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Exenatide can inhibit calcification of human VSMCs through the NF-kappaB/RANKL signaling pathway

Cardiovascular Diabetology volume 13, Article number: 153 (2014) Cite this article

Abstract

Background

Arterial calcification is an important pathological change of diabetic vascular complication. Osteoblastic differentiation of vascular smooth muscle cells (VSMCs) plays an important cytopathologic role in arterial calcification. The glucagon-like peptide-1 receptor agonists (GLP-1RA), a novel type of antidiabetic drugs, exert cardioprotective effects through the GLP-1 receptor (GLP-1R). However, the question of whether or not GLP-1RA regulates osteoblastic differentiation and calcification of VSMCs has not been answered, and the associated molecular mechanisms have not been examined.

Methods

Calcifying VSMCs (CVSMCs) were isolated from cultured human arterial smooth muscle cells through limiting dilution and cloning. The extent of matrix mineralization was measured by Alizarin Red S staining. Protein expression and phosphorylation were detected by Western blot. Gene expression of receptor activator of nuclear factor-κB ligand (RANKL) was silenced by small interference RNA (siRNA).

Results

Exenatide, an agonist of GLP-1 receptor, attenuated β-glycerol phosphate (β-GP) induced osteoblastic differentiation and calcification of human CVSMCs in a dose- and time-dependent manner. RANKL siRNA also inhibited osteoblastic differentiation and calcification. Exenatide decreased the expression of RANKL in a dose-dependent manner. 1,25 vitD3 (an activator of RANKL) upregulated, whereas BAY11-7082 (an inhibitor of NF-κB) downregulated RANKL, alkaline phosphatase (ALP), osteocalcin (OC), and core binding factor α1 (Runx2) protein levels and reduced mineralization in human CVSMCs. Exenatide decreased p-NF-κB and increased p-AMPKα levels in human CVSMCs 48 h after treatment. Significant decrease in p-NF-κB (p-Ser276, p-Ser536) level was observed in cells treated with exenatide or exenatide++κBAY11-7082.

Conclusion

GLP-1RA exenatide can inhibit human VSMCs calcification through NF-κB/RANKL signaling.

Introduction

Diabetes mellitus is a major risk factor for the development of cardiovascular disease [1]. Currently, treatment of diabetes and its cardiovascular complications with classic drugs is effective in less than 50% of patients [2]. Novel therapies are therefore required to manage diabetes mellitus and mitigate cardiovascular risks. GLP-1 is an incretin hormone that is rapidly released from intestinal L-cells after food intake. GLP-1 has insulinotropic effects, and it lowers blood glucose in a glucose-dependent manner [3]. Targeting of glucagon-like peptide-1 receptor (GLP-1R) has been developed to complement conventional treatment options for diabetes mellitus. This class of drugs is currently undergoing clinical trials for the treatment of type 2 diabetes.

Exenatide is a synthetic peptide sharing 53% sequence homology with GLP-1 and has been used as an agonist of mammalian GLP-1 receptor (GLP-1RA) for the treatment of type 2 diabetes [4],[5]. In clinical trials, treatment with exenatide once a week assisted more patients in reaching the majority of ADA-recommended therapeutic goals than treatments with other drugs, such as sitagliptin, pioglitazone, or insulin glargine [6]. However, the residual adverse effect of sulphonylurea was also higher in patients who were treated with exenatide once a week [7]. Compared to conventional treatments, GLP-1RAs not only have an advantage in lowering blood sugar, but also have other benefits. For example, it has been reported that exenatide confers cardioprotection [4],[5],[8]. Arterial calcification is an important pathological change of diabetic vascular complication, which results in cardiovascular events and amputations [9]. However, the question of whether or not GLP-1RA regulates arterial calcification has not been answered, and the associated molecular mechanisms have not been examined.

Studies have suggested that arterial calcification is an active and tightly regulated process similar to bone formation [10],[11]. The differentiation of vascular smooth muscle cell (VSMC) to an osteoblastic phenotype is a key component of the cytopathologic foundation of arterial calcification [12]-[15]. Calcifying VSMCs (CVSMCs) can spontaneously form calcification nodules and express osteoblast phenotype genes, such as ALP, OC, and Runx2 [16],[17], which are often used together as a cell model in arterial calcification research [18]. Receptor activator of nuclear factor-κB ligand (RANKL) is a member of the tumor necrosis factor family and is important for bone remodeling [19]. Researchers have discoved that OPG-deficient mice develop severe osteoporosis and arterial calcification [20],[21]. OPG inhibits nuclear kappa B (NF-κB) by binding RANKL. RANKL directly promotes calcification of VSMCs [22]. In addition, elevated serum OPG has been observed to be associated with vascular calcification in humans with T2DM [23]. The elevated serum OPG may interfere with RANK/RANKL interactions in the vascular wall. In cardiovascular diseases, RANKL is positively associated with circulating OPG [24].

In the present study, we investigated the effects of exenatide and RANKL in the osteoblastic differentiation and calcification of CVSMCs and examined the associated mechanisms.

Materials and methods

Cell culture and in vitro calcification

Human VSMCs were isolated from excess donor vasculature after kidney transplantations as previously described [25]. Briefly, the arterial tissues were removed and rinsed several times in Hanks’s balanced salt solution. The arterial tissues were then minced and digested in 5 ml of digestion solution (0.25 mg/ml soybean trypsin inhibitor, 0.125 mg/ml elastase, 2.0 mg/ml crystallized bovine albumin, 10 mg/ml collagenase I, and 15 mM HEPES) at 37°C for 45 min. The digested cells were filtered through a sterile 100-mM nylon mesh, centrifuged at 1,000 rpm for 10 min, and washed with Dulbecco’s modified Eagle’s medium (DMEM) containing high glucose concentration (4500 mg/L) and 10% FBS (Gibco-BRL). CVSMCs were isolated from cultured VSMCs when multicellular nodules spontaneously appeared. Briefly, cells were cloned by limiting dilution. Colonial cells were identified as CVSMCs by positive α-actin staining and by high ALP expression and formation of calcified nodules. This study was approved by the Ethics Review Board of Second Xiangya Hospital, Central South University. Written informed consent was obtained from patients.

Mineralization assay

The extent of matrix mineralization in cultured CVSMCs was determined by Alizarin Red S staining. Briefly, cells were fixed with 4% formaldehyde for 10 min at room temperature and exposed to 2% Alizarin Red S for 5 min. Cells were then washed with PBS to remove excess dye. For quantitative analysis of Alizarin Red S staining, the dye was released from the cell matrix by incubating with cetyl-pyridinium chloride for 15 min. The amount of released dye was quantified by spectrophotometry at 540 nm. Results were normalized to the total amount of cellular proteins.

Osteoblastic differentiation

Human CVSMCs were fixed in 4% paraformaldehyde, washed with Tris-Tween-20 buffer, and permeabilized for 20 min in buffer containing 0.1% triton-X. After blocking for 1 h, cells were incubated with primary antibodies overnight at 4°C, followed by secondary antibodies for 30 min. anti-ALP, anti-Runx2, and anti-OC primary antibodies and secondary antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA).

To analyze the dose dependence of calcification on exenatide, CVSMCs were cultured with β-GP (10 mM) and 0, 100 pM, 1 nM, and 100 nM of exenatide in serum-free high glucose DMEM for 48 hrs. ALP activity was determined using the spectrophotometric measurement of p-nitrophenol level in the culture medium [26]. Osteocalcin level was measured using a radioimmunoassay kit (DiaSorin, Stillwater, MN, USA). The ALP activity and OC level were then normalized to the total amount of cellular protein.

Silencing of RANKL by RNA interference

Three RANKL target sequences (S1: 5’-GTGCAGAAATGGCGAGAATAC-3’, S2: 5’-GGAUGGCUCAUGGUUAGAUTT-3’, S3: 5’-CGGAUCAGGAUGCAACAUATT-3’) were used to design and synthesize siRNA. A scrambled siRNA sequence was used as a control for siRNA transfection as previously described [27]. CVSMCs were plated onto 60-mm dishes and cultured in DMEM without antibiotics 24 hours before siRNA transformation. Cells were transfected with RANKL siRNA or control siRNA using Lipofectamine 2000 (Invitrogen, Grand Island, NY) according to the instructions of the manufacturer and continually cultured for 72 hours. Cells were then harvested for analyses of RANKL expression. Among the 3 siRNAs, siRNA derived from the S2 target sequence was the most effective in silencing RANKL expression and was used for further experiments in this study.

Western blot

Protein concentration was measured using a BCA Protein Assay kit (Beyotime, Shanghai, China). 20-μg of total protein was loaded onto a 12% SDS-PAGE gel and transferred onto nitrocellulose membranes. After blocking with 5% non-fat milk for 1 h, membranes were incubated with primary antibody overnight at 4°C and subsequently incubated with HRP-labeled secondary antibody (1:2000 dilution) for 2 h at room temperature. Reactive proteins were detected using chemiluminescent reagents (Pierce, Rockford, IL, USA). To control for loading efficiency, the blots were stripped and reprobed with GAPDH (glyceraldehyde-3-phosphatedehydrogenase) antibody. Primary antibodies for RANKL, AMPKα, Phospho-AMPKα Thr172, ERK1/2, Phospho-ERK, JNK, Phospho-JNK, NF-κB p65, phospho-NF-κB (p-NF-κB Ser276, p-NF-κB Ser529 and p-NF-κB Ser536), and GAPDH were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA).

Statistical analysis

Data were presented as mean ± standard deviation (SD) and analyzed using Statistical Product and Service Solutions (SPSS, version 17.0). Differences between two groups were analyzed using Student’s t-test. P <0.05 was considered statistically significant.

Results

Effects of exenatide and RANKL-siRNA on osteoblastic differentiation and calcification of human CVSMCs

To determine the effect of GLP-1RA and RANKL on the osteoblastic differentiation and calcification of human CVSMCs, a GLP-1 receptor agonist (exenatide) and RANKL siRNA were used. Figure 1A shows that RNA interference efficiently knocked down RANKL gene expression in human CVSMCs. Treatment with siRNA-RANKL blocked the expression of RANKL protein in human CVSMCs. Further examination found that exenatide inhibited ALP activity (Figure 1B) and OC secretion (Figure 1C) in β-GP-treated VSMCs in a dose-dependent manner. Two nM of exenatide was selected for further studies. Two nM of exenatide downregulated ALP protein level in human CVSMCs compared to cells without exenatide treatment (Figure 1D and E). Moreover, treatment of human CVSMCs with exenatidemean + RANKL-siRNA or RANKL-siRNA alone downregulated ALP and RANKL protein levels (Figure 1D and E). Alizarin Red S staining showed weaker staining in CVSMCs treated with exenatidemean + RANKL-siRNA compared to control cells or cells treated with exenatide or RANKL-siRNA alone (Figure 1 F and G). These results indicated that exenatide treatment and RANKL siRNA attenuated the osteoblastic differentiation and calcification of human CVSMCs.

Figure 1
figure1

Exenatide and RANKL-siRNA attenuate osteoblastic differentiation and calcification of human CVSMCs. A) Representative Western blot of RANKL protein levels in human CVSMCs transfected with control siRNA (C-siRNA) or R-siRNA for 48 hours. B) Exenatide inhibited ALP activities in β-GP-treated VSMCs in a dose-dependent manner. VSMCs were treated for 48 hrs. N = 5, **P <0.001 vs. control (0 concentration of exenatide). C) Exenatide inhibited OC levels in β-GP-treated VSMCs in a dose-dependent manner. VSMCs were treated for 48 hrs. N = 5, **P <0.001 vs. 0 concentration of exenatide. D) Representative Western blot of ALP and RANKL protein expressions. Human CVSMCs were treated with or without exenatide (2 nM) and RANKL-siRNA (R-siRNA) for 48 h. GAPDH was used as the loading control. E) Semi-quantitative analysis of bands in Western blot at 48 hrs in each group. Bars represent meanmean ± SD. #P < 0.001 between two indicated groups. N = 5. F) Representative Alizarin Red S staining. Human CVSMCs were treated with various agents for 15 days. Magnificationmean ± 200. G) Quantification of Alizarin red S staining. The dye was extracted and quantified as described in the Method section. Bars represent meanmean ± SD. #P < 0.001 between two indicated groups. N = 5.

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Dose-effect relationship of the regulation of RANKL expression by exenatide in human CVSMCs and possible signaling pathways

Recent studies have demonstrated that several signaling pathways regulate RANKL expression in osteoblasts or VSMCs, such as AMPK, JNK, ERK1/2 and NF-κB signaling [11],[28],[29]. As shown in Figure 2A, exenatide decreased the expression of RANKL in a dose-dependent manner. Also, exenatide decreased p-NF-κB and increased p-AMPKα levels in human CVSMCs 48 h after treatment. In contrast, exenatide did not affect the protein level of AMPKα, ERK1/2, p-ERK1/2, JNK, p-JNK, or NF-κB (Figure 2).

Figure 2
figure2

Exenatide-inhibited osteoblastic differentiation and calcification of human CVSMCs requires NF-κB. A) Human CVSMCs were treated with different concentrations of exenatide for 48 hrs. Representative Western blot of RANKL, AMPKα, p-AMPKα, ERK1/2, p- ERK1/2, JNK, p-JNK, NF-κB, p-NF-κB protein expression. B) Semi-quantitative analysis of bands in Western blot. GAPDH was used as the loading control. Bars represent meanmean ± SD. *P < 0.01 and #P < 0.001 between two indicated groups. N = 5.

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Effects of NF-κB inhibitor and RANKL activator on exenatide-inhibited osteoblastic differentiation and calcification of human CVSMCs

Human CVSMCs were treated with or without exenatide (2 nM), BAY11-7082 (an inhibitor of NF-κB, 10 μM), and 1,25-dihydroxyvitamin D3 (1,25vitD3) (an activator of RANKL, 10-7 M) [30] for 48 h. The expression of ALP, OC, and Runx2 proteins was detected by Western blot. Results showed that 2 nM of exenatide downregulated ALP, OC, and Runx2 protein levels in human CVSMCs compared to cells without exenatide treatment (Figure 3A and 3B). 1,25vitD3 upregulated ALP, OC, and Runx2 protein levels in human CVSMCs compared to cells treated with exenatide alone. Treatment of human CVSMCs with 2 nM of exenatide and 10 μM of BAY11-7082 more effectively downregulated ALP, OC, and Runx2 protein levels compared to cells treated with 2 nM of exenatide, 1,25 vitD3, or cells without treatment. Weaker Alizarin Red S staining was observed in CVSMCs cultured with exenatide alone than in cells cultured without exenatide. Stronger Alizarin Red S staining was observed in human CVSMCs cultured with exenatide and 1,25 vitD3 than in cells cultured with exenatide alone or exenatide +κBAY11-7082 (Figure 3C and D). These results suggested that exenatide treatment may attenuate osteoblastic differentiation and calcification of human CVSMCs through NF-κB signaling. RANKL activation exhibits the opposite effect.

Figure 3
figure3

Exenatide-inhibited osteoblastic differentiation and calcification of human CVSMCs can be regulated by NF-κB inhibitor and RANKL activator. A, B) Human CVSMCs were treated with exenatide (2 nM), 1,25 VitD3 (10-7 M), and BAY11-7082 (10 μM) for 48 h. ALP, OC and Runx2 protein expressions were detected by Western blot. GAPDH was used as the loading control Bars represent meanmean ± SD. #P < 0.001 between two indicated groups. N = 5. C) Human CVSMCs were treated with various agents for 15 days. The calcification was visualized by Alizarin Red S staining. Magnificationmean ± 200. D) Quantification of Alizarin red S staining. The dye was extracted and quantified as described in the Method section. Bars represent meanmean ± SD. #P < 0.001 between two indicated groups. N = 5.

Full size image

Roles of RANKL and phosphorylation of NF-κB in exenatide-inhibited osteoblastic differentiation and calcification of human CVSMCs

We further investigated RANKL, NF-κB and p-NF-κB (p-Ser529, p-Ser276, p-Ser536) protein levels in human CVSMCs treated with or without exenatide (2 nM), BAY11-7082 (10 μM), and 1,25 vitD3 (10-7 M) (Figure 4A). The protein level of RANKL was significantly decreased in cells treated with exenatide alone compared to cells without exenatide treatment. Pretreatment of cells with the NF-κB inhibitor BAY11-7082 downregulated the protein level of RANKL. Conversely, pretreatment of cells with the RANKL activator 1,25 vitD3 upregulated the protein level of RANKL. These results indicated that RANKL is a downstream mediator for exenatide-reduced osteoblastic differentiation and calcification of CVSMCs. Western blot showed a significant decrease in p-NF-κB (p-Ser276, p-Ser536) level in cells treated with exenatide or exenatide +κBAY11-7082 compared to cells in the no treatment group (Figure 4A and 4B). No changes in total NF-κB or NF-κB p65 (p-Ser529) levels were observed in human CVSMCs with different treatments. These results indicated that suppression of NF-κB phosphorylation, but not its expression, is necessary for exenatide-inhibited osteoblastic differentiation and calcification in human CVSMCs.

Figure 4
figure4

The effect of exenatide in the phosphorylation of NF-κB and the expression of RANKL in human CVSMCs. A) Human CVSMCs were treated with exenatide (2 nM),1,25VitD3(10-7 M) and BAY11-7082(10 μM) for 48 h. RANKL, NF-κB, p-NF-κB (S529, S276, S536) levels were detected by Western blot. GAPDH was used as the loading control. B) These results were reported at 48 h in each group. Bars represent meanmean ± SD. *P < 0.01 and #P < 0.001 between two indicated groups. N = 5.

Full size image

Discussion

Type 2 diabetes mellitus is a progressive disease characterized by persistent insulin resistance and declining pancreatic β-cell function [31]. However, long-term glycemic control is hard to achieve because of deteriorating β-cell function. A multicentre randomized parallel-group trial suggested that exenatide was more effective in improving β-cell function compared to insulin and piogltazone; hence, early initiation of β-cell-protective therapy with exenatide may halt the decline in β-cell function in type 2 diabetes [32]. Indeed, two years after treatment initiation, patients on exenatide twice a day showed a significant reduction in body mass index and hemoglobin A1c compared with patients treated with a long-acting insulin analog [33]. In addition, even after only three months, exenatide therapy exhibited similar effects on microvascular endothelial function, inflammation markers, oxidative stress, and vascular activation as metformin in patients with obesity and pre-diabetes [34]. In this study, exenatide was able to inhibit human VSMCs calcification in cells cultured with high glucose concentration, suggesting that exenatide may exert its effect dependent on glucose concentration. Therefore, the detected alterations may play a role in the prevention of diabetes-induced vascular calcification.

Recent studies have suggested that GLP-1RA exhibits potential cardioprotective properties in addition to its role in lowering glucose level [35]. In animal models, exenatide has been demonstrated to be capable of protecting the heart against ischemia/reperfusion injury [4],[36], reducing infarct size, improving cardiac function [4], and retarding atherosclerotic lesion formation [37]. Bao et al study demonstrated that sustained GLP-1 receptor activation plays an important role in providing cardioprotection in the setting of acute myocardial I/R injury [38]. However, the question of whether or not GLP-1RA protects the cardiovascular system by regulating VSMCs osteoblastic differentiation and calcification has not been answered, and the associated molecular mechanism is still unclear. Expression of ALP, OC, and Runx2 is a marker of osteoblastic differentiation, while matrix mineralization is a hallmark of the osteoblast phenotype [14],[15]. In this study, we demonstrated that exenatide downregulated the expression of ALP, OC, and Runx2. In addition, exenatide inhibited mineralized nodule formation. We show for the first time that the GLP-1RA exenatide can inhibit osteoblastic differentiation and calcification of human CVSMCs.

Overexpression of RANKL has been found to stimulate VSMC mineralization [19]-[22],[39]. However, most of the evidence for the direct role of RANKL signaling in arterial calcification is derived from animal studies. Little data is available on the role of RANKL signaling in relation to arterial calcification in human cells. The present study showed that exenatide inhibited RANKL expression in human CVSMCs through the NF-κB pathway and mitigated CVSMCs calcification. These findings suggest that exenatide inhibits the calcification of human CVSMCs via the NF-κB/RANKL signaling pathway.

The RANKL complex, consisting of the membrane-bound RANK, its ligand RANKL, and the decoy receptor OPG, is thought to be important for bone metabolism. Osteoblasts express OPG and RANKL, which regulate osteoclast differentiation through the activation of RANK [40]. The present study showed that exenatide could decrease the expression of RANKL and inhibit the expression of ALP, OC, and Runx2 in human CVSMCs. Furthermore, inhibition of RANKL expression by siRNA abrogated the role of exenatide in ALP expression in human CVSMCs. These findings suggest that RANKL is a key mediator in exenatide-induced inhibition of osteoblastic differentiation of human CVSMCs.

The NF-κB, MAPK, ERK, and NFAT signaling pathways have been demonstrated to regulate RANKL expression in osteoblast cells [29],[41] and VSMCs [11],[28]. In this study, exenatide inhibited the activation of NF-κB in human CVSMCs. Pretreatment of cells with NF-κB inhibitor inhibited the expression of RANKL in human CVSMCs. The NF-κB inhibitor BAY11-7082 abolished the effect of RANKL activator (1,25 vitD3) - promoted RANKL expression. In contrast, no changes in AMPKα, ERK1/2, p-ERK1/2, JNK, and p-JNK protein level have been observed in this study.

The NF-κB family consists of five members, RelA (p65), RelB, NF-κB1 (p50), NF-κB2 (p52), and c-Rel. p65 heterodimer is the predominant form in most cell types. The activation of NF-κB p65 is mediated by phosphorylation at Ser529, Ser536 and Ser276 residues [42]. However, the different functions of phosphorylation at these residues in arterial calcification have not been identified. In this study, we revealed that blocking NF-κB p65 phosphorylation at Ser536 and Ser276, but not at Ser529, abolishes NF-κB p65 activity. These findings provide direct evidence that phosphorylation of NF-κB p65 at Ser536 and Ser276 is required for β-GP-induced NF-κB activation in the osteoblastic differentiation of human CVSMCs.

Conclusion

In conclusion, GLP-1RA exenatide can inhibit osteoblastic differentiation and calcification of human CVSMCs through the NF-κB/RANKL signaling pathway. This finding may represent a new therapeutic potential in arterial calcification for GLP-1RA, which is currently undergoing clinical trials for the treatment of type 2 diabetes.

Authors’ contributions

JKZ, PT, YJW, and YW carried out the molecular and cellular studies. JKZ and PT participated in the drafted the manuscript. JYH, ZYT, and WH participated in the graph preparation and the statistical analysis. YSL conceived of the study, and participated in its design and coordination and helped to draft the manuscript. All authors read and approved the final manuscript.

Abbreviations

β-GP:

β-glycerol phosphate

ALP:

Alkaline phosphatase

OC:

Osteocalcin

Runx2:

Core binding factor α1

VSMCs:

Vascular smooth muscle cells

CVSMCs:

Calcifying vascular smooth muscle cells

NF-κB:

Nuclear Factor κB

RANKL:

Receptor activator of nuclear factor-κB ligand

GLP-1RA:

Glucagon-like peptide-1 receptor agonists

GLP-1R:

Glucagon-like peptide-1 receptor

CVSMCs:

Calcifying vascular smooth muscle cells

SPSS:

Statistical package for the social science

siRNA:

Small interference RNA

MAPK:

Mitogen-activated protein kinase

AMPK:

AMP-activated protein kinase

JNK:

c-Jun N-terminal kinase

OPG:

Osteoprotegerin

ERK:

Extracellular signal-regulated kinase

RANK:

Nuclear factor κB receptor activator

NEAT:

Nuclear factor of activated T-cells

References

  1. 1.

    Sarwar N, Gao P, Seshasai SR, Gobin R, Kaptoge S, Di Angelantonio E, Ingelsson E, Lawlor DA, Selvin E, Stampfer M, Stehouwer CD, Lewington S, Pennells L, Thompson A, Sattar N, White IR, Ray KK, Danesh J: Diabetes mellitus, fasting blood glucose concentration, and risk of vascular disease: a collaborative meta-analysis of 102 prospective studies. Lancet. 2010, 375: 2215-2222. 10.1016/S0140-6736(10)60484-9.

    CAS  Article  PubMed  Google Scholar 

  2. 2.

    Saydah SH, Fradkin J, Cowie CC: Poor control of risk factors for vascular disease among adults with previously diagnosed diabetes. JAMA. 2004, 291: 335-342. 10.1001/jama.291.3.335.

    CAS  Article  PubMed  Google Scholar 

  3. 3.

    Lee YS, Jun HS: Anti-diabetic actions of glucagon-like peptide-1 on pancreatic beta-cells. Metabolism. 2014, 63: 9-19. 10.1016/j.metabol.2013.09.010.

    CAS  Article  PubMed  Google Scholar 

  4. 4.

    Timmers L, Henriques JP, de Kleijn DP, Devries JH, Kemperman H, Steendijk P, Verlaan CW, Kerver M, Piek JJ, Doevendans PA, Pasterkamp G, Hoefer IE: Exenatide reduces infarct size and improves cardiac function in a porcine model of ischemia and reperfusion injury. J Am Coll Cardiol. 2009, 53: 501-510. 10.1016/j.jacc.2008.10.033.

    CAS  Article  PubMed  Google Scholar 

  5. 5.

    Drucker DJ, Nauck MA: The incretin system: glucagon-like peptide-1 receptor agonists and dipeptidyl peptidase-4 inhibitors in type 2 diabetes. Lancet. 2006, 368: 1696-1705. 10.1016/S0140-6736(06)69705-5.

    CAS  Article  PubMed  Google Scholar 

  6. 6.

    Meloni AR, DeYoung MB, Han J, Best JH, Grimm M: Treatment of patients with type 2 diabetes with exenatide once weekly versus oral glucose-lowering medications or insulin glargine: achievement of glycemic and cardiovascular goals. Cardiovasc Diabetol. 2013, 12: 48-10.1186/1475-2840-12-48.

    PubMed Central  CAS  Article  PubMed  Google Scholar 

  7. 7.

    Paul SK, Maggs D, Klein K, Best JH: Dynamic risk factors associated with non-severe hypoglycaemia in patients treated with insulin glargine or exenatide once weekly.J Diabetes 2014,  : . doi: 10.1111/1753-0407.12208.

  8. 8.

    Mafong DD, Henry RR: Exenatide as a treatment for diabetes and obesity: implications for cardiovascular risk reduction. Curr Atheroscler Rep. 2008, 10: 55-60. 10.1007/s11883-008-0009-z.

    CAS  Article  PubMed  Google Scholar 

  9. 9.

    Brechow A, Slesaczeck T, Münch D, Nanning T, Paetzold H, Schwanebeck U, Bornstein S, Weck M: Improving major amputation rates in the multicomplex diabetic foot patient: focus on the severity of peripheral arterial disease. Ther Adv Endocrinol Metab. 2013, 4: 83-94. 10.1177/2042018813489719.

    PubMed Central  Article  PubMed  Google Scholar 

  10. 10.

    Liu YS, Lu Y, Liu W, Xie H, Luo XH, Wu XP, Yuan LQ: Liao EY:Connective tissue growth factor is a down stream mediator for preptin-induced proliferation and differentiation in human osteoblasts. Amino Acids. 2010, 38: 763-769. 10.1007/s00726-009-0281-4.

    CAS  Article  PubMed  Google Scholar 

  11. 11.

    Yuan LQ, Zhu JH, Wang HW, Liang QH, Xie H, Wu XP, Zhou H, Cui RR, Sheng ZF, Zhou HD, Zhu X, Liu GY, Liu YS, Liao EY: RANKL is a downstream mediator for insulin-induced osteoblastic differentiation of vascular smooth muscle cells. PLoS One. 2011, 6: e29037-10.1371/journal.pone.0029037.

    PubMed Central  CAS  Article  PubMed  Google Scholar 

  12. 12.

    Massy ZA, Drüeke TB: Vascular calcification. Curr Opin Nephrol Hypertens. 2013, 22: 405-412. 10.1097/MNH.0b013e328362155b.

    CAS  Article  PubMed  Google Scholar 

  13. 13.

    Ciceri P, Volpi E, Brenna I, Arnaboldi L, Neri L, Brancaccio D, Cozzolino M: Combined effects of ascorbic acid and phosphate on rat VSMC osteoblastic differentiation. Nephrol Dial Transplant. 2012, 27: 122-127. 10.1093/ndt/gfr284.

    CAS  Article  PubMed  Google Scholar 

  14. 14.

    Zhan JK, Wang YJ, Wang Y, Wang S, Tan P, Huang W, Liu YS: The mammalian target of rapamycin signalling pathway is involved in osteoblastic differentiation of vascular smooth muscle cells. Can J Cardiol. 2014, 30: 568-575. 10.1016/j.cjca.2013.11.005.

    Article  PubMed  Google Scholar 

  15. 15.

    Zhan JK, Wang YJ, Wang Y, Tang ZY, Tan P, Huang W, Liu YS: Adiponectin attenuates the osteoblastic differentiation of vascular smooth muscle cells through the AMPK/mTOR pathway. Exp Cell Res. 2014, 323: 352-358. 10.1016/j.yexcr.2014.02.016.

    CAS  Article  PubMed  Google Scholar 

  16. 16.

    Kaden JJ, Bickelhaupt S, Grobholz R, Vahl CF, Hagl S, Brueckmann M, Haase KK, Dempfle CE, Borggrefe M: Expression of bone sialoprotein and bone morphogenetic protein-2 in calcific aortic stenosis. J Heart Valve Dis. 2004, 13: 560-566.

    PubMed  Google Scholar 

  17. 17.

    Steitz SA, Speer MY, Curinga G, Yang HY, Haynes P, Aebersold R, Schinke T, Karsenty G, Giachelli CM: Smooth muscle cell phenotypic transition associated with calcification upregulation of cbf 1 and downregulation of smooth muscle lineage markers. Circ Res. 2001, 89: 1147-1154. 10.1161/hh2401.101070.

    CAS  Article  PubMed  Google Scholar 

  18. 18.

    Liu H, Lu Q, Huang K: Selenium suppressed hydrogen peroxide-induced vascular smooth muscle cells calcification through inhibiting oxidative stress and ERK activation. J Cell Biochem. 2010, 111: 1556-1564. 10.1002/jcb.22887.

    CAS  Article  PubMed  Google Scholar 

  19. 19.

    Sattler AM, Schoppet M, Schaefer JR, Hofbauer LC: Novel aspects on RANK ligand and osteoprotegerin in osteoporosis and vascular disease. Calcif Tissue Int. 2004, 74: 103-106. 10.1007/s00223-003-0011-y.

    CAS  Article  PubMed  Google Scholar 

  20. 20.

    Bucay N, Sarosi I, Dunstan CR, Morony S, Tarpley J, Capparelli C, Scully S, Tan HL, Xu W, Lacey DL, Boyle WJ, Simonet WS: Osteoprotegerin-deficient mice develop early onset osteoporosis and arterial calcification. Genes Dev. 1998, 12: 1260-1268. 10.1101/gad.12.9.1260.

    PubMed Central  CAS  Article  PubMed  Google Scholar 

  21. 21.

    Min H, Morony S, Sarosi I, Dunstan CR, Capparelli C, Scully S, Van G, Kaufman S, Kostenuik PJ, Lacey DL, Boyle WJ, Simonet WS: Osteoprotegerin reverses osteoporosis by inhibiting endosteal osteoclasts and prevents vascular calcification by blocking a process resembling osteoclastogenesis. J Exp Med. 2000, 192: 463-474. 10.1084/jem.192.4.463.

    PubMed Central  CAS  Article  PubMed  Google Scholar 

  22. 22.

    Panizo S, Cardus A, Encinas M, Parisi E, Valcheva P, López-Ongil S, Coll B, Fernandez E, Valdivielso JM: RANKL increases vascular smooth muscle cell calcification through a RANK-BMP4-dependent pathway. Circ Res. 2009, 104: 1041-1048. 10.1161/CIRCRESAHA.108.189001.

    CAS  Article  PubMed  Google Scholar 

  23. 23.

    Aoki A, Murata M, Asano T, Ikoma A, Sasaki M, Saito T, Otani T, Jinbo S, Ikeda N, Kawakami M, Ishikawa SE: Association of serum osteoprotegerin with vascular calcification in patients with type 2 diabetes. Cardiovasc Diabetol. 2013, 12: 11-10.1186/1475-2840-12-11.

    PubMed Central  CAS  Article  PubMed  Google Scholar 

  24. 24.

    Volpato S, Ferrucci L, Secchiero P, Corallini F, Zuliani G, Fellin R, Guralnik JM, Bandinelli S, Zauli G: Association of tumor necrosis factor-related apoptosis-inducing ligand with total and cardiovascular mortality in older adults. Atherosclerosis. 2011, 215: 452-458. 10.1016/j.atherosclerosis.2010.11.004.

    PubMed Central  CAS  Article  PubMed  Google Scholar 

  25. 25.

    Shan PF, Lu Y, Cui RR, Jiang Y, Yuan LQ, Liao EY: Apelin attenuates the osteoblastic differentiation of vascular smooth muscle cells. PLoS One. 2011, 6: e17938-10.1371/journal.pone.0017938.

    PubMed Central  CAS  Article  PubMed  Google Scholar 

  26. 26.

    Liao XB, Zhou XM, Li JM, Yang JF, Tan ZP, Hu ZW, Liu W, Lu Y, Yuan LQ: Taurine inhibits osteoblastic differentiation of vascular smooth muscle cells via the ERK pathway. Amino Acids. 2008, 34: 525-530. 10.1007/s00726-007-0003-8.

    CAS  Article  PubMed  Google Scholar 

  27. 27.

    Shi Y, Tohyama Y, Kadono T, He J, Miah SM, Hazama R, Tanaka C, Tohyama K, Yamamura H: Protein-tyrosine kinase Sykis required for pathogen engulfment in complement-mediated phagocytosis. Blood. 2006, 107: 4554-4562. 10.1182/blood-2005-09-3616.

    CAS  Article  PubMed  Google Scholar 

  28. 28.

    Callegari A, Coons ML, Ricks JL, Rosenfeld ME, Scatena M: Increased Calcification in Osteoprotegerin-Deficient Smooth Muscle Cells: Dependence on Receptor Activator of NF-κB Ligand and Interleukin 6. J Vasc Res. 2014, 51: 118-131. 10.1159/000358920.

    PubMed Central  CAS  Article  PubMed  Google Scholar 

  29. 29.

    Mai QG, Zhang ZM, Xu S, Lu M, Zhou RP, Zhao L, Jia CH, Wen ZH, Jin DD, Bai XC: Metformin stimulates osteoprotegerin and reduces RANKL expression in osteoblasts and ovariectomized rats. J Cell Biochem. 2011, 112: 2902-2909. 10.1002/jcb.23206.

    CAS  Article  PubMed  Google Scholar 

  30. 30.

    Lee SK, Kalinowski J, Jastrzebski S, Lorenzo JA: 1,25(OH)2 vitamin D3-stimulated osteoclast formation in spleen-osteoblast cocultures is mediated in part by enhanced IL-1 alpha and receptor activator of NF-kappa B ligand production in osteoblasts. J Immunol. 2002, 169: 2374-2380. 10.4049/jimmunol.169.5.2374.

    CAS  Article  PubMed  Google Scholar 

  31. 31.

    Kahn SE: The relative contributions of insulin resistance and beta-cell dysfunction to the pathophysiology of Type 2 diabetes. Diabetologia. 2003, 46: 3-19. 10.1007/s00125-003-1190-9.

    CAS  Article  PubMed  Google Scholar 

  32. 32.

    Xu W, Bi Y, Sun Z, Li J, Guo L, Yang T, Wu G, Shi L, Feng Z, Qiu L, Li Q, Guo X, Luo Z, Lu J, Shan Z, Yang W, Ji Q, Yan L, Li H, Yu X, Li S, Zhou Z, Lv X, Liang Z, Lin S, Zeng L, Yan J, Ji L, Weng J: Comparison of the effects on glycaemic control and β-cell function in newly diagnosed type 2 diabetes patients of treatment with exenatide, insulin or pioglitazone: a multicentre randomized parallel-group trial (the CONFIDENCE study).J Intern Med 2014,  : . doi: 10.1111/joim.12293.

  33. 33.

    Bounthavong M, Tran JN, Golshan S, Piland NF, Morello CM, Blickensderfer A, Best JH: Retrospective cohort study evaluating exenatide twice daily and long-acting insulin analogs in a Veterans Health Administration population with type 2 diabetes. Diabetes Metab. 2014, 40: 284-291. 10.1016/j.diabet.2014.06.002.

    CAS  Article  PubMed  Google Scholar 

  34. 34.

    Kelly AS, Bergenstal RM, Gonzalez-Campoy JM, Katz H, Bank AJ: Effects of exenatide vs metformin on endothelial function in obese patients with pre-diabetes: a randomized trial. Cardiovasc Diabetol. 2012, 11: 64-10.1186/1475-2840-11-64.

    PubMed Central  CAS  Article  PubMed  Google Scholar 

  35. 35.

    Vergès B, Bonnard C, Renard E: Beyond glucose lowering: glucagon-like peptide-1 receptor agonists, body weight and the cardiovascular system. Diabetes Metab. 2011, 37: 477-488. 10.1016/j.diabet.2011.07.001.

    Article  PubMed  Google Scholar 

  36. 36.

    Bose AK, Mocanu MM, Carr RD, Brand CL, Yellon DM: Glucagon-like peptide 1 can directly protect the heart against ischemia/reperfusion injury. Diabetes. 2005, 54: 146-151. 10.2337/diabetes.54.1.146.

    CAS  Article  PubMed  Google Scholar 

  37. 37.

    Arakawa M, Mita T, Azuma K, Ebato C, Goto H, Nomiyama T, Fujitani Y, Hirose T, Kawamori R, Watada H: Inhibition of monocyte adhesion to endothelial cells and attenuation of atherosclerotic lesion by a glucagonlike peptide-1 receptor agonist, exendin-4. Diabetes. 2010, 59: 1030-1037. 10.2337/db09-1694.

    PubMed Central  CAS  Article  PubMed  Google Scholar 

  38. 38.

    Bao W, Holt LJ, Prince RD, Jones GX, Aravindhan K, Szapacs M, Barbour AM, Jolivette LJ, Lepore JJ, Willette RN, DeAngelis E, Jucker BM: Novel fusion of GLP-1 with a domain antibody to serum albumin prolongs protection against myocardial ischemia/reperfusion injury in the rat. Cardiovasc Diabetol. 2013, 12: 148-10.1186/1475-2840-12-148.

    PubMed Central  Article  PubMed  Google Scholar 

  39. 39.

    Ndip A, Williams A, Jude EB, Serracino-Inglott F, Richardson S, Smyth JV, Boulton AJ, Alexander MY: The RANKL/RANK/OPG signaling pathway mediates medial arterial calcification in diabetic Charcot neuroarthropathy. Diabetes. 2011, 60: 2187-2196. 10.2337/db10-1220.

    PubMed Central  CAS  Article  PubMed  Google Scholar 

  40. 40.

    Osako MK, Nakagami H, Shimamura M, Koriyama H, Nakagami F, Shimizu H, Miyake T, Yoshizumi M, Rakugi H, Morishita R: Cross-talk of receptor activator of nuclear factor-κB ligand signaling with renin-angiotensin system in vascular calcification. Arterioscler Thromb Vasc Biol. 2013, 33: 1287-1296. 10.1161/ATVBAHA.112.301099.

    CAS  Article  PubMed  Google Scholar 

  41. 41.

    Mazière C, Salle V, Gomila C, Mazière JC: Oxidized low density lipoprotein enhanced RANKL expression in human osteoblast-like cells. Involvement of ERK, NFkappaB and NFAT. Biochim Biophys Acta. 2013, 1832: 1756-1764. 10.1016/j.bbadis.2013.05.033.

    Article  PubMed  Google Scholar 

  42. 42.

    Hochrainer K, Racchumia G, Anrather J: Hypo-phosphorylation leads to nuclear retention of NF-κB p65 due to impaired IκBα gene synthesis. FEBS Lett. 2007, 581: 5493-5499. 10.1016/j.febslet.2007.10.056.

    PubMed Central  CAS  Article  PubMed  Google Scholar 

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Acknowledgments

This study was supported by the National Natural Science Foundation of China (No.81370931), the National Basic Research Program of China (No. 2014CB910500), the Hunan Provincial Natural Science Foundation of China (No. 13JJ3023), and Hunan Provincial Science and Technology Program (No.2014FJ6065).

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  1. Department of Geriatrics, Institute of Aging and Geriatrics, The Second Xiangya Hospital, Central South University, 139 Middle Renmin Road, Changsha, 410011, Hunan, P.R. China

    Jun-Kun Zhan, Pan Tan, Yan-Jiao Wang, Yi Wang, Jie-Yu He, Zhi-Yong Tang, Wu Huang & You-Shuo Liu

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Zhan, JK., Tan, P., Wang, YJ. et al. Exenatide can inhibit calcification of human VSMCs through the NF-kappaB/RANKL signaling pathway. Cardiovasc Diabetol 13, 153 (2014). https://doi.org/10.1186/s12933-014-0153-4

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Keywords

  • Arterial calcification
  • Vascular smooth muscle cells
  • Osteoblastic differentiation
  • Glucagon-like peptide-1
  • Diabetes

Cardiovascular Diabetology

ISSN: 1475-2840