The effect of exenatide in the phosphorylation of NF-κB and the expression of RANKL in human CVSMCs. A) Human CVSMCs were treated with exenatide (2 nM),1,25VitD3(10-7 M) and BAY11-7082(10 μM) for 48 h. RANKL, NF-κB, p-NF-κB (S529, S276, S536) levels were detected by Western blot. GAPDH was used as the loading control. B) These results were reported at 48 h in each group. Bars represent meanmean ± SD. *P < 0.01 and #P < 0.001 between two indicated groups. N = 5.