Fig. 3

Circ_005077 is involved in the ferroptosis signal pathway. H9c2 cells were transfected with lentivirus-mediated OE-circ_005077 and vector. (A) The transcriptomes of the RNA samples were profiled by RNA sequencing (RNA-Seq). Cluster analysis of differential genes (DEGs) between the OE_circ_005077 and vector groups. Heat map taken from the top 100 genes with the lowest p-value. Red indicates high gene expression and blue indicates low gene expression. (B) The findings of the KEGG pathways of DEGs, GO biological process enrichment, GO cellular component enrichment, and GO molecular function enrichment. H9c2 cells were treated with erastin (5 µM) or PA (250 µM), with or without of Fer-1(2 µM) for 24 h (C–E). (C, D) The CCK-8 assay was used to determine cell viability. (E) The morphology of the H9c2 was observed using a light microscope. The results of six biological replicates were displayed using scatter plots. Data were presented as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001