rats or three independent cultured cardiomyocytes assays.
Data are expressed as mean ± standard deviation. Multiple comparisons were performed by non-parametric Kruskal–Wallis test followed by a Mann–Whitney test. Statistical significance was defined from p < 0.05.
Sitagliptin attenuated hyperglycemia and insulin resistance in GK rats
Sitagliptin improved glucose uptake and diastolic dysfunction in the GK myocardium
Next, a defect in glucose uptake in T2DM hearts could originate cardiac dysfunction . Thus, by 2D-Echo-Doppler we evaluated cardiac dimensions and function in GK rats (Fig. 2b). GK exhibited a significant increase of the LV posterior wall (LVPW) thickness, and a reduction of LV diastolic and systolic diameters (LVDD and LVSD, respectively). Accordingly, LV mass was elevated in GK myocardia. As expected , GK rats preserved the ejection fraction, but they prolonged the deceleration time and diminished the E/A ratio, suggesting diastolic dysfunction (Fig. 2b). Interestingly, sitagliptin attenuated these hypertrophic parameters, and lessen diastolic dysfunction.
Sitagliptin stimulated Glut4 translocation in detriment of FAT/CD36 in GK hearts
Since glucose and FA metabolism could be tightly coupled and inversely regulated , we next analysed the levels of the most relevant FA transporter in the myocardium. FAT/CD36 also synthetizes in the cytosol and moves away from endosome vesicles to the sarcolemma for FA uptake . In this model, we detected FAT/CD36 mainly in cytosolic fractions of wistar rats, but it was partially shifted to sarcolemmal location in GK (Fig. 3e, left). In parallel, FAT/CD36 mRNA (Fig. 3f), but not its cytosolic isoform (Fig. 3e, left), was increased. Interestingly, sitagliptin attenuated sarcolemmal FAT/CD36 translocation (Fig. 3e, right), suggesting a potential counterbalance with stimulated Glut4 transporters.
GLP-1 isoforms improved Glut4 translocation in HF-stimulated cardiomyocytes
Consequently, in order to explain the endosomal increase of Glut4 after GLP-1 and GLP-1(9-36) incretins in HF milieu, we quantified Glut4 mRNA expression by QPCR in the cardiomyocytes. Glut4 transcripts were initially triggered by HF, but progressively decreased up to 12 h (Fig. 4d, left). Importantly, both GLP-1 and GLP-1(9-36) recovered Glut4 mRNA levels after 12 h of HF incubation (Fig. 4d, right).
Sitagliptin reduced FA utilization in GK myocardia
Finally, the expression of a crucial interconnecting enzyme between FA and glucose metabolic pathways was studied. Pyruvate dehydrogenase kinase-4 (PDK4), a PPARα-dependent inhibitory enzyme of the glycolytic pyruvate dehydrogenase complex , was triggered in GK myocardia but restored after sitagliptin administration (Fig. 5d). This data enforces the potential cardiac anti-lipolysis action of sitagliptin by restoring the balance toward glucose utilization in GK rats.
According to the Framingham Heart Study, the risk of heart failure in diabetes is increased 2.4-fold in men and fivefold in women compared to non-diabetic subjects . In this study, we observed that non-hypertensive non-obese T2DM (GK) rats exhibited hyperglycemia and hyperlipidemia, inefficient production of insulin and GLP-1, and also cardiac dysfunction. Importantly, this systemic insulin resistance may be mirrored at the myocardium. By PET, GK decreased cardiac glucose assimilation which was paralleled to a reduction in sarcolemmal content of Glut4 and Akt/AMPKα activation, while FAT/CD36 was elevated. Similar data were observed in T1DM  and obese-T2DM [22–27] animals. These data suggest that heart may suffer from insulin resistance in obesity and T2DM, and FA could be preferred as energetic substrate in these conditions. In this regard, metabolic mediators of FA utilization may be upregulated. We observed that cytosolic and mitochondrial FA-transporters (FABP3 and CPT1b), and FAO enzymes (ACADl and ACADm) were over-expressed in GK myocardia. Moreover, a related transcription factor such as PPARα, and a glycolytic inhibitor such as PDK4, were also stimulated. Thus, under glucose deficit and insulin resistance, most FA could be used for FAO and energy consecution in the heart. In this sense, we did not observe stimulation of GPAT-1 and SPTLC2 as rate-limiting enzymes of DAG and ceramide formation, respectively. However, excessive FAO could lead to ROS accumulation and mitochondrial uncoupling, becoming a maladaptive response that rise lipotoxicity and reduce energy efficiency . In fact, our GK rats revealed cardiac hypertrophy and diastolic dysfunction.
Unfortunately, there is not an efficient and specific treatment for DCM. Intensive glycemic goals have failed to prevent cardiac complications in long-term diabetic patients or have even increased cardiovascular mortality . New therapeutic strategies capable of preserving heart function while contributing to the overall care of diabetes may be required. In this line, a correction of the metabolic imbalance has led to positive outcomes. A reduction of cardiac FAO and/or stimulation of glucose oxidation improved heart failure, ischaemic injury, and DCM in mice and patients [29, 30]. In this work, sitagliptin, a DPP-4 inhibitor that prevents GLP-1 degradation into GLP-1(9-36) and other metabolites, reduced hyperglycemia and insulin resistance, and increased GLP-1 levels in fasting and non-fasting states, as previously described [8, 31]. More importantly, sitagliptin stimulated cardiac glucose assimilation and enriched Glut4 (but not Glut1) at sarcolemma locations, in detriment of FAT/CD36. Similarly, Vyas et al. described that exenatide, an agonist of GLP-1R, increased total Glut4 whereas Glut1 was unchanged . Although Glut1 could compensate Glut4 lessening at least in hypertensive and hypertrophied hearts [33, 34], Glut1 is constitutively present on plasma membranes of multiple tissues including myocardium . In consequence, FA utilization might be also reduced after sitagliptin administration. Indeed, the expression of FABP3, CPT1b, and ACADl and ACADm, was diminished in GK-treated hearts. Also, PPARα and PDK4, were lessened. Thus, sitagliptin-stabilized GLP-1 may improve glucose assimilation, and co-ordinately, decline excessive FA utilization and subsequent potential mitochondrial saturation and ineffectiveness. By balancing metabolic and oxidative state, diabetic hearts could enhance their structure and performance . In fact, GK-treated rats exhibited an improvement of cardiac hypertrophy and diastolic dysfunction. However, despite evidence from clinical studies have demonstrated that sitagliptin could exert cardioprotection after specific heart injury (i.e., ischemia) , this inhibitor could not reduced LV diastolic dysfunction and lipid profile in T2DM subjects . Likely, both the presence of different DPP-4-targets than GLP-1 and the absence of GLP-1 degradative molecules [i.e., GLP-1(9-36), GLP-1(28-36)] may affect cardiovascular pathophysiology in diabetes. In this sense, DPP4 could also act as a direct mediator of endothelial dysfunction, via PAR2 activation and prostanoids release .
Interestingly, GLP-1 actions can be partially driven in an insulin-independent and myocardial specific fashion . GLP-1R has been found in extra-pancreatic tissue like heart, and thus, GLP-1 could increase myocardial glucose uptake independently of its ability to enhance insulin secretion . Also, sitagliptin stimulated myocardial 18FDG uptake in non-diabetic patients with dilated cardiomyopathy, without altering glucose homeostasis . We now demonstrate that GLP-1 ameliorated HF-associated insulin resistance in cultured cardiomyocytes by up-regulation of both sarcolemmal and endosome Glut4 isoforms. GLP-1 also overexpressed Glut4 mRNA expression after 12 h HF-incubation. However, GLP-1 did not alleviate Glut4 levels after HG incubation, possibly because HG can also bind Glut4 and might induce a negative feed-back regulation by itself  or by its deviation products (i.e., hexosamines) . Thus, we hypothesize that GLP-1 may directly promote both Glut4 translocation and mRNA expression after HF in the myocardium, as previously seen in insulin resistant muscle and liver, via PI3K/Akt and/or AMPK activation [43, 44]. In fact, sitagliptin-treated hearts exhibited a moderate increase of phospho-Akt476 and phospho-AMPKα496 in parallel to Glut4 translocation. Accordingly, the enrichment of sarcolemmal Glut4 induced by GLP-1 in HF-incubated cardiomyocytes, was attenuated by a specific PI3K inhibitor. This data confirms the participation of the axes Akt/PI3K and AMPKα, likely activated by GLP-1R stimulation [42, 44].
However, some GLP-1 activities may be also ascribed to GLP-1(9-36). This non-insulinotropic peptide is present in the circulation at higher levels than GLP-1 , though it shows 100-fold lower affinity for pancreatic GLP-1R [46, 47]. Remarkably, GLP-1(9-36) exhibited similar effects to GLP-1 as anti-fibrotic/apoptotic factor on cardiomyocytes , and reduced cardiac injury in ischemia/reperfusion models by GLP-1R dependent or independent mechanisms . Also, GLP-1(9-36) induced vasodilatation through nitric oxide formation , and protected against oxidation in cardiac and vascular cells [48–50]. Here we show that GLP-1(9-36) improved cardiomyocyte Glut4 expression and sarcolemmal translocation after HF, in a similar way than GLP-1. GLP-1(9-36) may also activate GLP-1R and downstream Akt/PI3K and AMPKα mediators. Thus, the sequential modification of GLP-1 by DPP-4 from an insulinotropic to an insulinomimetic hormone [i.e., GLP-1(9-36)] could be also beneficial for cardiovascular protection . These data also supports the insulin-independent actions of incretins and support the major outcomes of GLP-1R agonists over DPP-4 inhibitors . In addition, GLP-1(9-36) may yield to a variety of N-terminal cleavage products, such as GLP-1(28-36), with demonstrated anti-oxidative proprieties .
Limitation of the study
The evaluation of FA assimilation by PET at the myocardia could have added important information to our quantification of FA-transporters and enzymes. However, due to the extremely short half-life (~ 20 min) of a labelled FFA (i.e., [11C]-FFA) and the distance to the centre of synthesis, we could not achieve this approach. Also, despite we confirmed a weight-neutral effect of sitagliptin, it also reduced the food intake by 13% (p < 0.05), which may have contributed to the results.
Since cardiac dysfunction is dramatically increasing in T2DM patients, new insulinomimetic-based interventions with cardioprotective proprieties are required. In this regard, sitagliptin improved hyperglycemia and insulin resistance in GK rats, and also, it enhanced cardiac glucose assimilation in detriment of excessive FA utilization and likely lipotoxicity. The sitagliptin-stabilized incretin, GLP-1, could inversely regulate sarcolemmal location of Glut4 and FAT/CD36 in cardiomyocytes via PI3K/Akt and AMPKα, but similar actions could be also triggered by GLP-1(9-36) metabolite. Therefore, these effects may occur independent of insulin regulation, opening the therapeutic utility to other cardiomyopathies different than DCM.
OL and MAM designed, discussed and wrote the work. ER, BP and AG-B performed the experimental model and biochemical procedures. MO and FC completed the PET approach. AC-V, JT and JE provided critical analysis. All authors read and approved the final manuscript.
The authors declare that they have no competing interests.
Availability of data and materials
Consent for publication
Ethics approval and consent to participate
This work was supported by national grants from Ministerio de Educación y Ciencia (SAF2009-08367), Comunidad de Madrid (CCG10-UAM/BIO-5289), and PIE13/00051 and PI14/00386 (IS. Carlos III). Merck Sharp and Dohme (Darmstadt, Germany) provided sitagliptin and partial financial support to the conduct of the study.
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
- Mathers CD, Loncar D. Projections of global mortality and burden of disease from 2002 to 2030. PLoS Med. 2006;3:e442.View ArticlePubMedPubMed CentralGoogle Scholar
- Goyal BR, Mehta AA. Diabetic cardiomyopathy: pathophysiological mechanisms and cardiac dysfuntion. Hum Exp Toxicol. 2013;32:571–90.View ArticlePubMedGoogle Scholar
- Fuentes-Antrás J, Picatoste B, Ramírez E, Egido J, Tuñón J, Lorenzo Ó. Targeting metabolic disturbance in the diabetic heart. Cardiovasc Diabetol. 2015;14:173.View ArticleGoogle Scholar
- Zhou YT, Grayburn P, Karim A, Shimabukuro M, Higa M, Baetens D, et al. Lipotoxic heart disease in obese rats: implications for human obesity. Proc Natl Acad Sci USA. 2000;97:1784–9.View ArticlePubMedPubMed CentralGoogle Scholar
- Wallhaus TR, Taylor M, DeGrado TR, Russell DC, Stanko P, Nickles RJ, et al. Myocardial free fatty acid and glucose use after carvedilol treatment in patients with congestive heart failure. Circulation. 2001;103:2441–6.View ArticlePubMedGoogle Scholar
- Belke DD, Larsen TS, Gibbs EM, Severson DL. Glucose metabolism in perfused mouse hearts overexpressing human GLUT-4 glucose transporter. Am J Physiol Endocrinol Metab. 2001;280:E420–7.View ArticlePubMedGoogle Scholar
- Habets DDJ, Coumans WA, Voshol PJ, den Boer MAM, Febbraio M, Bonen A, et al. AMPK-mediated increase in myocardial long-chain fatty acid uptake critically depends on sarcolemmal CD36. Biochem Biophys Res Commun. 2007;355:204–10.View ArticlePubMedGoogle Scholar
- Green JB, Bethel MA, Paul SK, Ring A, Kaufman KD, Shapiro DR, et al. Rationale, design, and organization of a randomized, controlled Trial Evaluating Cardiovascular Outcomes with Sitagliptin (TECOS) in patients with type 2 diabetes and established cardiovascular disease. Am Heart J. 2013;166(983–989):e7.Google Scholar
- Noyan-Ashraf MH, Momen MA, Ban K, Sadi A-M, Zhou Y-Q, Riazi AM, et al. GLP-1R agonist liraglutide activates cytoprotective pathways and improves outcomes after experimental myocardial infarction in mice. Diabetes. 2009;58:975–83.View ArticlePubMedPubMed CentralGoogle Scholar
- Picatoste B, Ramírez E, Caro-Vadillo A, Iborra C, Egido J, Tuñón J, et al. Sitagliptin reduces cardiac apoptosis, hypertrophy and fibrosis primarily by insulin-dependent mechanisms in experimental type-II diabetes. potential roles of GLP-1 isoforms. PLoS ONE. 2013;8:e78330.View ArticlePubMedPubMed CentralGoogle Scholar
- Ravassa S, Zudaire A, Carr RD, Díez J. Antiapoptotic effects of GLP-1 in murine HL-1 cardiomyocytes. Am J Physiol Heart Circ Physiol. 2011;300:H1361–72.View ArticlePubMedGoogle Scholar
- Ban K, Noyan-Ashraf MH, Hoefer J, Bolz S-S, Drucker DJ, Husain M. Cardioprotective and vasodilatory actions of glucagon-like peptide 1 receptor are mediated through both glucagon-like peptide 1 receptor-dependent and -independent pathways. Circulation. 2008;117:2340–50.View ArticlePubMedGoogle Scholar
- Orskov C, Holst JJ. Radio-immunoassays for glucagon-like peptides 1 and 2 (GLP-1 and GLP-2). Scand J Clin Lab Invest. 1987;47:165–74.View ArticlePubMedGoogle Scholar
- Li R, Yang J, Yang Y, Ma N, Jiang B, Sun Q, et al. Speckle tracking echocardiography in the diagnosis of early left ventricular systolic dysfunction in type II diabetic mice. BMC Cardiovasc Disord. 2014;14:141.View ArticlePubMedPubMed CentralGoogle Scholar
- Ramirez E, Klett-Mingo M, Ares-Carrasco S, Picatoste B, Ferrarini A, Ruperez FJ, et al. Eplerenone attenuated cardiac steatosis, apoptosis and diastolic dysfunction in experimental type-II diabetes. Cardiovasc Diabetol. 2013;12:172.View ArticlePubMedPubMed CentralGoogle Scholar
- Stanley WC, Recchia FA, Lopaschuk GD. Myocardial substrate metabolism in the normal and failing heart. Physiol Rev. 2005;85:1093–129.View ArticlePubMedGoogle Scholar
- Schwenk RW, Luiken JJFP, Bonen A, Glatz JFC. Regulation of sarcolemmal glucose and fatty acid transporters in cardiac disease. Cardiovasc Res. 2008;79:249–58.View ArticlePubMedGoogle Scholar
- Lopaschuk GD, Ussher JR, Folmes CDL, Jaswal JS, Stanley WC. Myocardial fatty acid metabolism in health and disease. Physiol Rev. 2010;90:207–58.View ArticlePubMedGoogle Scholar
- Madrazo JA, Kelly DP. The PPAR trio: regulators of myocardial energy metabolism in health and disease. J Mol Cell Cardiol. 2008;44:968–75.View ArticlePubMedGoogle Scholar
- Kannel WB, Hjortland M, Castelli WP. Role of diabetes in congestive heart failure: the Framingham study. Am J Cardiol. 1974;34:29–34.View ArticlePubMedGoogle Scholar
- Eguchi M, Kim YH, Kang KW, Shim CY, Jang Y, Dorval T, et al. Ischemia-reperfusion injury leads to distinct temporal cardiac remodeling in normal versus diabetic mice. PLoS ONE. 2012;7:e30450.View ArticlePubMedPubMed CentralGoogle Scholar
- van den Brom CE, Huisman MC, Vlasblom R, Boontje NM, Duijst S, Lubberink M, et al. Altered myocardial substrate metabolism is associated with myocardial dysfunction in early diabetic cardiomyopathy in rats: studies using positron emission tomography. Cardiovasc Diabetol. 2009;8:39.View ArticlePubMedPubMed CentralGoogle Scholar
- Welch MJ, Lewis JS, Kim J, Sharp TL, Dence CS, Gropler RJ, et al. Assessment of myocardial metabolism in diabetic rats using small-animal PET: a feasibility study. J Nucl Med. 2006;47:689–97.PubMedGoogle Scholar
- Shoghi KI, Gropler RJ, Sharp T, Herrero P, Fettig N, Su Y, et al. Time course of alterations in myocardial glucose utilization in the Zucker diabetic fatty rat with correlation to gene expression of glucose transporters: a small-animal PET investigation. J Nucl Med. 2008;49:1320–7.View ArticlePubMedPubMed CentralGoogle Scholar
- Guiducci L, Liistro T, Burchielli S, Panetta D, Bonora D, Di Cecco P, et al. Contribution of organ blood flow, intrinsic tissue clearance and glycaemia to the regulation of glucose use in obese and type 2 diabetic rats: a PET study. Nutr Metab Cardiovasc Dis. 2011;21:726–32.View ArticlePubMedGoogle Scholar
- Battiprolu PK, Hojayev B, Jiang N, Wang ZV, Luo X, Iglewski M, et al. Metabolic stress-induced activation of FoxO1 triggers diabetic cardiomyopathy in mice. J Clin Invest. 2012;122:1109–18.View ArticlePubMedPubMed CentralGoogle Scholar
- Yue P, Arai T, Terashima M, Sheikh AY, Cao F, Charo D, et al. Magnetic resonance imaging of progressive cardiomyopathic changes in the db/db mouse. Am J Physiol Heart Circ Physiol. 2007;292:H2106–18.View ArticlePubMedGoogle Scholar
- Mazzone T. Intensive glucose lowering and cardiovascular disease prevention in diabetes: reconciling the recent clinical trial data. Circulation. 2010;122:2201–11.View ArticlePubMedPubMed CentralGoogle Scholar
- Fillmore N, Mori J, Lopaschuk GD. Mitochondrial fatty acid oxidation alterations in heart failure, ischaemic heart disease and diabetic cardiomyopathy. Br J Pharmacol. 2014;171:2080–90.View ArticlePubMedPubMed CentralGoogle Scholar
- Witteles RM, Keu KV, Quon A, Tavana H, Fowler MB. Dipeptidyl peptidase 4 inhibition increases myocardial glucose uptake in nonischemic cardiomyopathy. J Card Fail. 2012;18:804–9.View ArticlePubMedGoogle Scholar
- Ishibashi Y, Matsui T, Takeuchi M, Yamagishi S. Sitagliptin augments protective effects of GLP-1 against advanced glycation end product receptor axis in endothelial cells. Horm Metab Res. 2011;43:731–4.View ArticlePubMedGoogle Scholar
- Vyas AK, Yang K-C, Woo D, Tzekov A, Kovacs A, Jay PY, et al. Exenatide improves glucose homeostasis and prolongs survival in a murine model of dilated cardiomyopathy. PLoS ONE. 2011;6. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3040766/. Accessed 11 Apr 2017.
- Linz D, Hohl M, Mahfoud F, Reil J-C, Linz W, Hübschle T, et al. Cardiac remodeling and myocardial dysfunction in obese spontaneously hypertensive rats. J Transl Med. 2012;10:187.View ArticlePubMedPubMed CentralGoogle Scholar
- Stenbit AE, Katz EB, Chatham JC, Geenen DL, Factor SM, Weiss RG, et al. Preservation of glucose metabolism in hypertrophic GLUT4-null hearts. Am J Physiol Heart Circ Physiol. 2000;279:H313–8.View ArticlePubMedGoogle Scholar
- Morissette MR, Howes AL, Zhang T, Heller Brown J. Upregulation of GLUT1 expression is necessary for hypertrophy and survival of neonatal rat cardiomyocytes. J Mol Cell Cardiol. 2003;35:1217–27.View ArticlePubMedGoogle Scholar
- Doenst T, Nguyen TD, Abel ED. Cardiac metabolism in heart failure—implications beyond ATP production. Circ Res. 2013;113:709–24.View ArticlePubMedPubMed CentralGoogle Scholar
- McCormick LM, Kydd AC, Read PA, Ring LS, Bond SJ, Hoole SP, et al. Chronic dipeptidyl peptidase-4 inhibition with sitagliptin is associated with sustained protection against ischemic left ventricular dysfunction in a pilot study of patients with type 2 diabetes mellitus and coronary artery disease clinical perspective. Circ Cardiovasc Imaging. 2014;7:274–81.View ArticlePubMedGoogle Scholar
- Oe H, Nakamura K, Kihara H, Shimada K, Fukuda S, Takagi T, et al. Comparison of effects of sitagliptin and voglibose on left ventricular diastolic dysfunction in patients with type 2 diabetes: results of the 3D trial. Cardiovasc Diabetol. 2015;14:83.View ArticlePubMedPubMed CentralGoogle Scholar
- Romacho T, Vallejo S, Villalobos LA, Wronkowitz N, Indrakusuma I, Sell H, et al. Soluble dipeptidyl peptidase-4 induces microvascular endothelial dysfunction through proteinase-activated receptor-2 and thromboxane A2 release. J Hypertens. 2016;34:869–76.View ArticlePubMedGoogle Scholar
- Moreno P, Nuche-Berenguer B, Gutiérrez-Rojas I, Acitores A, Sancho V, Valverde I, et al. Normalizing action of exendin-4 and GLP-1 in the glucose metabolism of extrapancreatic tissues in insulin-resistant and type 2 diabetic states. J Mol Endocrinol. 2012;48:37–47.View ArticlePubMedGoogle Scholar
- Huang S, Czech MP. The GLUT4 glucose transporter. Cell Metab. 2007;5:237–52.View ArticlePubMedGoogle Scholar
- Ebeling P, Koistinen HA, Koivisto VA. Insulin-independent glucose transport regulates insulin sensitivity. FEBS Lett. 1998;436:301–3.View ArticlePubMedGoogle Scholar
- Schwenk RW, Angin Y, Steinbusch LKM, Dirkx E, Hoebers N, Coumans WA, et al. Overexpression of vesicle-associated membrane protein (VAMP) 3, but not VAMP2, protects glucose transporter (GLUT) 4 protein translocation in an in vitro model of cardiac insulin resistance. J Biol Chem. 2012;287:37530–9.View ArticlePubMedPubMed CentralGoogle Scholar
- Armoni M, Harel C, Karnieli E. Transcriptional regulation of the GLUT4 gene: from PPAR-gamma and FOXO1 to FFA and inflammation. Trends Endocrinol Metab. 2007;18:100–7.View ArticlePubMedGoogle Scholar
- Egan JM, Meneilly GS, Habener JF, Elahi D. Glucagon-like peptide-1 augments insulin-mediated glucose uptake in the obese state. J Clin Endocrinol Metab. 2002;87:3768–73.View ArticlePubMedGoogle Scholar
- Elahi D, Egan JM, Shannon RP, Meneilly GS, Khatri A, Habener JF, et al. GLP-1 (9-36) amide, cleavage product of GLP-1 (7-36) amide, is a glucoregulatory peptide. Obesity (Silver Spring). 2008;16:1501–9.View ArticleGoogle Scholar
- Tomas E, Stanojevic V, Habener JF. GLP-1 (9-36) amide metabolite suppression of glucose production in isolated mouse hepatocytes. Horm Metab Res. 2010;42:657–62.View ArticlePubMedGoogle Scholar
- Anagnostis P, Athyros VG, Adamidou F, Panagiotou A, Kita M, Karagiannis A, et al. Glucagon-like peptide-1-based therapies and cardiovascular disease: looking beyond glycaemic control. Diabetes Obes Metab. 2011;13:302–12.View ArticlePubMedGoogle Scholar
- Mima A, Hiraoka-Yamomoto J, Li Q, Kitada M, Li C, Geraldes P, et al. Protective effects of GLP-1 on glomerular endothelium and its inhibition by PKCβ activation in diabetes. Diabetes. 2012;61:2967–79.View ArticlePubMedPubMed CentralGoogle Scholar
- Read PA, Khan FZ, Heck PM, Hoole SP, Dutka DP. DPP-4 inhibition by sitagliptin improves the myocardial response to dobutamine stress and mitigates stunning in a pilot study of patients with coronary artery disease. Circ Cardiovasc Imaging. 2010;3:195–201.View ArticlePubMedGoogle Scholar
- Tahrani AA, Barnett AH, Bailey CJ. Pharmacology and therapeutic implications of current drugs for type 2 diabetes mellitus. Nat Rev Endocrinol. 2016;12:566–92.View ArticlePubMedGoogle Scholar