Fig. 5
HDAC inhibition decreases HDAC activity and increases GLUT1 and GLUT4. a Measurement of HDAC activity in diabetic myocardium from different groups; b representative Western blot of HDAC4 proteins in myocardium; c densitometric analysis of Western blot of HDAC4 protein levels; d representative Western blot of GLUT4 proteins in myocardiums; e densitometric analysis of GLUT4 proteins in myocardium; f representative Western blot of GLUT1 proteins in myocardium; g densitometric analysis of GLUT1 proteins in myocardium; h GLUT1 acetylation in diabetic myocardium. IB immunoblot; IP immunoprecipitation; IgG serves as IP control; lysates from myocardium serve as signal control of detected proteins. The densitometric signal was normalized to the control group and expressed as a percentage. Values are shown as mean ± SEM (n = 3 per group); *p < 0.05 vs CTRL, # p < 0.05 vs STZ+ NaBu. NaBu sodium butyrate.