Cardiovascular Diabetology

Table 2 QPCR analysis of selected miRNAs in arterial FFPE samples from T2DM and Non-DM patients

From: No detectable differential microRNA expression between non-atherosclerotic arteries of type 2 diabetic patients (treated or untreated with metformin) and non-diabetic patients

microRNA	Assay nr.	References	T2DM n = 26	Non-DM n = 26	P	T2DM (− Met) n = 11	Non-DM n = 26	P	T2DM (+ Met) n = 15	T2DM (− Met) n = 11	P
microRNA	Assay nr.	References	Mean ± SD	Mean ± SD	P	Mean ± SD	Mean ± SD	P	Mean ± SD	Mean ± SD	P
miR-21-5p	397	[37]	0.112 ± 0.079	0.101 ± 0.098	0.928	− 0.012 ± 0.098	0.101 ± 0.098	0.493	0.204 ± 0.117	− 0.012 ± 0.090	0.183
miR-24-3p	402	[29, 30]	0.123 ± 0.093	0.100 ± 0.114	0.876	− 0.099 ± 0.114	0.100 ± 0.114	0.303	0.286 ± 0.126	− 0.099 ± 0.114	0.039
miR-26a-5p	405	[31]	0.120 ± 0.071	0.116 ± 0.096	0.978	− 0.018 ± 0.096	0.116 ± 0.096	0.408	0.220 ± 0.095	− 0.018 ± 0.095	0.097
miR-182-3p	483	[32]	1.215 ± 0.264 (n = 10)	1.449 ± 0.179 (n = 17)	0.456	1.037 ± 0.179 (n = 5)	1.449 ± 0.179 (n = 17)	0.326	1.393 ± 0.297 (n = 5)	1.037 ± 0.457 (n = 5)	0.532
miR-145-5p	2278	[33, 34]	0.134 ± 0.088	0.107 ± 0.109	0.846	− 0.067 ± 0.109	0.107 ± 0.109	0.346	0.281 ± 0.121	− 0.067 ± 0.103	0.047
miR-29a-3p	2112	[12,13,14,15,16,17]	0.158 ± 0.075	0.180 ± 0.084	0.849	0.064 ± 0.084	0.180 ± 0.084	0.434	0.227 ± 0.105	0.064 ± 0.104	0.294
miR-29b-3p	413	[12,13,14,15,16,17]	0.150 ± 0.091	0.220 ± 0.087	0.582	0.017 ± 0.087	0.220 ± 0.087	0.240	0.247 ± 0.102	0.017 ± 0.160	0.216
miR-221-5p	2096	[28]	NA	NA	…	NA	NA	…	NA	NA	…
miR-222-5p	2097	[28]	0.246 ± 0.118 (n = 25)	0.306 ± 0.084	0.674	− 0.135 ± 0.084 (n = 10)	0.306 ± 0.084	0.011	0.499 ± 0.134	− 0.135 ± 0.154 (n = 10)	0.006
miR-132-3p	457	[35]	0.123 ± 0.090	0.189 ± 0.105	0.636	− 0.040 ± 0.105	0.189 ± 0.105	0.231	0.242 ± 0.108	− 0.040 ± 0.144	0.122
miR-212-3p	515	[35]	− 0.072 ± 0.043	− 0.013 ± 0.075	0.501	− 0.122 ± 0.075	− 0.013 ± 0.075	0.358	− 0.035 ± 0.072	− 0.122 ± 0.028	0.326
miR-1298-5p	2861	[36]	− 0.099 ± 0.041	0.035 ± 0.070	0.104	− 0.222 ± 0.070	0.035 ± 0.070	0.031	− 0.009 ± 0.047	− 0.222 ± 0.054	0.007

For each of the 12 miRNAs three patient group comparisons were made: T2DM vs. non-DM patients; non-metformin-treated T2DM (− Met) vs. non-DM patients; and non-metformin-treated T2DM (− Met) vs. metformin-treated T2DM patients (+ Met). Normalization was performed using equal amounts of total RNA for each sample and by normalizing the relative expression to the synthetic miRNA ath-miR-159a. Values are mean ± SD. Assay nr. refers to TaqMan microRNA assays (Life Technologies)

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