Figure 4
End diastolic (ED) interfilament (d1,0) spacing (a) and systolic interfilament spacing (b) from experiment 2. In comparison to control rats, ED and peak systolic d1,0 spacing in the was not significantly different in the epicardium between the groups. In the subepicardium, diabetic rats had a significantly smaller ED and systolic d1,0 spacing compared to control rats (P < 0.05). However, in the subendocardial layer, both the diabetic and diabetic rats treated with fasudil (10 mg/kg/day) had significantly reduced ED d1,0 spacing (P < 0.05) and systolic spacing (P < 0.01, P < 0.05, respectively) in comparison to control rats. Data is expressed as mean ± SEM. *P < 0.05 and **P < 0.01 vs. control in the same myocardial layer. N = 4–6 per group.