Table 1 Primers for RT-PCR reaction
Gene title | Accession number | Primer sequence | Cycle | Ta (°C) | Product (bp) |
|---|---|---|---|---|---|
Chemerin | NM_002889.3 | F: 5'-GAAGAAACCCGAGTGCAAAG-3' | 28 | 56 | 229 |
| Â | Â | R: 5'-CTTGGAGAAGGCGAACTGTC-3' | Â | Â | Â |
ChemR23 | NM_001142343.1 | F: 5'-CTCCCAATCCATATCACCTA-3' | 32 | 58 | 543 |
| Â | Â | R: 5'-GCAGAGGAAGAAGGTAATGA-3' | Â | Â | Â |
Adiponectin | NM_001177800.1 | F: 5'-CTCCTCCTCACTTCCATTCTG-3' | 35 | 58 | 310 |
| Â | Â | R: 5'-TTTCACCGATGTCTCCCTTA-3' | Â | Â | Â |
TNF-α | NM_000594.2 | F: 5'-TTCTGCCTGCTGCACTTTGGA-3' | 40 | 60 | 592 |
| Â | Â | R: 5'-GGCGTTTGGGAAGGTTGGATG-3' | Â | Â | Â |
β-actin | NM_001101.3 | F: 5'-AGGTCATCACCATTGGCAAT-3' | 26 | 58 | 357 |
| Â | Â | R: 5'-ACTCGTCATACTCCTGCTTG-3' | Â | Â | Â |