Skip to main content
Download PDF
Download ePub

Intense exercise training is not effective to restore the endothelial NO-dependent relaxation in STZ-diabetic rat aorta

Cardiovascular Diabetology volume 12, Article number: 32 (2013) Cite this article

Abstract

Background

The aim of this study was to examine the effects of intense physical training on vascular function in streptozotocin-diabetic rats. We focused on the endothelium-dependent relaxation (EDR) induced by acetylcholine (ACh) and stable ADP adenosine-5′- O – (2-thiodiphosphate) (ADPβS).

Methods

Control or diabetic male Wistar rats (n=44) were randomly assigned to sedentary or trained groups. The training program consisted in a regular period of running on a treadmill during 8 weeks (10° incline and up to 25 m/min, 60 min/day). The reactivity of isolated thoracic aorta rings of healthy, diabetic and/or trained has been tested.

Results

ACh and ADPβS-induced EDR were observed in phenylephrine (PE) pre-contracted vessels. As compared to sedentary control group, diabetic rats showed an increase in PE-induced contraction and a decrease in ACh and ADPβS-induced EDR (p<0.05). Moreover, there were no increase in ACh and ADPβS-induced EDR in diabetic rats. N-Nitro-L-Arginine Methyl Ester inhibited the nitric oxide synthase in diabetic and control rats, thereby resulting in a strong inhibition of the EDR induced by ACh and ADPβS (10-6 M).

Conclusion

Diabetes induced an endothelium dysfunction. Nevertheless, our intense physical training was not effective to restore the aorta endothelial function.

Introduction

The endothelium provides a cellular lining to all blood vessels in the circulatory system and forms a structural barrier between the vascular space and the tissues. Endothelial cells (ECs) regulate vascular flow and basal vasomotor tone by the highly controlled release of vasodilators (nitric oxide (NO) and prostacyclin (PGI2)) and vasoconstrictors [1]. NO is generated in ECs by the oxidation of L-arginine to L-citrulline by the NO synthases (NOS) enzymes family. The endothelial NOS (eNOS) isoform is constitutively active but is further induced by receptor dependent agonist such as thrombin, adenosine 5-diphosphate, bradykinin and substance P [2, 3]. Shear stress also stimulates eNOS activity by virtue of a shear response consensus sequence GAGACC in the eNOS gene promoter [4]. NO has pleiotropic effects on the vasculature. It causes vascular smooth muscle relaxation by binding to guanyl cyclase hence maintaining basal vasomotor tone. It also plays a critical role in the inhibition of thrombosis by inhibiting platelet adhesion activation and agonist-induced secretion. Moderate exercise training is well known to induce beneficial effects by improving Endothelial Function (EF)] and EDR in healthy subjects [5–8]. Efficiency of training depends on both duration and frequency of that type of exercise [2, 9–11]. Training, carried out in variable intensities of exercise, allows authors to note that moderate intensities potentiate the endothelium-dependent-relaxation (EDR) [2].

Diabetes is a risk factor in the development of both macro- and microvascular diseases [12]. It has been established that diabetes is associated with vascular dysfunctions caused by an impairment of EDR [13, 14]. Endothelial dysfunction (ED) is commonly considered as a biomarker of cardiovascular risk and the importance of the endothelium in maintaining normal vascular function is also well recognized [15, 16]. In patients with type 1 or type 2 diabetes, the forearm blood–flow (dilator) responses to ACh are reduced, suggesting an ED [17–19]. In type 1 diabetic animal model, EDR induced by ACh is also impaired. The decrease in NO bioavailability is one of the reasons in this altered-relaxation [20, 21]. Moderate physical activity has been reported to be a major component in the clinical management of diabetic patients [22]. However, the effects of intense exercise training on EF in healthy or pathological subjects are scarce and controversial [23]. Thus, the medical community generally advises diabetic patients to undertake physical activity of moderate intensity (40 to 60% V ˙ O2max) for 20 to 30 minutes at least 3 to 5 times per week. As vigorous physical activity can acutely and transiently increase the risk of cardiovascular events, many practitioners encourage diabetic patients to avoid intense and heavy exercise. However, many type 1 diabetic athletes are known to practice at a high level of competition and to win Olympic medals, leads us to believe that intense sports activity is not necessarily detrimental to type 1 diabetic patient. Moreover, recently, our laboratory showed that intense exercise training combined to insulin treatment had beneficial effects on basal cardiac function and adaptation in expression and responsiveness of cardiac receptors in type 1 diabetic rats [24, 25]. Furthermore, high intensity training can be more effective than moderate intensity training to improve cardiovascular health and clinical outcome in patients with metabolic syndrome [26]. Other data showed that a high-intensity interval training model was effective for improving skeletal muscle mitochondrial capacity and functional exercise performance [27]. Finally, mechanism of the ACh-induced EDR is nowadays well known in diabetic or healthy subjects [28–30], on the contrary, stable ADP adenosine-5′- O – (2-thiodiphosphate) ADPβS-induced EDR mechanisms have been described so far only in healthy rats. The ADPβS-induced relaxation is endothelium-dependent, mediated by P2Y purinoreceptors, at least in part linked to NO and prostacyclin release, and eventually Endothelium-Dependent Hyperpolarizing Factor (EDHF) [31]. To our knowledge, the effects of moderate and intensive training on ADPβS-induced EDR on healthy and diabetic subjects or rats are not documented. This study aimed to evaluate the effects of intense exercise training on EDR in diabetic rats as well as the NO-EDR induced by ACh and ADPβS.

Materials and methods

Experimental model

Animal experiments were conducted according to the Guide for the Care and Use of Laboratory Animals. All experimental protocols were approved by the institutional Animal Care and Use Committee of Rennes University (France) and were carried out following the French Agricultural Ministry’s to the care and use of laboratory Animals. This study meets the ethical standards in sports and exercise science research of the international journal of sports medicine [32]. The study was conducted in male Wistar rats, housed in an animal room on an inverse 12:12-h light-dark cycle (temperature 21 ± 1°C) and given access to water and food ad libitum. The rats (n = 44; from Janvier, France), 9 week-old at the beginning of the experiment, were randomly divided into 4 groups: sedentary control (SC, n = 10), trained control (TC, n = 10), sedentary diabetic (SD, n = 14), and trained diabetic (TD, n = 10).

Induction of experimental streptozotocin (STZ)–induced diabetes

This experimental model of rats made diabetic with streptozotocin (STZ) injection has been validated in previous studies [24]. Animals were injected with either an intraperitoneal single dose of STZ in 0.1 M citrate buffer, pH 4.5 (45 mg/kg) (SD, TD) or citrate buffer only (SC, TC). Three days later, blood glucose levels were determined using a glucometer (MediSense Optium). The onset of diabetes was determined by blood glucose concentration > 250 mg/dL. The detection of ketone bodies, using a glucometer or urinary strips (Keto-Diastix®-Bayer Diagnostic) and body weight loss was used to confirm diabetes.

Exercise training program

One week after the STZ or citrate buffer injection, animals were exercised. Physical training consisted in a progressive running up to 25 m/min, 60 min, 5 days/week (for 8 weeks), as previously validated in our laboratory in same models [24, 25] on a rodent treadmill (Exer 3/6 treadmill, Columbus Instruments) set at an incline of 10°. For the first 2 weeks, each exercise bout consisted of 10 min of running at 20 m/min. The following 3 weeks consisted progressively of 40 min at 22 m/min. for the remaining 3 weeks of the protocol; the duration was increased to 60 min at 25 m/min. Only animals which ran steadily on the treadmill were included in the study. All rats were sacrificed 24 h after the last training session.

Anesthesia & sacrifice

24 to 48 h after the last session of exercise to avoid immediate effects of exercise [2], animals were anesthetized with sodium pentobarbital (50 mg/kg) administrated intraperitoneally. The thoracic aorta were gently removed and immediately stored in a Krebs-Henseleit solution oxygenated and maintained at 37°C (pH 7.4) for vascular analyses. The gastrocnemius tissue was removed and immediately frozen in liquid nitrogen for subsequent analyses.

Citrate synthase activity

Frozen gastrocnemius tissue (200 mg) was used to assay citrate synthase (CS) activity. Muscle samples were homogenized (1/10 w/v) in a buffer solution pH 7.5 containing Na2HPO4 (0.1 mol.L-1), NaH2PO4, H2O (0.1 mol.L-1), and EDTA (2 mmol.L-1) for 20s at 30000 rpm with a polytron. The homogenate was then sonicated 6 x 10s and centrifuged at 1500 G for 13 min at 4°C in duplicate. Citrate synthase activity was spectrophotometrically determined in duplicate in protein extracts at 25°C. Results were expressed in μmol.ml-1.min-1.gTissue-1 for each group. [24, 25].

Preparation of aortic rings for vascular analyses

The removed-thoracic aorta were stored in a Krebs-Henseleit solution oxygenated and maintained at 37°C (pH 7.4) (composition in mM : Nacl 95, KCl 5, MgSO4 1.2, KH2PO4 1.2, NaHCO3 24.9, CaCl2 2.6, glucose 10) and bubbled with 95% O2-5% CO2. The surrounding connective tissue was carefully removed and rings (5 mm width) were prepared. Extreme care was taken not to stretch or damage the luminal surface of the aorta to ensure the integrity of the endothelium. The rings of the proximal portion of the aortic arch were mounted horizontally under isometric conditions in a 15 ml organ bath by inserting two stainless-steel wires into the lumen, according to the Bevan & Osher method (1972) [33]. The tissue isometric tension (g) was recorded by a force-displacement transducer (EMKA Technologies, Paris, France) and an acquisition system (IOx, EMKA Technologies, Paris, France). Preparations were allowed to reach equilibrium for at least 60 minutes under a resting tension of 600-900 mg. Before the beginning of each study, the contractile function was verified by obtaining a KCl-induced contraction and the functional state of the endothelium was evaluated by observation of relaxation to ACh on a ring precontracted with phenylephrine (10-7 M). Only the endothelial intact rings (more than 50% relaxation to Ach) were used.

After the washout (3 times for 20 min), the concentration-response relationship of PE was determined. The percentage of relaxation was evaluated in function of the contraction obtained with a 75% maximal effect of PE concentration. The relaxation study used ACh or ADPβS added in a cumulative manner with an increase of the concentrations (from 10-6 M to 10-5 M for ACh or from 10-8 M to 10-7 M for ADPβS). Finally, the preparations were washed 3 times before the addition of L NAME (10-5 M) until the reach of the basic tension observed (30 min). Then, the same protocol was applied with ACh and ADPβS under N-Nitro-L-Arginine Methyl Ester (L-NAME) conditions. In the L-NAME result section, we will show relaxation data in response to a single dose of ADPβS (10-6 M).

Drugs used

The Acetylcholine (ACh), Phenylephrine (PE), N-Nitro-L-Arginine Methyl Ester (L-NAME) and streptozotocin (STZ) were obtained from Sigma (U.S.A). The Adenosine-5′- O – (2-thiodiphosphate) lithium salt (ADPβS) was from Boehringer Mannheim (Germany). All concentrations are expressed as final molar concentrations in the organ bath.

Statistical analysis

Data are expressed as mean ± SEM; Animal characteristics and CS activity were compared using a 1 way ANOVA and the dose-response curves were compared performed by a 2-way ANOVA for repeated measures followed by Student-Newman-Keuls post-ANOVA tests (Statistica 7.1 software, StatSoft France, 2005). Values of p<0.05 were considered significant.

Results

Animal characteristics

Higher glycemia in SD rats than in SC rats (p<0.001) confirmed the diabetic state. Training reduced but not restored the levels of blood glucose, which were lower in TD rats compared to the SD (p<0.001) (Table 1). Body weights, at the end of the study, were significantly depressed in both SD and TD rats as compared to the SC rats (p<0.001). SC rats had significantly higher body weights than TC rats (p<0.05), whereas SD rats had significantly lower body weights than TD rats (p<0.05) (Table 1). All together, blood glucose values and body weight in diabetic group confirm their pathological status. The levels of gastrocnemius CS activity (mol.ml-1.min-1.gTissue-1) increased significantly (p<0.05) in trained rats compared to their respective sedentary counterparts (Table 1).

Table 1 General characteristics of animals
Full size table

Vasomotricity study

Phenylephrine (PE) Concentrations responses curves

No significant differences were observed between groups TC and SC. TD and SD were significantly different for concentrations from 10-7 M to 6.10-7 M (Figure 1).

Figure 1
figure 1

Concentration dependent constriction of phenylephrine (%). Concentration dependent contraction of phenylephrine (PE) on thoracic aorta rings obtained from sedentary control (SC), trained control (TC), sedentary diabetic (SD) and trained diabetic (TD) groups. Tension is expressed as % relaxation on initial contraction with PE. Values are expressed as mean ±SEM. ‡ Significantly different from sedentary diabetic rats (p<0.05). Significantly different from trained diabetic rats (p<0.05).

Full size image

Effect of diabetes on ACh and ADPβS-induced EDR

We observe that the ACh as ADPβS induced relaxations on aortic rings in healthy and diabetic rats. Relaxations obtained from diabetic rats are significantly reduced compared to control rats. 35.6 ± 0.6% and 26.4 ± 1.5% decreased-relaxation was reported for 10-5 M ACh-induced EDR (Figure 2) for 10-6 M ADPβS-induced EDR (Figure 2), respectively.

Figure 2
figure 2

Concentration dependent relaxation of acetylcholine (%). Concentration dependent relaxation of acetylcholine (ACh) on thoracic aorta rings obtained from sedentary control (SC), trained control (TC), sedentary diabetic (SD) and trained diabetic (TD) groups precontracted with PE. Tension is expressed as % relaxation on initial contraction with PE. Values are expressed as mean ±SEM. * Significantly different from sedentary control rats (p<0.05), † significantly different from trained control rats.

Full size image

Effect of Intense exercise training on ACh and ADPβS- induced EDR in control and STZ-diabetic rats

The concentration-response curves to ACh and ADPβS were not different between TC vs SC and TD vs SD rats (Figures 2 & 3).

Figure 3
figure 3

Concentration dependent relaxation ADPβS (%). Concentration dependent relaxation of ADPβS on thoracic aorta rings obtained from sedentary control (SC), trained control (TC), sedentary diabetic (SD) and trained diabetic (TD) groups precontracted with PE. Tension is expressed as % relaxation on initial contraction with PE. Values are expressed as mean ±SEM. * significantly different from sedentary control rats (p<0.05), † significantly different from trained control rats.

Full size image

Effect of L-NAME on ACh and ADPβS-induced EDR in control and STZ sedentary diabetic rats

The L-NAME (10-6 to 10-5 M) inhibits the relaxations induced by ACh (Figure 4a, b) in both groups (healthy and diabetic). Less than 20% relaxation induced by Ach was still preserved. In the same way, L-NAME combined with ADPβS (10-6 M) limited the EDR to about 20% in both groups (Figure 5).

Figure 4
figure 4

(a) ACh-induced relaxation with and without LNAME in sedentary and trained control. Concentration dependent relaxation of acetylcholine (ACh) with and without LNAME on thoracic aorta rings obtained from sedentary control (SC), trained control (TC) group precontracted with PE. Tension is expressed as % relaxation on initial contraction with PE. Values are expressed as mean ±SEM. * significantly different from sedentary control rats (p<0.05), † significantly different from trained control rats. (b) ACh-induced relaxation with and without LNAME in sedentary and trained STZ-diabetic rats. Concentration dependent relaxation of acetylcholine (ACh) with and without LNAME on thoracic aorta rings obtained from sedentary diabetic (SD) and trained diabetic (TD) groups precontracted with PE. Tension is expressed as % relaxation on initial contraction with PE. Values are expressed as mean ±SEM. ‡ Significantly different from sedentary diabetic rats (p<0.05). Significantly different from trained diabetic rats (p<0.05).

Full size image
Figure 5
figure 5

ADPβS-induced relaxation with and without LNAME. Concentration dependent relaxation of ADPβS with and without LNAME on thoracic aorta rings obtained from sedentary control (SC) and sedentary diabetic (SD) groups precontracted with PE. Tension is expressed as % relaxation on initial contraction with PE. Values are expressed as mean ±SEM. *** = p<0.001 significantly different from (SC). ‡ ‡ ‡ = p<0.001 significantly different from (SD), groups.

Full size image

Discussion

Our study showed that diabetes decrease ACh and ADPβS-induced EDR about 35% and 26% , respectively. In this population, the ADPβS-induced EDR constituted original data. Intensive endurance training did not induce beneficial effects on EDR aorta in control group and did not reverse the vascular dysfunction induced by the diabetic condition. In all groups, under L-NAME, the decrease in ACh or ADPβS-induced EDR confirmed that endothelial relaxations are mediated via NO pathway.

In our diabetic rat model, the higher blood glucose concentration compared to healthy animal one confirmed the absence of insulin secretion subsequent to the STZ-treatment. Our training program lead to the level decrease the level of blood glucose, which was lower in TD compared to the values of their counterparts. Moreover, the drastic decrease in body weight reinforced the diabetic status of animals. Training of diabetic rats led to weight gain due to the increased-muscle mass, reduced weight loss but did not restore a normal body weight such as in healthy animals. Therefore, these observations are in agreement with literature data reporting changes in diabetic (type 1) animals body weight [8, 24]. The CS increase activity demonstrated the efficiency of our training program. The same training program induced a higher increase in CS activity in STZ-diabetic rats that it did in control rats (TD, +56% vs TC, +24% ) (Table 1) because it was probably more intense for diabetic rats [22, 34, 35].

The vasoconstriction increase is induced by PE in STZ-diabetic rats

We observed a significantly increase in contractility induced by PE in diabetic groups as compared to the control ones for concentrations from 10-7 M to 6. 107 M. Therefore, the different concentration-responses curves observed were in agreement with Martinez-Nieves and Dunbar study (1999) [36]. These authors postulated that changes in vascular smooth muscle could be involved in the vascular tone increase observed in diabetic subjects. The mechanism of this potentiation of the PE-induced contraction is due to increasing sensitivity of alpha adrenergic-receptors [36].

The decrease in ACh and ADPβS-induced EDR supports an endothelial dysfunction in STZ-diabetic rats

Diabetes is known for reducing the ACh-induced relaxation [21, 37, 38]. The nature of the studied receptors mediating respectively ACh (Muscarinic (M1/3) receptors) and ADPβS (Purinergic (P2Y) receptors) induced EDR could have an impact on the importance of reducing the EDR in diabetic rats. ADPßS has been shown as an inducer (via P2Y purinergic receptors) of insulin secretion by the beta cells and vasodilatation of pancreatic vascular bed [39]. It is important that along with the secretion of insulin, a vasodilatation must occur. This vasodilatation fosters the effect of insulin and therefore responds to the metabolic and energetic needs. Moreover, if we take into account the physiopathology, we may notice that a dysfunction in the endothelium of pancreatic vascular bed can only perturbate the glycemia regulation. It appeared that the reduction of the EDR was higher in diabetic rats when induced by ACh than by ADPβS, respectively. ACh as ADPβS induced EDR are mediated by NO released from endothelium and consecutively the stimulation of soluble Guanylate cyclase (GMPc). The decrease in NO bioavailability was probably one of the reasons in this relaxation alteration. Therefore, the histamine-induced EDR was potentiated on the diabetic rat [40]. In diabetic rats, the decrease in ADPβS-induced EDR was supported by previous reports [41].

It is obviously noticed that the nucleotides (such as the ADP) -present in the extracellular spaces- contribute to the local regulation of the vascular diameter as well as they play a key role in pathophysiologiacal states. Concerning the ADP and its interaction with the endothelial P2Y1 receptors in a number of diabetic rats type 1 (obtained as a result of STZ treatment), it has been recently proposed that the signalization via NO will be reduced subsequently after an interaction with P2Y1 receptors [42].

In fact, any disturbance of the cellular signalization via the P2 receptors stimulated by ADPßS at the level of the endothelium of pancreatic vascular bed as well as at the level of the cells β, can only lead to a dysfunction regarding the glycemia regulation. A new trend of research appears to remove and rectify this dysfunction targetting the system of signalization mediated by purinergic receptors P2Y.

Indeed, the ADP (as well as ATP, AMP, and adenosine) can hyperpolarize the membrane of the endothelial cell. However, ATP and ADP depolarize the membrane of the smooth muscular cell [43]. As noticed before, associated with the P2Y1 (endothelial receptors) ADP or ADPßS would stimulate the production of EDHF by endothelial cells. In fact, EDHF can induce hyperpolarisation of the vascular smooth muscle membrane thus a vasodilatation would occur. ADPßS is very important for glucose regulation because Hillaire-Buys et al. (2003) [44] reported that ADPßS linked to P2Y was able to alter endothelium-dependent vasorelaxation in vascular pancreatic bed. Specifical agonists as well as ADPßS to P2Y- receptors induced NO/EDRF and EDHF secretion by endothelial cells. EDHF act on vascular smooth muscle cell, openning K+ channel, induce hyperpolarisation and relaxation [40].

Intense exercise training did not potentiate and/or reverse the ACh and ADPβS-induced EDR in control and in STZ-diabetic rats

Our intensive training program did not potentiate and/or reverse the ACh and ADPβS-induced EDR in both groups. This phenomenon could be explained by an excessive ROS production as compared to the positive shear stress effect induced by this exercise. In fact, physical exercise inducing shear stress is the main stimulus of the NO pathway [45]. Shear stress stimulates the membrane receptors sensitized to the stretching of the endothelial cells, and as a result exercise induces vasodilatation. Consequently, EDR should have been greater with training. On the other hand, intense and exhaustive exercises are well known to induce oxidative stress and an excess of ROS production [46]. Previous studies realized on isolated thoracic rat aorta in hypoxic conditions (using drugs) showed that ROS (02°-, 0H-…) alters vasomotricity [47]. NO capturing through the superoxide anion associated with a production of pro-oxidant reactive oxygen species (ROS) can support this hypothesis. Moreover, a correct EDR via the NO°/EDRF pathway requires a coupling between receptors, NOS and cofactor BH4. The inefficiency of our exercise training program to reduce the ED (and enhance EDR) in diabetic rats can be explained by the fact that intense training promotes oxidant stress and leads to decoupling eNOS-cofactor BH4 [17, 35, 48]. Internal data of our lab supported this hypothesis. Indeed, diabetic rats or trained diabetic rats exhibited an hyperglycemic-induced O2°- overproduction, resulting from activation of NOX, eNOS, and Xanthine Oxidase.

In addition, the production of superoxide anion, lactic acid and other molecules may be involved when the shear stress is too high. Consequently, we hypothesized that our endurance training was performed at too great intensity for diabetic population, while for more moderate intensities beneficial effects are typically observed on EDR [15]. Consequently, we can conclude that intense training program was not adapted to reduce an ED in type 1 diabetic rats.

ACh and ADPβS-induced EDRs are mediated via NO pathway in control and STZ-diabetic rats

Our results demonstrated that the NO°/EDRF pathway is involved in the molecular mechanism of these EDR as described in other studies [29, 31]. In particular, we observed that the NO°/EDRF pathway was implicated in the ACh and ADPβS-induced EDR at the same ratio as in control and STZ-diabetic rats. The fraction of ACh and ADPβS-induced EDR not inhibited by L-NAME could be eventually mediated by PGI2 or EDHF [31]. Consequently, it would be interesting to explore the consequences of training in normal or diabetic animals, towards prostacyclin and that of EDHF. It will be intriguing to pursue these investigations particularly with regard to molecular interactions and the metabolism of NO with molecules involved in oxidative stress (superoxide anion, hydroxyl anion, superoxide dismutase, catalase…), as demonstrated in isolated rat thoracic aorta in hypoxic conditions [38, 49]. Classically, it was described that oxidative stress is increased by exercise training [50–52]. When normally at rest, however, a low oxidative stress level is maintained. Obtaining a correct EDR via the NO°/EDRF pathway requires a coupling between receptors, NOS and cofactor BH4. It would be interesting in the future to determine whether the reduction of EDR in diabetic rats is due to BH4-eNOS uncoupling or another molecule.

Conclusion

This study showed that intense exercise training cannot correct endothelial dysfunction, as measured by ACh and ADPβS-induced EDR. EDR did not change in diabetic rats after 8 weeks of intense exercise training. Such findings will most likely recommend the practice of moderate intensities for exercise training to induce benefits on vascular function. It is necessary to pursue a better understanding of the mechanisms of ED in the future.

References

  1. Harrington LS, Lundberg MH, Rozario A, Mitchell JA: Reduced endothelial dependent vasodilation in vessels from TL4−/−mice is associated with increased superoxide generation. Cardiothoracic Pharmacology. 2011, 5: 408-511.

    Google Scholar 

  2. Heylen E, Guerrero F, Mansourati J, Theron M, Thibou S, Saïag B: Effect of training frequency on endothelium-dependent vasorelaxation in rat. Eur J Cardiovasc Prev Rehabil. 2008, 15: 52-8. 10.1097/HJR.0b013e3282f17baa.

    Article  PubMed  Google Scholar 

  3. Pellegrin M, Bertholot A, Houdayer C, Gaume V, Deckert V, Laurent P: New insights into the vascular mechanisms underlying the beneficial effect of swimming training on the endothelial vasodilator function in apolipo-protein E-deficient mice. Atherosclerosis. 2007, 190: 35-42. 10.1016/j.atherosclerosis.2006.02.001.

    CAS  Article  PubMed  Google Scholar 

  4. Venema RC, Nishida K, Alexander RW, Harrison DG, Murphy TJ: Organization of the bovine gene encoding the endothelial nitric oxide synthase. Biochim Biophys Acta. 1994, 1218 (3): 413-20. 10.1016/0167-4781(94)90195-3.

    CAS  Article  PubMed  Google Scholar 

  5. De Moraes C, Davel AP, Rossoni LV, Antunes E, Zanesco A: Exercise training improves relaxation response and SOD-1 expression in aortic and mesenteric rings from high caloric diet-fed rats. BMC Physiol. 2008, 8: 12-10.1186/1472-6793-8-12.

    PubMed Central  Article  PubMed  Google Scholar 

  6. Boraita Pérez A: Exercise as the cornerstone of cardiovascular prevention. Rev Esp Cardiol. 2008, 61: 514-528. 10.1157/13119996.

    Article  PubMed  Google Scholar 

  7. Delaney LE, Arce-Esquivel AA, Kuroki K, Laughlin MH: Exercise training improves vasoreactivity in the knee artery. Int J Sports Med. 2012, 33: 114-22.

    PubMed Central  CAS  Article  PubMed  Google Scholar 

  8. DeSouza CA, Shapiro LF, Clevenger CM, Dinenno FA, Monahan KD, Tanaka H, Seals DR: Regular aerobic exercise prevents and restores age-related declines in endothelium-dependent vasodilation in healthy men. Circulation. 2000, 102: 1351-7. 10.1161/01.CIR.102.12.1351.

    CAS  Article  PubMed  Google Scholar 

  9. Haram PM, Adams V, Kemi OJ, Brubbakk AO, Hambrecht R, Ellingsen O: Time-course of endothelial adaptation following acute and regular exercise. Eur J Cardiovasc Prev Rehabil. 2006, 13: 585-91. 10.1097/01.hjr.0000198920.57685.76.

    Article  PubMed  Google Scholar 

  10. Heylen E, Simon B, Guerrero F, Elkaïm JP, Saïag B, Mansourati J: Reactive hyperaemia in the forearm skin of highly trained windsurfers. Int J Sports Med. 2005, 26: 822-6. 10.1055/s-2005-837452.

    CAS  Article  PubMed  Google Scholar 

  11. Spier SA, Delp MD, Meininger CJ, Donato AJ, Ramsey MW, Muller-Delp JM: Effects of ageing and exercise training on endothelium-dependent vasodilatation and structure of rat skeletal muscle arterioles. J Physiol. 2004, 556: 947-58. 10.1113/jphysiol.2003.060301.

    PubMed Central  CAS  Article  PubMed  Google Scholar 

  12. Xue G, Luis A, Martinez L, Zhang C: Endothelium-derived hyperpolarizing factor and diabetes. World J Cardiol. 2011, 3: 25-31. 10.4330/wjc.v3.i1.25.

    Article  Google Scholar 

  13. Bakker W, Eringo EC, Sipkema P, Van Hinsbergh VW: Endothelial dysfunction and diabetes: roles of hyperglycemia, impaired insulin signaling and obesity. Cell Tissue Res. 2009, 335: 165-189. 10.1007/s00441-008-0685-6.

    CAS  Article  PubMed  Google Scholar 

  14. Teixeira-Lemos E, Nunes S, Teixeira F, Reis F: Regular physical exercise training assists in preventing type 2 diabetes development: focus on its antioxidant and anti-inflammatory properties. Cardiovasc Diabetol. 28, 10: 12.

    Article  Google Scholar 

  15. Deanfield JE, Halcox JP, Rabelink TJ: Endothelial function and dysfunction: testing and clinical relevance. Circulation. 2007, 115: 128-95.

    Google Scholar 

  16. Maiorana A, O’Driscoll G, Dembo L, Goodman C, Taylor R, Green D: Exercise training, vascular function, and functional capacity in middle-aged subjects. Med Sci Sports Exerc. 2001, 33: 2022-8. 10.1097/00005768-200112000-00008.

    CAS  Article  PubMed  Google Scholar 

  17. Delp MD, Behnke BJ, Spier SA, Wu G, Muller-Delp JM: Ageing diminishes endothelium-dependent vasodilatation and tetrahydrobiopterin content in rat skeletal muscle arterioles. J Physiol. 2008, 586: 1161-8.

    PubMed Central  CAS  Article  PubMed  Google Scholar 

  18. Endemann DH, Schiffrin EL: Endothelial dysfunction. J Am Soc Nephrol. 2004, 15: 1983-92. 10.1097/01.ASN.0000132474.50966.DA.

    CAS  Article  PubMed  Google Scholar 

  19. Gewaltig MT, Kojda G: Vasoprotection by nitric oxide: mechanisms and therapeutic potential. Cardiovasc Res. 2002, 55: 250-60. 10.1016/S0008-6363(02)00327-9.

    CAS  Article  PubMed  Google Scholar 

  20. Mustafa S, Sharma V, McNeill JH: Insulin resistance and endothelial dysfunction: Are epoxyeicosatrienoic. Exp Clin Cardiol. 2009, 14: 41-50.

    Google Scholar 

  21. Olukman E, Orhan CE, Gul Celenk F, Ulke S: Apocynin restores endothelial dysfunction in streptozotocin diabetic rats through regulation of nitric oxide synthase and NADPH oxidase expressions. J Diabetes Complications. 2010, 4: 415-423.

    Article  Google Scholar 

  22. Heyman E, Toutain C, Delamarche P, Berthon P, Briard D, Youssef H: Exercise training and cardiovascular risk factors in type 1 diabetic adolescent girls. Pediatr Exerc Sci. 2007, 19: 408-419.

    PubMed  Google Scholar 

  23. Walsh JH, Bilsborough W, Maiorana A, Best M, O’Driscoll GJ, Taylor RR, Green DJ: Exercise training improves conduit vessel function in patients with coronary artery disease. J Appl Physiol. 2003, 95: 20-5.

    Article  PubMed  Google Scholar 

  24. Le Douairon LS, Gratas-Delamarche A, Malardé L, Vincent S, Zguira MS, Vincent S, Lemoine Morel S, Drlamarche P, Zouhal H, Carré F, Bekono FR: Intense exercise training induces adaptation in expression and responsiveness of cardiac β-adrenoceptors in diabetic rats. Cardiovasc Diabetol. 2010, 9: 72-10.1186/1475-2840-9-72.

    Article  Google Scholar 

  25. Le Douairon LS, Rebillard A, Zguira MS, Malardé L, Saïag B, Gratas-Delamarche A, Carré F, Bekono FR: Effects of exercise training combined with insulin treatment on cardiac NOS1 signaling pathways in type 1 diabetic rats. Mol Cell Biochem. 2011, 347: 53-62. 10.1007/s11010-010-0611-6.

    Article  Google Scholar 

  26. Tjønna AE, Lee SJ, Rognmo Ø, Stølen TO, Bye A, Haram PM, Loennechen JP, Al-Share QY, Skogvoll E, Slørdahl SA, Kemi OJ, Najjar SM, Wisløff U: Aerobic interval training versus continuous moderate exercise as a treatment for the metabolic syndrome: a pilot study. Circulation. 2008, 118: 346-354. 10.1161/CIRCULATIONAHA.108.772822.

    PubMed Central  Article  PubMed  Google Scholar 

  27. Little JP, Safdar A, Wilkin GP, Tarnopolsky MA, Gibala MJ: A practical model of low-volume high-intensity interval training induces mitochondrial biogenesis in human skeletal muscle: potential mechanisms. J Physiol. 2010, 588: 1011-1022. 10.1113/jphysiol.2009.181743.

    PubMed Central  CAS  Article  PubMed  Google Scholar 

  28. Heitzer T, Finch B, Albers S, Krohn K, Kohlschütter A, Meinertz T: Beneficial effects of alpha-lipoic acid and ascorbic acid on endothelium-dependent, nitric oxide-mediated vasodilation in diabetic patients: relation to parameters of oxidative stress. Free Radic Biol Med. 2001, 31: 53-61. 10.1016/S0891-5849(01)00551-2.

    CAS  Article  PubMed  Google Scholar 

  29. Furchgott RF, Zawadzki JV: The obligatory role of endothelial cells in the relaxation of arterial smooth muscle by acetylcholine. Nature. 1980, 288: 373-6. 10.1038/288373a0.

    CAS  Article  PubMed  Google Scholar 

  30. Minami A, Ishimura N, Harada N, Sakamoto S, Niwa Y, Nakaya Y: Exercise training improves acetylcholine-induced endothelium-dependent hyperpolarization in type 2 diabetic rats, Otsuka Long-Evans Tokushima fatty rats. Atherosclerosis. 2002, 162: 85-92. 10.1016/S0021-9150(01)00685-2.

    CAS  Article  PubMed  Google Scholar 

  31. Saiag B, Hillaire-Buys D, Chapal J, Petit P, Pape D, Rault B, Allain H, Loubatières-Mariani MM: Study of the mechanisms involved in adenosine-5′-O-2(2-thiodiphosphate induced relaxation of rat thoracic aorta and pancreatic vascular bed. Br J Pharmacol. 1996, 118: 804-10. 10.1111/j.1476-5381.1996.tb15471.x.

    PubMed Central  CAS  Article  PubMed  Google Scholar 

  32. Harriss DJ, Atkinson G: Update - ethical standards in sport and exercise science research. Int J Sports Med. 2011, 32: 819-821.

    CAS  Article  PubMed  Google Scholar 

  33. Bevan , Osher : A direct method for recording tension changes in the wall of small blood. Agents Action. 1972, 2: 257-60. 10.1007/BF02087051.

    CAS  Article  Google Scholar 

  34. Gratas-Delamarche A, Heyman E: Effects of type 1 diabetes and of training on physical fitness. Science Sports. 2006, 21: 57-61. 10.1016/j.scispo.2005.06.010.

    Article  Google Scholar 

  35. Rush JW, Laughlin MH, Woodman CR, Price EM: SOD-1 expression in pig coronary arterioles is increased by exercise training. Am J Physiol Heart Circ Physiol. 2000, 279: 2068-2076.

    Google Scholar 

  36. Martinez-Nieves B, Dunbar J: Cardio-Vascular dilatory responses to sodium Nitroprusside (SNP) and Alpha –Adrenergic antagonism in female and male normal and diabetic rats. Proc Soc Exp Biol Med. 1999, 222: 90-8. 10.1111/j.1525-1373.1999.10000.x.

    CAS  Article  PubMed  Google Scholar 

  37. Junqueiro D, Rival Y: Metabolic syndrome: which definition for what treatment(s)?. Med Sci. 2005, 21: 1045-53.

    Google Scholar 

  38. Katsou K, Noriyuki M, Yutaka K: Impairment of endothelium - dependent relaxation and changes in levels of cyclic GMP in aorta from streptozotocin-induced diabetic rats. Br J Pharmacol. 1989, 97: 614-618. 10.1111/j.1476-5381.1989.tb11993.x.

    Article  Google Scholar 

  39. Chapal J, Hillaire-Buys D, Bertrand G, Pujatte D, Petit P, Loubatières-Mariani MM: Comparative effects of adenosine-5′-triphosphate and related analogues on insulin secretion from the rat pancreas. Fundam Clin Pharmacol. 1997, 11: 537-45. 10.1111/j.1472-8206.1997.tb00858.x.

    CAS  Article  PubMed  Google Scholar 

  40. Matsumoto T, Yoshiyama S, Wakabayashi K, Kobayachi T, Kamata K: Effect of chronic insulin on cromakalim-induced relaxation in established streptozotocin-diabetic rat basilar artery. Eur J Pharmacol. 2004, 504: 119-127. 10.1016/j.ejphar.2004.09.044.

    CAS  Article  PubMed  Google Scholar 

  41. Csanyi G, Lepran I, Flesch T, Telegdy G, Szabo G, Mezei Z: Lack of endothelium-derived hyperpolarizing factor (EDHF) up-regulation in endothelial dysfunction in aorta in diabetic rats. Pharmacol Rep. 2007, 59: 447-455.

    CAS  PubMed  Google Scholar 

  42. Ishida K, Matsumoto T, Taguchi K, Kamata K, Kobayashi T: Mechanisms underlaying reduced P2Y(1)-receptor-mediated relaxation in superior mesenteric arteries from long-term streptozotocin-induced diabetic rats. Acta Physiol (Oxf). 2013, 207: 130-41. 10.1111/j.1748-1716.2012.02469.x.

    CAS  Article  Google Scholar 

  43. Chen G, Suzuki H: Endothelium-dependent hyperpolarization elicited by adenine compounds in rabbit carotid artery. Am J Physiol. 1991, 260: 1037-42.

    Google Scholar 

  44. Hillaire-Buys D, Peyriere H, Linck N, Saïag B: Dysfonctionnement endothélial et diabète: aspect fondamentaux et perspectives thérapeutiques. Angeiologie. 2003, 55: 33-40.

    Google Scholar 

  45. Laughlin MH, Oltman CL, Bowles DK: Exercise training-induced adaptations in the coronary circulation. Med Sci Sports Exerc. 1998, 30: 352-60. 10.1097/00005768-199803000-00004.

    CAS  Article  PubMed  Google Scholar 

  46. Groussard C, Machefer G, Rannou F, Zouhal H, Sergent O, Chevanne M, Cillard J, Gratas-Delamarche A: Physical fitness and plasma non-enzymatic antioxidant status at rest and after a wingate test. Can J Appl Physiol. 2003, 28: 79-92. 10.1139/h03-007.

    CAS  Article  PubMed  Google Scholar 

  47. Saiag B, Shacoori V, Bodin P, Catheline M, Pape D, Alain H, Burnstock G: Pharmacological evidences for free radical implications in endothelium dependent hypoxic responses of the rat thoracic aorta. Eur J Pharmacol. 1999, 372: 57-63. 10.1016/S0014-2999(99)00198-3.

    CAS  Article  PubMed  Google Scholar 

  48. Sindler AL, Delp MD, Reyes R, Wu G, Muller-Delp JM: Effects of ageing and exercise training on eNOS uncoupling in skeletal muscle resistance arterioles. J Physiol. 2009, 587: 3885-3897. 10.1113/jphysiol.2009.172221.

    PubMed Central  CAS  Article  PubMed  Google Scholar 

  49. Saïag B, Milon D, Allain H, Rault B, Van den Driessch J: Constriction of the smooth muscle of rat tail and femoral artriesand dog saphenous vein is induced by uridine triphosphate via pyrimidinoceptors, and by adenosine triphosphate via P2X purinoreceptors. Blood Vessels. 1990, 27: 352-64.

    PubMed  Google Scholar 

  50. Alessio HM: Exercise-induced oxidative stress. Med Sci Sports Exerc. 1993, 25: 218-224.

    CAS  Article  PubMed  Google Scholar 

  51. Alessio HM, Hagerman AE, Fulkerson BK, Ambrose J, Rice RE, Wiley RL: Generation of reactive oxygen species after exhaustive aerobic and isometric exercise. Med Sci Sports Exerc. 2000, 32: 1576-1581.

    CAS  Article  PubMed  Google Scholar 

  52. Daio S, Fraser H, Yuen VG, McNeill JH: Improvement in cardiac function in streptozotocin-diabetic rats by salt loading. Can J Phys Pharmacol. 1994, 11: 1313-1471.

    Google Scholar 

Download references

Acknowledgments

We gratefully thank the regional association of endothelium research (ARRE), the Langlois Foundation (West Foundation), Prof Paul Delamarche, Prof Arlette Gratas-Delamarche, Prof Alain Legrand, for assistance, Prof François Carréand Prof Eric Bellissant for their home, Mrs Catherine Lemeslif, Christine Dutais and Marie–José Ferrand-Sorre from the laboratory of Medical Physiology (Faculty of Medecine, Rennes 1, France) for their technical assistance

Author information

Affiliations

  1. Laboratory “Movement Sport and health Sciences”, UFR APS University of Rennes 2, Avenue Charles Tillon, Rennes cedex, 35044, France

    Mohamed Sami Zguira, Sophie Vincent, Solène Le Douairon Lahaye, Ludivine Malarde & Bernard Saïag

  2. Laboratory “Movement Sport and health Sciences”, ENS Cachan –antenne de Bretagne, Campus Ker Lann, Bruz, France

    Mohamed Sami Zguira, Sophie Vincent, Solène Le Douairon Lahaye, Ludivine Malarde & Bernard Saïag

  3. Clinical Laboratory of Physiology, Medical School of Sousse, Sousse, Tunisia

    Mohamed Sami Zguira & Zouhair Tabka

Authors
  1. Mohamed Sami Zguira
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Sophie Vincent
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Solène Le Douairon Lahaye
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Ludivine Malarde
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Zouhair Tabka
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Bernard Saïag
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Mohamed Sami Zguira.

Additional information

Competing interests

The authors declare that they have no competing interests.

Authors’ contributions

MSZ was responsible for the design conception of the experiments, collection, analysis and interpretation of the data, and drafting of the manuscript. SV was responsible for the conception of the experiment and collection of the data. SLDL and LM were responsible for exercise training protocol. ZT has participated in interpretation of the data. BS supervised the study and was responsible for the design conception of the experiments, interpretation of the data, and drafting of the manuscript. All authors have read and approved the final manuscript.

Authors’ original submitted files for images

Rights and permissions

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Reprints and Permissions

About this article

Cite this article

Zguira, M.S., Vincent, S., Le Douairon Lahaye, S. et al. Intense exercise training is not effective to restore the endothelial NO-dependent relaxation in STZ-diabetic rat aorta. Cardiovasc Diabetol 12, 32 (2013). https://doi.org/10.1186/1475-2840-12-32

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI: https://doi.org/10.1186/1475-2840-12-32

Keywords

  • Endothelial relaxation
  • Training
  • Acetylcholine
  • ADPβS
  • Diabetic rat

Cardiovascular Diabetology

ISSN: 1475-2840