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Table 1 Primers for RT-PCR reaction

From: Association of chemerin mRNA expression in human epicardial adipose tissue with coronary atherosclerosis

Gene title Accession number Primer sequence Cycle Ta (°C) Product (bp)
Chemerin NM_002889.3 F: 5'-GAAGAAACCCGAGTGCAAAG-3' 28 56 229
   R: 5'-CTTGGAGAAGGCGAACTGTC-3'    
ChemR23 NM_001142343.1 F: 5'-CTCCCAATCCATATCACCTA-3' 32 58 543
   R: 5'-GCAGAGGAAGAAGGTAATGA-3'    
Adiponectin NM_001177800.1 F: 5'-CTCCTCCTCACTTCCATTCTG-3' 35 58 310
   R: 5'-TTTCACCGATGTCTCCCTTA-3'    
TNF-α NM_000594.2 F: 5'-TTCTGCCTGCTGCACTTTGGA-3' 40 60 592
   R: 5'-GGCGTTTGGGAAGGTTGGATG-3'    
β-actin NM_001101.3 F: 5'-AGGTCATCACCATTGGCAAT-3' 26 58 357
   R: 5'-ACTCGTCATACTCCTGCTTG-3'    
  1. Abbreviations: TNF-α, tumor necrosis factor-α; Ta, annealing temperature.