Fig. 9

Circ_005077 promoted ferroptosis in H9c2 cells via p47phox. H9c2 cells (A∼F, O) and primary neonatal rat ventricular cardiomyocytes (NRVCs) (G, M, N) were transfected with NC;Si-p47phox;SH-circ_005077;SH-circ_005077 + vector (co-transfected with SH-circ_005077 and vector); or SH-circ_005077 + OE-p47phox(co-transfected with SH-circ_005077 and OE-p47phox). (A) Microbiology of mitochondria under electron microscope (1,500×, upper row, and magnified views at 6,000, lower row). (B) The expression levels of GSH. (C) The MDA expression levels. (D, H) Ferrous iron representative photos and quantitative analysis (orange, 400× magnification). (E, I) LPO representative images and quantitative analysis (green, 400× magnification). (F, J, K) MitoROS (red, 400× magnification) and cellular ROS (green, 400× magnification) representative photos and quantitative analysis. (G, L) F-actin representative images (red,600× magnification) and quantitative analysis of cell surface area. (M) Rescue experiments detect the ANP mRNA expression levels in NRVCs by the qPCR assay. (N) Rescue experiments detect the BNP mRNA expression levels in NRVCs by the qPCR assay. (O) Western blotting detecting TFRC, ACSL4, COX2, FTH1, and GPX4 protein levels. Data were presented as the normalized mean ± SD of at least three independent experiments. *P < 0.05; **P < 0.01; ***P < 0.001. NS, no significance