Fig. 4

Subpopulation analysis of endothelial cells. A. UMAP plot of endothelial cells re-clustering into four subpopulations. B. Bar plot of annotated subpopulations of endothelial cells for each sample. C. Volcano plot of the fold-change in differential gene expression in WT and DCM groups. Wilcoxon rank-sum test was used to calculate P-values and the Bonferroni analysis was used to calculate adjusted P values. D. Violin plot of biological processes scores defined as average z-scores of process-related genes (right) for selected subpopulations. Significance was determined by the Wilcoxon test and divided into three levels (NS, not significant (P > 0.05); *** P < 0.001; **** P < 0.0001). E. Bar plot of GO enrichment terms of differentially expressed genes in cluster 0 of endothelial cells. F. Bar plot of GO enrichment terms of differentially expressed genes in cluster 1 of endothelial cells. G. Overview of ligand-receptor interactions between fibroblast and other cell types; circle size represents P-values, scale on the right. The color scale represents the means of the average expression level of ligands and receptors in the corresponding cluster. H. Overview of ligand-receptor interactions between endothelial cells and other cell types; circle size represents P-values, scale on the right. The color scale represents the means of the average expression level of ligands and receptors in the corresponding cluster