Fig. 4

Circ_005077 acts as a regulator for palmitate PA-induced ferroptosis in cardiomyocyte. H9c2 cells were transfected with OE-circ_005077 mediated by lentivirus (10MOI), vector, SH-circ_005077 (10MOI), and NC, respectively (C–I), followed by 250 µM PA treatment for 24 h. (A) Representative pictures of H9c2 mitochondria structure under a transmission electron microscope (1,500×, upper row and magnified views at 6,000, lower row). (B, E) Illustrations and quantitative analysis of ferrous iron (orange, 400× magnification). (C, F) Illustrations and quantitative analysis of LPO (green, 400× magnification). (D,G,H) Illustrations and quantitative analyses of mitoROS (red, 400× magnification) and cellular ROS (green, 400× magnification). (I) Intracellular GSH content. (J) Intracellular MDA content. (K)Protein levels of TFRC, ACSL4, COX2, FTH1, and GPX4 in treated H9C2 detected via Western blotting. Each point showed the average fluorescence intensity of a single experiment. Data were shown as mean ± SD of at least three independent experiments. *P < 0.05; **P < 0.01; ***P < 0.001