Fig. 1

Endocrine Responses of KO vs. WT mice fed a high-fat diet. (a) Weight after 8 weeks of diet (HFD n = 19-23; Chow n = 16-17). Increase in fasting glucose (b) and insulin (c) levels over the time course of the diet regimen (HFD n = 7-26; Chow n = 4-22). (d) HOMA-IR calculated from fasting glucose and insulin levels after 8 weeks of diet (n = 9-10). (e) Glucose tolerance test performed by measuring glucose levels every 20 min after i.p. injection of glucose (1 mg/kg, n = 8-9) and (f) corresponding area under the glucose tolerance curve. (g) Sensitivity of aortic and skeletal muscle tissues to insulin challenge (60 U/kg) after 8 weeks of high-fat or chow diet measured by western blotting for phosphorylated AKT. Densitometry was performed using Image J, and pAKT levels are expressed as a percentage of total AKT levels (n = 3-8). Data is shown as mean ± SEM. *P < 0.05;**P < 0.01; ***P < 0.001; ^† P < 0.05 for the interaction of diet and genotype determined by 2-way ANOVA