Clinical usefulness of lipid ratios, visceral adiposity indicators, and the triglycerides and glucose index as risk markers of insulin resistance

Cardiovascular disease (CVD) is now the leading cause of morbidity and mortality worldwide. The new epidemic of diabetes is fuelling an alarming increase in premature CVD incidence. Insulin resistance (IR) and the consequence of compensatory hyperinsulinemia are fundamentally pathogenetic factors for a set of metabolic abnormalities [1], which contribute to the development of diabetes and CVD. Thus, detection and treatment of IR before the manifestation of clinical disease are of paramount importance. Although hyperinsulinemic-euglycemic clamp is the gold standard test for measurement of IR, it is impractical in clinical settings due to practical, ethical and economic reasons. Therefore, markers of IR which are simple and easy to implement are urgently needed. Dyslipidemia is a major risk factor for CVD. There is evidence suggesting that traditional lipid ratios, such as total cholesterol (TC)⁄ high density lipoprotein cholesterol (HDL-C), non-HDL-C/HDL-C, and triglycerides (TG)/HDL-C, which take account of the proportion between the pro-atherogenic and anti-antherogenic fractions, are more effective than single measures of lipids in detecting IR [2]. A plethora of studies have shown that apolipoprotein B (apoB)/apolipoprotein A1 (apoA-I) (in which apo B means apo B100) ratio is superior to traditional lipids in CVD prediction [3]-[5]. Moreover, one study indicates that apoB/apoA-I is significantly associated with IR [6]. These analyses have fuelled the recommendation that apoB and apoA-I should be measured in routine clinical care [7]. It is uncertain, however, whether the apoB/apoA-I ratio is superior to traditional lipid ratios in detecting IR. Though head-to head comparisons between apoB/apoA-I and traditional lipid ratios have been conducted to evaluate whether apoB/apoA-I could be used instead of traditional lipid ratios for CVD risk [8], direct comparisons between these measurements to identify IR are limited.

On the other hand, emerging evidence indicates that visceral adiposity is associated with IR [9],[10]. The visceral adiposity index (VAI), a mathematical model that uses both anthropometric (body mass index [BMI] and waist circumference [WC]) and metabolic (TG and HDL-C) parameters, and lipid accumulation product (LAP), a mathematical model that based on a combination of TG and WC, are sensitive markers of visceral obesity [11],[12], and have the ability to identify IR. Since both the VAI and LAP incorporates TG along with indicators of adiposity (WC), they might be superior markers of IR compared with lipid ratios, as regional fat distribution can modulate cardiometabolic risk [9],[13]. It has also been suggested that the product of TG and fasting glucose (TyG) has high sensitivity for recognizing IR [14],[15]. Since TyG incorporates TG along with fasting plasma glucose (FPG), superiority of TyG in identifying IR might be achieved as both TG and FPG are well validated for their roles in IR [16],[17]. To the best of our knowledge, limited data are available regarding direct comparisons between the variables mentioned above in identifying IR.

Hence, the aims of this study were to: 1) evaluate the performance of a range of lipids and apolipoproteins, as well as relevant ratios in identifying IR; 2) directly compare the traditional lipid ratios, apoB/apoA-I ratio, VAI, LAP, and TyG for strength and independence as risk factors for IR.

Results were remarkably similar when IR was defined by HOMA2-IR as greater than the 75th percentile, which was calculated using the HOMA2 calculator updated in 2007 (data not shown).

In the present study, we directly compared lipid and apolipoprotein measures, lipid ratios, LAP, VAI, and TyG as predictors of IR. Overall, we observed that lipid ratios were superior to either pro-atherogenic or anti-atherogenic fractions alone. The apoB/apoA-I performed no better than any of the traditional lipid ratios. Of the lipid ratios, the magnitude of the association was greatest for TG/HDL-C. TyG appears to be most closely associated with IR among all the variables studied. VAI and TG/HDL-C were equivalent in their relationships with IR. Moreover, ROC curve analysis confirmed these observations by indicating that TG/HDL-C was better than apoB/apoA-I in discriminating IR and TyG was the best discriminator in identifying IR.

There have been several reports suggest that traditional lipid ratios have greater predictive power for IR and CVD risk than each of the single standard lipid measures [2],[22],[23]. However, there has been very few study address the crucial question of whether apoB/apoA-I predicts IR better than traditional lipid ratios do. Plasma apoB concentration provides a measure of the concentration of all potentially atherogenic particles (Each very low-density lipoprotein, intermediate-density lipoprotein, and LDL particle is covered by 1 apoB molecule), and increased small dense LDL particles is related with IR and CVD risk. On the other hand, apoA-I is the main structural protein of HDL particles. Consequently, apoB/apoA-I has been recommended as an effective indicator to assess CVD risk [7]. However, the question of whether apoB/apoA-I should be introduced into guidelines as a target for therapy in people with dyslipidemia is a debated issue, as some reports indicated that apoB/apoA-I were better predictors of CVD risk compared with traditional lipid ratios [22],[23], and other studies reported that apoB/apoA-I and non-HDL-C/HDL-C were comparable in terms of predicting CVD risk [8],[24]. Accumulating evidence indicates that IR facilitates the development of CVD. However, data on direct comparisons between traditional lipid ratios and apoB/apoA-I to identify IR is limited. Despite the evidence that apoB/apoAI ratio is significantly associated with IR [6], we found apoB/apoA1apoA-I has little to offer over traditional lipid ratios (TC/HDL-C, LDL-C/HDL-C, and non-HDL-C/HDL-C) in predicting IR. One possible explanation for this result may be that 90% of apoB-carrying particles are found on LDL, irrespective of their size. It has been indicated that LDL-C has little correlation with IR. Moreover, the OR for TG/HDL-C was greater than that for apoB/apoA-I. Furthermore, evidence shows that two simple algorithms to calculate apoB from routine lipids are effective in estimating measured apoB [25].Taken together, these results provide little support for replacing measurement of traditional lipid ratios with measurement of the apoB/apoA-I ratio as risk prediction tools. This information is of pragmatic interest, as traditional lipid ratios can be directly calculated from traditional lipid measures at no incremental cost.

Although lipoproteins and apolipoproteins were not measured by the more sophisticated method nuclear magnetic resonance spectroscopy, emerging evidence suggested that the association of coronary artery calcification with nuclear magnetic resonance-measured lipoproteins was comparable to that with standard lipids [26]. It has been reported that the sensitivity and specificity for TG/HDL-C in identifying IR are virtually identical to those for fasting insulin concentration [27]. Furthermore, TG/HDL-C has been shown to have a stronger correlation with IR when compared with other traditional lipid or lipid ratios [28],[29]. However, correlations between TG/HDL-C and IR differ according to ethnic origin [30]. Thus, it is possible that how well TG/HDL-C correlates with IR depends on the target population. Our study appears to be the first Chinese population-based study using a large database of cohort for exploring whether TG/HDL-C is a better correlate of IR compared with other lipid measures. Consistent with other studies [28],[29],[31], we found TG/HDL-C was a better marker for IR than any of other lipid parameters. Given that standard assay for TG and HDL-C are widely available in clinical practice, and that it is simple to calculate TG/HDL-C from TG and HDL-C, as well as that TG/HDL-C is superior to other lipid parameters in predicting IR, it is reasonable to recommend TG/HDL-C as an effective and convenient indicator of IR.

Since the concurrence of hypertriglyceridemia and low HDL-C are key metabolic abnormalities in patients with IR and represent diabetic dyslipidemia, it is expected that we found a superiority of TG/HDL-C over other lipid measures in correlating IR. However, it is worth emphasizing that variations in several parameters (levels of lecithin:cholesterol acyltransferase, post-heparin hepatic lipase activities, post-heparin lipoprotein lipase activities, phospholipid transfer protein) that control the plasma lipoprotein metabolism are related to an increased degree of insulin sensitivity among healthy (BMI <30 Kg/m²) high HDL-C (>60 mg/dL) subjects [32]. Binding and internalization rates of TG-enriched HDL in these subjects may slow down [33], inducing a relatively lower level of IR.

Our study is the only one directly comparing the utility of VAI with different lipid measures in assessing IR risk. We found that the VAI, which take account of the concentrations of TG and HDL-C, as well as adiposity measures (BMI and WC), and TG/HDL-C have comparable associations with IR. However, when we used ROC analysis to determine the ability of these 2 indices to discriminate between persons with and without IR, we found that VAI was a better discriminator, indicating that VAI offers more clinical information than that achieved by using TG/HDL-C. The better ability of VAI to identify IR individuals compared with TG/HDL-C is of clinical relevance and can be possibly explained by the fact that the VAI takes regional body fat distribution into consideration. VAI is a good indicator of visceral adiposity [11] and visceral fat is more metabolically deleterious than subcutaneous fat [13],[34]. According to the overflow hypothesis, the subcutaneous fat depot appears to act as a protective metabolic sink, storing dietary fat to limit their deposition in undesired sites such as liver, heart, skeletal muscle, and pancreatic β cells [34]. However, for some individuals with more visceral fat and less subcutaneous fat, available lipids exceed the subcutaneous adipose tissue’s capacity for buffering and storage, the excess of lipids will be reoriented toward nonadipose tissues [35]. The ectopic lipid deposits lead to lipotoxicities, incurring insulin resistance. Hence, obesity is a remarkably heterogeneous disorder, as evidenced by the occurrence of a subset of obese persons who are insulin sensitive, and a subset of normal-weight people who are insulin resistant [36]. It is with the heterogeneity in mind that measuring an indicator of visceral fat such as VAI is clinically benefit, as visceral obesity is more important in modulating IR [9],[13]. In the current study, LAP, another indicator of visceral adiposity, does not offer any clinical benefit as compared with TG/HDL-C. Although explanations for this issue remain to be elucidated, it is probably related to the fact that LAP does not incorporate anti-atherogenic lipoproteins.

TyG has been recently recommended as a simple and inexpensive index to evaluate IR [14],[15]. Because of the variability of TG levels according to ethnicity, it is necessary to assess the utility of TyG in predicting IR in a Chinese population. The current analysis was initiated to perform the task. We found that the TyG index was a good discriminator in predicting IR and was a better correlate of IR than TG/HDL-C, a finding consistent with results from other studies [14],[15]. Moreover, our study extends previous studies by directly comparing the utility of TyG with visceral adiposity indicators and other lipid parameters in assessing IR risk. We found that TyG was the best index in discriminating IR individuals, highlighting that TyG can serve as a simple index for identifying individuals with a high risk of IR. The superiority of TyG to any of the other indices we studied can be possibly explained by the fact that glucotoxicity and lipotoxicities are key mechanisms in modulation of IR. A recent study indicated that 20/(fasting C-peptide immunoreactivity × fasting glucose) outperformed HOMA-IR for the assessment of glucose infusion rate measured with the hyperinsulinemic-euglycemic glucose clamp [37]. Since fasting C-peptide immunoreactivity was not available in CHNS 2009, we have no way to validate the usefulness of TyG in identifying IR evaluated with 20/(fasting C-peptide immunoreactivity × FPG), and further research with data of fasting C-peptide immunoreactivity is warranted.

Our study has several strengths including a well-established cohort of Chinese population, a vigorous quality assurance program and the same strict methodology used to ensure the quality of the data collection over the entire study period. In addition, we evaluated the performance of traditional lipid ratios, apo B/apo A-I ratio, VAI, LAP, and TyG in correlating with IR across the full glycemic spectrum (from normal glucose tolerance to impaired glucose tolerance to diabetes). Nevertheless, the present study has several limitations. The study population is comprised of only Chinese adults, thus, extrapolating results to other racial or ethnic population should be interpreted cautiously. The cross-sectional design implies that no potential temporal relations can be drawn. Hyperinsulinemic-euglycemic clamp, the gold standard for evaluating insulin sensitivity, was not conducted. However, HOMA-IR, a surrogate of IR, has been shown to correlate well with IR index derived from the euglycemic clamp [38]. The fact that we have no way to evaluate the associations of TG/HDL-C and apoB/apoA-I ratio with IR according to LDL subfraction phenotype is another limitation, as phenotype B is associated with increased oxidized LDL, glycated LDL, and lipoprotein-associated phospholipase A2 (also known as platelet-activating factor acetylhydrolase) activity [39] and with increased CVD risk [40].

Apolipoprotein ratios performed no better than any traditional lipid ratio in predicting IR risk. The TG/HDL-C ratio, VAI and TyG index are effective discriminators in predicting IR. This information is of particular clinical relevance, as all these 3 indices can be calculated from common measures such as TG, FPG, and HDL-C, which are based on standardized measurements and widely available. The ability of TG/HDL-C to identify IR seems compromised by taking account of body fat distribution. The TyG index is the best marker predicting the risk of IR.

TTD conceived the study design, wrote the first draft of the manuscript, analyzed the data, contributed to interpretation of results, commented on drafts, and approved the final version. GY, MXZ, XRZ, and ZLL contributed to interpretation of results, commented on drafts, and approved the final version. XXS and XFY are the guarantors of this work, and had full access to all the data in the study and takes responsibility for the integrity of the data and the accuracy of the data analysis. All authors read and approved the final manuscript.