Fig. 4

GLP-1 and GLP-1(9-36) increased endosomal and sarcolemmal Glut4 in hyperlipidemic cardiomyocytes. Glut4 expression in endosome- (e) and sarcolemmal (s)-enriched fractions of a HG- and b HF-stimulated C2C12. Some cells were pre-treated with GLP-1 or GLP-1(9-36). *p < 0.05 vs. endosome. ^†p < 0.05 and ^∂p < 0.05 vs. sarcolemmal. ^§p < 0.05 vs. endosome from HF-incubated cells. c Sarcolemmal (left) and endosome (right) Glut4 expression was detected in Wortmannin pre-incubated GLP-1-stimulated cardiomyocytes, under HF medium. *p < 0.05 vs. HF, ^†p < 0.05 vs. HF + GLP-1. d Left, Glut4 mRNA expression after HF incubation (1–12 h). Right, Glut4 mRNA expression after 12 h HF stimulation and/or GLP-1 or GLP-1(9-36). *p < 0.05 and **p < 0.01 vs. control. ^†p < 0.05 vs. HF-incubated cells