Insulin and/or Heparin Protected PAECs from High Glucose Injury when bFGF was Present in Cell Medium. PAECs were treated with glucose (30 mM), glucose plus bFGF (1 ng/ml), glucose plus bFGF plus insulin (1 U/ml), glucose plus bFGF plus heparin (0.5 μ g/ml) and glucose plus bFGF plus insulin plus heparin for seven days. Cell medium was changed and fresh reagents were added every other day. Cells were counted and medium LDH was determined 48 hrs after the last addition of reagents. Results are expressed as mean +/- SE of three dishes per group. Significantly different than a, control; b, glucose; c, glucose+bFGF; d, glucose+bFGF+heparin (P < 0.05) (one-way ANOVA).