Galectin-3 deficiency exacerbates hyperglycemia and the endothelial response to diabetes
© Darrow and Shohet. 2015
Received: 16 March 2015
Accepted: 23 May 2015
Published: 6 June 2015
Diabetes promotes maladaptive changes in the endothelium that lead to its dysfunction and contribute to the vascular pathology of diabetes. We have previously reported the up-regulation of galectin-3, a β-galactoside-binding lectin, in the endothelium and sera of diabetic mice, implicating this molecule in diabetic vasculopathy and suggesting its potential as a biomarker of the disease. Therefore, we sought to assess the role of galectin-3 in the vascular pathology of diabetes.
Galectin-3 knockout mice (KO) and wild-type mice (WT) were fed either a high-fat diet (HFD) (60 % fat calories) to produce insulin resistant diabetes, or standard chow (12 % fat calories), and their metabolic and endothelial responses were measured. After 8 weeks, the aortic and skeletal muscle endothelia were isolated by fluorescence sorting of CD105+/CD45− cells and comprehensive transcriptional analyses were performed. Transcripts differentially dysregulated by HFD in KO endothelium compared to WT were confirmed by semi-quantitative RT-PCR, and protein expression was determined by immunofluorescence of aortic and muscle tissue. Ingenuity® Pathway Analysis was used to identify pathways up-regulated by HFD in the KO, such as the coagulation cascade, and measurements of blood clotting activity were performed to confirm these results.
KO mice exhibit greater hyperglycemia and impaired glucose tolerance but lower insulin levels on HFD compared to WT. KO mice demonstrate a more robust transcriptional response to HFD in the vascular endothelium compared to WT. Transcripts dysregulated in the KO endothelium after HFD are involved in glucose uptake and insulin signaling, vasoregulation, coagulation, and atherogenesis. One of the most down-regulated transcripts in the endothelium of the KO after HFD was the glucose transporter, Glut4/Slc2a4. GLUT4 immunofluorescence confirmed lower protein abundance in the endothelium and muscle of the HFD-fed KO. Prothrombin time was decreased in the diabetic KO indicating increased coagulation activity.
Galectin-3 deficiency leads to exacerbated metabolic derangement and endothelial dysfunction. The impaired tissue uptake of glucose in KO mice can be attributed to the reduced expression of GLUT4. Enhanced coagulation activity in the diabetic KO suggests a protective role for galectin-3 against thrombosis. These studies demonstrate that galectin-3 deficiency contributes both to the pathogenesis of diabetes and the associated vasculopathy.
Diabetes promotes maladaptive vascular responses in numerous tissues, including changes in the endothelium that contribute to insulin resistance and ultimately, the vascular pathology of diabetes. Endothelial damage or dysfunction is the primary cause of the macrovascular and microvascular complications associated with diabetes. Previously, we have developed methods for the rapid isolation of highly purified endothelial cells from mice exposed to models of diabetes [1, 2]. Transcriptional analysis of these diabetic endothelial cells revealed increased abundance of galectin-3 mRNA and protein in the aortic and muscle endothelium. We also found a correlation between serum galectin-3 levels and insulin resistance, implicating this molecule as a potential biomarker of diabetic vasculopathy . Human studies have confirmed an elevation of systemic galectin-3 in type II diabetic patients compared to healthy individuals . A recent clinical study has shown that galectin-3 levels correlate with plasma glucose, C-reactive protein, and degree of insulin resistance, suggesting its potential as a biomarker for predicting diabetes and prediabetes . Higher galectin-3 levels have also been associated with increased risk of vascular complications . In light of our preliminary findings, we suspected that galectin-3 is involved in the endothelial dysfunction that leads to the vascular complications of type II diabetes. Thus, we sought to determine the role of galectin-3 in the vascular pathology and metabolic derangement observed in type II diabetes.
In recent years, the importance of this molecule in disease pathology and its potential as a therapeutic target has become increasingly evident. Galectin-3 has been studied for putative roles in heart failure, fibrosis, cancer, angiogenesis, and atherosclerosis. Human studies show that subjects with elevated galectin-3 levels have increased risk for developing heart failure, and blood testing for galectin-3 is now being used to monitor heart failure [6–8]. Perhaps similar testing could be used to predict vascular complications in diabetic patients.
Intracellular LGALS3 is involved in pre-mRNA splicing and has been implicated in the regulation of gene transcription by stabilizing transcription factor binding [9, 10]. Extracellular LGALS3 interacts with components of the extracellular matrix as well as cell-surface carbohydrates to influence cell adhesion and migration. LGALS3 is up-regulated on the endothelial cell surface during adherence and migration of polymorphonuclear leukocytes in vitro  and may also be involved in mediating NG2 proteoglycan-induced motility leading to the recruitment of endothelial cells to sites of neovascularization . Galectin-3 is also a component of the advanced glycation endproduct (AGE)–receptor complex expressed on the surface of endothelial cells, which is linked to the binding and removal of AGEs from the circulation . CHO cells overexpressing LGALS3 specifically bind AGE-BSA and modified LDLs, leading to their endocytosis . Galectin-3 deficient mice have been shown to display increased glomerular accumulation of AGEs in a model of type I diabetes and increased ox-LDL and lipoprotein products when fed an atherogenic diet [15, 16].
The galectin-3 knockout has been used to study the role of galectin-3 in murine models of type I diabetes induced by streptozotocin, where both pro-diabetogenic and protective roles have been reported [15, 17]. Galectin-3 protein expression increases in human atherosclerotic lesions as well as in the aortae of experimental animal models of diabetes [18, 19]. Galectin-3 deletion or inhibition on the ApoE (−/−) background has been shown to reduce atherosclerotic lesions and plaques [18, 20], while galectin-3 knockout mice fed an atherogenic diet for 8 months showed increased lesion area and length compared to wild-type mice . These studies suggest an important role for galectin-3 in vascular complications. However, the conflicting reports regarding its role in diabetes and its associated vasculopathy emphasize the need for further evaluation.
The goal of our study was to identify the role of this multifunctional lectin in the development of endothelial dysfunction induced by type II diabetes. To do this, we examine the metabolic changes and the endothelial transcriptional response to a high-fat diet in wild-type and Lgals3-deficient mice. These studies define the role of galectin-3 in endothelial dysfunction and insulin resistance and help to elucidate the pathogenesis of diabetic vasculopathy.
Animals and diet
Mice homozygous for a targeted deletion within the galectin-3 gene (KO) [B6.Cg-Lgals3 tm1Poi /J, stock no. 006338 Jackson Laboratories (Bar Harbor, ME)] and wild-type C57BL/6 J (WT) (stock no. 000664) were bred for these experiments. The galectin-3 knockout was generated by a 3.7 kb deletion of exons 2–4, including the initiating codon in exon 2 as previously described . Genetic deletion of galectin-3 was confirmed at the DNA level by gel electrophoresis and also at the RNA and protein level by real-time PCR and Western blotting (Additional file 1).
Beginning at 8 weeks of age, male KO and WT mice were allowed to feed ad libitum on a high-fat diet (HFD) containing 60 % fat calories (BioServ, Frenchtown, NJ, cat. no. S3282) or a normal chow diet containing 12 % fat calories (LabDiet, St. Louis, MO, cat. no. 5001) for a period of 8 weeks. All procedures were approved by the Institutional Animal Care and Use Committee of the University of Hawaii.
Blood glucose and serum insulin levels were measured before the start of the diet and after 2, 4, 6, and 8 weeks on their respective diets. Animals were fasted overnight, and glucometry of tail blood was performed using an OneTouch Ultra (Lifescan, Milpitas, CA). Two-hundred μL of blood was collected from the tail vein, and insulin levels in the separated serum were determined by ELISA (Mercodia, Sweden). A glucose tolerance test (GTT) was performed after 5 weeks on the diet. Glucose (1 mg/g of body weight) was administered i.p. following an overnight fast, and glucometry of the tail blood was performed prior to glucose injection and every 20 minutes afterwards for 2 hrs. The homeostatic model assessment of insulin resistance (HOMA-IR) was calculated using the following formula: [fasting blood glucose (mg/dL) × fasting insulin (μIU/mL)] / 405 , where 1 mg insulin = 26 IU .
Advanced glycation end products (AGEs) in the serum were measured after 8 weeks of diet. Blood was collected by cardiac puncture and serum was separated and diluted to obtain a protein concentration of 5 mg/mL. AGEs were quantified by ELISA (Wuhan EIAAB Science, China, cat.no.E0263m) and normalized to total protein.
Measurement of AKT phosphorylation
Following 8 weeks on the diet, chow and high-fat fed mice of each strain received an i.p. injection of insulin (0.06 U/g body weight in 300 μL of sterile saline); 1–2 animals of each group received vehicle (normal saline). Fifteen minutes after insulin challenge, animals were sacrificed and thoracic aorta and skeletal muscle were harvested and homogenized as previously described . Abundance of phosphorylated AKT and total AKT were determined by Western Blotting. Briefly, denatured aorta or muscle protein was separated on 12 % Bis-Tris polyacrylamide gels under reducing conditions. Following transfer and blocking, membranes were incubated overnight in rabbit anti-mouse phospho-AKT antibody (serine 473) (Cell Signaling, Beverly, MA, cat.no.9271) at 4 °C and visualized with an Alexa Fluor®568-conjugated secondary antibody (Invitrogen, Carlsbad, CA) on a Typhoon® phosphorimager (Amersham). Blots were then stripped and reprobed for total AKT (Cell Signaling, cat.no.9272). Band intensity was quantified by densitometry using ImageJ , and phosphorylated AKT was normalized to total AKT levels.
Endothelial cell isolation
Following 8 weeks on the diet regimen, animals were sacrificed by CO2 asphyxiation. In each experiment, for each diet, pooled cells from 3 KO and 3 WT mice were collected for each tissue. Aortae from the aortic root to the iliac bifurcation were dissected. Leg muscles consisting of the plantaris, gastrocnemius, and biceps femoris (which are readily dissected as a single group) were excised. Tissues were placed into ice cold PBS and freed of adherent fat. The aortic and skeletal muscle tissues from 3 animals were each pooled, minced into 1 mm fragments, and dispersed as previously described . Suspensions of collagenolytically separated cells were incubated with anti-mouse CD16/32 (1:500) for 5 min and then with phycoerythrin-conjugated anti-mouse CD45 (1:800) and anti-mouse CD105 eFluor®450 (1:20) for 25 minutes on ice (eBiosciences, cat.nos.14-0161, 17–0451, and 48–1051). Immediately before sorting, 5 μl of Dead Cell Discriminator (Life Technologies, Carlsbad, CA) was added per 100 μl of cell suspension. Roughly 10,000 live endothelial cells positive for CD105 staining and negative for CD45 were isolated with a FACSAria (Becton Dickinson, Franklin Lakes, NJ) directly into Trizol (Invitrogen, Carlsbad, CA). RNA was extracted and purified with RNeasy columns (Qiagen, Valencia, CA) and then amplified using an Ambion® Amino Allyl MessageAmp kit (Life Technologies, Carlsbad, CA) according to the manufacturer’s protocol to produce approximately 100 μg of amino-allyl modified cRNA.
Microarray analyses were performed to determine the transcriptional responses in aortic and skeletal muscle endothelium from KO vs. WT mice exposed to either high-fat diet or control diet for 8 weeks. Amino-allyl modified cRNA was labeled with Cy3 CyDye™ Post-Labeling Reactive Dye Pack (GE Healthcare, Waukesha, WI) according to the manufacturer’s instructions. Following purification, 1.6 μg of Cy3 dye-labeled cRNA, as measured by NanoDrop 2000c spectrophotometer (Thermo Scientific, Bellerica, MA), were combined and fragmented. Biological triplicate experiments were performed for both tissues and both diets for each strain. Samples were hybridized overnight to glass slides spotted with the Whole Mouse Genome Array Kit from Agilent (Santa Clara, CA, cat. no. G4122F), which includes 44,000 murine oligonucleotides representing all known genes and transcripts of the mouse genome along with positive controls. The set of 3’ biased, 60-mer oligos are synthesized in situ using Agilent SurePrint technology. Annealed fluorescent labels were quantified with Agilent dual channel scanner, and data analysis subsequently performed with GeneSpring® and Acuity® software. For both the KO and WT strains, transcripts exhibiting up-regulation (a log2 [fold change] >1) or down-regulation (log2 [fold change] < −1) by HFD vs. chow diet that were found consistently dysregulated in all 3 biologically replicate experiments were tabulated for each tissue. This comparison represents the endothelial response to diabetes in either strain.
The gene expression data was further interrogated using QIAGEN’s Ingenuity® Pathway Analysis (IPA®, QIAGEN Redwood City, www.qiagen.com/ingenuity). Datasets consisting of all transcripts differentially expressed >0.75 log2 [fold change] by high-fat feeding in the WT and KO strains were uploaded into IPA®. Over-represented Biological Functions in the aortic endothelium of each strain after HFD were identified based on weighted gene co-expression. IPA was also performed on differentially regulated transcripts between KO HFD and WT HFD animals to identify differential Canonical Pathways due to galectin-3 ablation in addition to the response to HFD.
Microarray results were confirmed by semi-quantitative real-time PCR (qPCR) performed on 3 biological replicate experiments. Synthesis of cDNA was obtained by reverse transcription of 1 μg of amplified RNA from the sorted endothelial cells using qScript (Quanta Biosciences, Gaithersburg, MD). Oligonucleotide primers were designed to generate amplicons 100–200 nucleotides long which spanned at least one intron. Primer sequences are listed in Additional file 2. The cDNA representing 5 ng of total RNA was amplified by PCR performed using SYBR® green fluorophore (Roche, Indianapolis, IN) in an Applied Biosystems® 7900HT fast real-time PCR system. A standard two-phase reaction (95 °C 15 sec, 60 °C 1 min) worked for all amplifications. Dissociation curves run for each reaction verified the presence of a single amplicon peak. Amplicons were also sequenced by 3730XL DNA Analyzer (Applied Biosystems), and BLAST was used to verify the alignment of the amplicon sequence with that of the target transcript (Additional file 2).
Fold changes represent the ratio of HFD to chow diet expression values determined from the cycle times where CT, the threshold intensity, was exceeded. The abundance of Cyclophilin A was assessed in parallel as a loading control to which the genes of interest were normalized.
After 8 weeks of high-fat diet, blood was collected by cardiac puncture following CO2 asphyxiation into tubes containing sodium citrate anticoagulant at a ratio of 9:1. Plasma was separated by performing two centrifugation steps at 5000 rpm for ten minutes each. Prothrombin time was assessed using a Diagnostica Stago STArt 4 Hemostasis Analyzer according to the manufacturer’s protocol. Immediately before measurement, plasma was diluted 1/5 with Stago diluent buffer.
Animals were sacrificed after 8 weeks on the diet regimen and perfused with PBS. Segments of the thoracic aorta and the entire skeletal muscle were immediately frozen in TissueTek® Optimal Cutting Temperature Compound on dry ice. Ten micrometer sections were rehydrated in PBS, permeabilized with 0.3 % Triton-X-100, and incubated overnight with rabbit anti-glucose transporter GLUT4 antibody (Abcam, Cambridge, MA, ab654) at 1:50 followed by Alexa Fluor®568 goat anti-rabbit IgG (Invitrogen) at 1:800. Stained sections were mounted in fluorescence mounting medium containing DAPI. Images were collected under controlled exposure and gain settings with an Eclipse 80i (Nikon, Tokyo, Japan) with SPOT™ software (SPOT™ Imaging Solutions, Sterling Heights, MI). Six aortic and 2 skeletal muscle sections from each of 3 WT and 4 KO mice per diet group were stained for GLUT4. The mean fluorescence intensity of GLUT4 staining in the skeletal muscle was determined using ImageJ to quantify the fluorescence intensity of 20 myofibers from each mouse.
Statistical analyses of the metabolic parameters and GLUT4 quantification were performed using 2-way ANOVA followed by Bonferroni post-hoc tests. For repetitive measurements made over time (GTT, glucose, insulin), a 2-way ANOVA followed by Bonferroni post-hoc tests was performed at each time-point. The area under the curve (AUC) of the glucose tolerance test was determined using the statistical software in Graphpad. P-values for pAKT densitometry and prothrombin time were calculated using a two-tailed, unpaired t-test. Statistically significant transcriptional dysregulation after HFD was determined by applying the one sample t-test function within Acuity® to the [HFD/chow] fold-change results to calculate a p-value for each transcript. Transcripts differentially dysregulated by HFD in the WT and KO strains were determined by performing a two-tailed, unpaired t-test between the two groups.
Metabolic characterization of the galectin-3 (−/−) type II diabetic mouse model
Fasting insulin levels were consistently lower in the KO over the time course of the study until the 8 week time-point (Fig. 1c). Due to higher glucose levels and similar or lower insulin levels, homeostatic model assessment of insulin resistance (HOMA-IR) is higher in KO vs. WT on chow and HFD, but not significantly (Fig. 1d). After 5 weeks of HFD, both WT and KO mice have significantly impaired glucose tolerance compared to chow-fed controls as shown by the GTT, but this impairment is more severe in the KO (Fig. 1e, f).
Furthermore, exposure to HFD for 8 weeks does not result in a reduced response to insulin as reflected by similar levels of skeletal muscle AKT phosphorylation following an in vivo insulin challenge in both KO and WT mice on HFD (Fig. 1g, Additional file 3). However, a greater reduction in phosphorylated AKT levels was observed in aortic tissue lysates from Lgals3 (−/−) mice after HFD, indicating the onset of vascular insulin resistance (Fig. 1g). After 8 weeks of high-fat feeding, the level of circulating AGEs in the blood of HFD mice remain similar to that of chow-fed controls. Furthermore, there is no difference in AGE levels between the two strains [KO HFD: 1.8 ± 0.7 ng/mg protein; KO Chow: 1.7 ± 0.3 ng/mg protein; WT HFD: 1.7 ± 0.3 ng/mg protein; WT Chow: 1.7 ± 0.3 ng/mg protein (mean ± SD, n = 9 for HFD and n = 7 for chow)].
Endothelial cell isolation
Using this same technique and applying the same sorting gate on GFP+ endothelial cells from Tie2-GFP mice showed that approximately 75 % of GFP+ cells also display a high signal for CD105. The specificity of the endoglin antibody was also tested on C2C12 muscle cells, which showed no endoglin positive cells. This technique was much more specific than sorting based on CD31 expression and stained a greater number of GFP+ endothelial cells than TIE2 or CD146 antibodies tested. Additional files 4 and 5 show FACS profiles of the sorted endothelial population as well as negative controls and testing of endoglin specificity.
Transcriptional results and pathway analysis
Aortic endothelial responses of KO and WT mice after 8 weeks of HFD vs. chow
carbonic anhydrase 3
coagulation factor C homolog
collagen, type IV, alpha 5
epoxide hydrolase 2, cytoplasmic
tumor necrosis factor receptor superfamily, member 11b (osteoprotegerin)
prostaglandin F receptor
solute carrier 2a4 (Glut4)
thromboxane A2 receptor
adiponectin, C1Q and collagen domain containing
collagen, type XV, alpha 1
coagulation factor III
insulin receptor substrate 1
macrophage receptor with collagenous structure
protein kinase C, alpha
complement factor D (adipsin)
glycoprotein 9 (platelet)
resistin like alpha
cytochrome P450, family 2, subfamily e, polypeptide 1
matrix-remodelling associated 7
coagulation factor XIII, A1 subunit
collagen, type IV, alpha 6
insulin induced gene 1
collagen and calcium binding EGF domains 1
Skeletal muscle endothelial responses of KO and WT mice after 8 weeks of HFD vs. chow
thromboxane A2 receptor
C-type lectin domain family 1, member b
endothelin receptor type A
adiponectin, C1Q and collagen domain containing
guanylate cyclase 1, soluble, alpha 3
apolipoprotein B mRNA editing enzyme, catalytic polypeptide
glutathione S-transferase, mu 7
adenosine A2b receptor
cAMP responsive element binding protein-like 2
glutathione peroxidase 3
lymphatic vessel endothelial hyaluronan receptor 1
CASP8 and FADD-like apoptosis regulator
complement factor D (adipsin)
collagen, type XV, alpha 1
fatty acid binding protein 5, epidermal
Fc receptor, IgE, high affinity I
S100 calcium binding protein A4
interferon-induced protein with tetratricopeptide repeats 2
interferon activated gene 204
guanylate binding protein 4
endothelial PAS domain protein 1
immediate early response 2
aldehyde dehydrogenase 2, mitochondrial
high density lipoprotein (HDL) binding protein
Angiotensin I converting enzyme
Data analysis using IPA® revealed “Cardiovascular System Development and Function” as a top over-represented biological function with dysregulated transcripts presented in Additional file 6. Analysis of differentially regulated aortic endothelial transcripts between KO HFD and WT HFD mice revealed the “Coagulation Cascade” to be a highly up-regulated pathway in the KO HFD endothelium. Log2 fold change [KO HFD/WT HFD] of transcripts belonging to this canonical pathway are shown in Additional file 7.
Physiological assessment of coagulation pathway activity
GLUT4 expression in skeletal muscle and aortic endothelium
Galectin-3 (−/−) mice display greater metabolic derangement in response to HFD compared to WT mice, including both extreme hyperglycemia and impaired response to glucose challenge (Fig. 1). Our study indicates that the elevated glucose levels observed in the galectin-3 knockout may be explained by impaired glucose utilization due to reduced levels of GLUT4. Our transcriptional data revealed exacerbated down-regulation of Glut4 mRNA in the KO, by more than 2-fold in the aortic endothelium and 4-fold in the muscle endothelium compared to WT (Fig. 3). Immunofluorescence confirmed the reduced abundance of GLUT4 protein in both tissues of the KO after high-fat feeding (Figs. 5, 6).
Interestingly, chow-fed KO mice display isolated hyperglycemia, which may be useful to elucidate pathways specifically related to increased glucose. Chow-fed KO mice also show reduced abundance of GLUT4 protein relative to WT chow animals. Since GLUT4 is the primary transporter responsible for glucose uptake into insulin-sensitive tissues such as muscle and fat, reduced levels of GLUT4 likely contributes to the hyperglycemia and metabolic dysfunction observed in the KO.
The down-regulation of Glut4 expression has been reported in several models of diabetes and cardiovascular disease. It has long been known that high-fat feeding reduces GLUT4 expression in insulin-sensitive tissues, such as skeletal muscle and fat . In a rat model of type II diabetes induced by high-fat feeding and streptozotocin injection, cardiac GLUT4 protein was reduced, likely contributing to the decrease in glucose metabolism in the diabetic heart . Isoproterenol treatment has also been shown to decrease Glut4 expression, while treatment with the dipeptidyl peptidase 4 inhibitor, vildagliptin, improved Glut4 mRNA expression . In the aortae of angiotensin II hypertensive mice, GLUT4 expression was reduced by 46 % . Gaudreault et al. observed more than 50 % reduction in GLUT4 protein in en face preparations of coronary artery endothelial cells from STZ-treated rats exposed to hyperglycemia for 8 weeks . They also showed altered localization of GLUT4 from the abluminal to the luminal side of the endothelial cell derived from diabetic rats .
Studies of the Glut4 promoter have identified “high-fat responsive elements” and PPARγ regulation of transcription in adipocytes [33, 34]. In muscle, Glut4 expression is regulated by several factors including myocyte enhancer factor 2 (MEF2), GLUT4 enhancer factor (GEF), MyoD myogenic proteins, thyroid hormone receptors, and Kruppel-like factor 15 (KLF15) [35, 36]. These studies demonstrate that GLUT4 expression is subject to metabolic and tissue-specific regulation. To our knowledge, we are the first to report a link between galectin-3 and the expression of GLUT4. Further studies are necessary to understand the molecular basis of the expression of Glut4 in endothelial cells and the role of galectin-3 in the regulation of Glut4 expression.
Our studies show that galectin-3 ablation results in the down-regulation of Glut4 transcription as well as decreased abundance of GLUT4 protein. GLUT4 immunofluorescence in skeletal muscle sections appears to indicate a reduced localization of GLUT4 to the cell membrane in galectin-3 (−/−) mice compared to WT. However, further studies are necessary to determine if galectin-3 directly regulates the cell surface expression of GLUT4 and thereby affects glucose flux across the cell.
It is possible that the elevated glucose levels in the KO are due to hepatic insulin resistance, which may lead to increased gluconeogenesis and glycogenolysis. However, a recent study did not find any up-regulation of proteins involved in gluconeogenesis in the liver of the KO vs. WT mice . It is also possible that the dysregulation of other glucose transporters contributes to the observed hyperglycemia. Our transcriptional data also revealed the down-regulation of the insulin-independent glucose transporter, GLUT1, in the aortic endothelium of the KO, but to a lesser degree than GLUT4 and not significantly different from its down-regulation in WT animals. However, it is possible that its expression is reduced in other tissues, contributing to decreased glucose uptake. We did not find altered expression of GLUT1 in the muscle endothelium, but its expression in whole muscle tissue of the KO has not been investigated. Similarly, a recent study on the visceral adipose tissue of the KO did not observe differential expression of GLUT1 .
While WT mice seem to maintain lower glucose levels by compensating with increased insulin output, insulin secretion in the KO is not proportionally increased (Fig. 1c). Interestingly, pancreatic insulitis has been observed in KO mice on chow and HFD, and galectin-3 over-expression has been shown to protect B-cells against toxicity [38, 37]. It is possible that beta cell destruction or dysfunction results in reduced insulin production and/or secretion, which also affects the ability of the KO to regulate blood glucose.
By 5 weeks of HFD, both WT and KO animals display impaired glucose tolerance as demonstrated by GTT (Fig. 1e). While first phase insulin release is delayed in both strains, there is a prolonged second phase insulin response in diabetic KO animals vs. WT animals, suggesting that KO mice may be experiencing greater peripheral insulin resistance. After 8 weeks of HFD, peripheral insulin resistance in the skeletal muscle is not seen in either KO or WT mice on HFD, as demonstrated by similar levels of AKT phosphorylation compared to chow-fed controls (Fig. 1g). However, vascular insulin resistance is observed in the HFD-fed KO after this diet duration (Fig. 1g), and it is possible that muscle insulin resistance may also have earlier onset in the KO.
Interestingly, circulating levels of advanced glycation endproducts (AGEs) are similar between both strains and are not elevated by 8 weeks of high-fat diet. Therefore, the differential responses we observe between the KO and WT are likely to be independent of galectin-3’s role in the uptake of AGEs. Previous studies have attributed the protective effects of galectin-3 in diabetes and atherosclerosis to an indirect role in the removal of AGEs from circulation, thereby preventing subsequent tissue damage and activation of the pro-inflammatory RAGE-pathway [14, 39]. Our data suggests a direct involvement of galectin-3 in the regulation of glucose metabolism and vascular pathology.
Our assessment of the endothelial response to diabetes in KO and WT mice has shown a greater transcriptional response in the aortic endothelium of KO mice, with twice as many dysregulated transcripts compared to WT. Transcripts with recognized roles in metabolic signaling, ECM synthesis, vasoconstriction, coagulation, and inflammation are more dysregulated in the aortic endothelium of the KO compared to WT (Fig. 3, Table 1). For example, the glucose transporter, Glut4, is down-regulated by HFD in both strains, but its reduction in the KO is twice as great as WT (Fig. 3). Similarly, expression of Irs-1, insulin-induced gene 1, Igf1, and glucose-6 phosphatase are dysregulated to a greater degree in the KO vs. WT on HFD (Fig. 3). The exacerbated response of the KO aortic endothelium to HFD suggests a greater derangement of metabolic signaling in these cells and implicates LGALS3 in the preservation of such pathways.
In the capillary endothelium of the skeletal muscle, there was less dysregulation by HFD in both strains compared to the aortic transcriptional response, suggesting a greater role for galectin-3 in the macrovascular complications of diabetes compared to microvascular disease. However, transcripts with roles in glucose homeostasis, atherosclerosis, and matrix remodeling are differentially regulated by HFD in the KO microvasculature vs. WT (Table 2). Transcripts which serve a protective role in glucose homeostasis, such as Glut4 and the adenosine A2 receptor, are preferentially down-regulated. Adenosine A2 receptor knockout mice exhibit impaired glucose tolerance and insulin signaling .
Several ECM components and matrix remodeling proteins are also dysregulated in the diabetic KO endothelium (Fig. 3, Tables 1 and 2). In the muscle endothelium, a receptor for hyaluronic acid (HA), Lyve1, is downregulated 6-log2 fold (Fig. 3). Lyve1 is expressed in lymphatic endothelial cells and is involved in the degradation of HA, a matrix component that facilitates cell migration during wound healing and inflammation . The downregulation of Lyve1 suggests decreased HA turnover which could impair wound healing in the diabetic KO.
Transcripts implicated in atherosclerosis are also dysregulated in the KO endothelium. HDL binding protein, which is normally expressed by endothelial cells, is down-regulated by HFD in the WT but not the KO. This protein was up-regulated in atherosclerotic lesions in human coronary arteries . Similarly, transcriptional up-regulation of the scavenger receptor, Marco, in the aortic endothelium of the diabetic KO by more than 4-log2 fold over chow-fed controls was observed by array and qPCR (Fig. 3). This receptor serves a role in the binding and uptake of modified LDL and has been shown to be constitutively expressed in endothelial cells of the lymph nodes .
Pathway analysis of the HFD-fed KO and HFD-fed WT transcriptional profiles revealed the up-regulation of transcripts involved in the coagulation cascade in the KO endothelium (Additional file 7). Our physiological assessment of coagulation activity confirmed that diabetic KO mice exhibit reduced prothrombin time compared to diabetic WT mice and chow-fed controls (Fig. 4). Prothrombin time specifically measures activation of the extrinsic coagulation pathway activated by tissue factor. Therefore, the diabetic KO displays increased activation of the extrinsic coagulation pathway, which has important implications for diabetic patients where increased activation of the coagulation cascade could promote thrombotic complications.
Furthermore, the transcript for von Willebrand factor (vWF), a protein important in platelet activation and aggregation, is also up-regulated in the endothelium of HFD KO mice compared to HFD WT mice (Additional file 7). Recent studies have shown that LGALS3 and LGALS1 interact directly with vWF in endothelial cells and in plasma via the N-linked glycans of vWF . The inhibition of these galectins was associated with increased vWF-platelet string formation and more rapid thombus formation after injury . Our data suggests that LGALS3 may protect against thrombus formation in diabetes by suppressing vWF expression in the endothelium.
Our analysis of the transcriptional response of the endothelium to an in vivo model of type II diabetes in galectin-3 deficient vs. wild-type mice has revealed differential responses in both aortic and skeletal muscle tissues. The KO displays altered expression of transcripts with roles in the glucose-insulin signaling pathway, ECM composition, vasoregulation, redox homeostasis, inflammation, coagulation, atherosclerosis, and endothelial dysfunction. Down-regulation of GLUT4 mRNA and protein in the endothelium and muscle of galectin-3 (−/−) mice may explain the hyperglycemia experienced by these mice, and suggests a role for galectin-3 in the regulation of glucose uptake. Transcriptional up-regulation of coagulation and pro-thrombotic factors and increased blood clotting activity in the plasma from diabetic KO mice also suggests a protective role for galectin-3 in mediating coagulation and thrombosis. The transcriptional findings, as well as the altered metabolism demonstrated by KO mice compared to WT mice, suggest that galectin-3 serves a protective role against the metabolic and hormonal derangements that impinge upon the diabetic endothelium and lead to its damage and dysfunction.
This work was supported by American Heart Association Predoctoral Award 11PRE7720065 (to A.L. Darrow) and National Institute of Health grants RR-016453 and HL-073449 (both to R.V. Shohet).
We would like to thank the Molecular and Cellular Immunology Core supported by RCMI (G12 MD007601), the Genomics Core supported by the Cardiovascular COBRE (P20 GM103341), and the Histology and Imaging Core supported by both grants. We are grateful to Dr. James Padbury and his lab at Women and Infants Hospital (Providence, RI) supported by COBRE P20GM103537 for their guidance and the generous use of their facilities.
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