Fig. 2

Circ_005077 regulates palmitate (PA)-induced myocardial lipotoxicity in vitro. H9c2 cells or primary neonatal rat ventricular cardiomyocytes (NRVCs) were transfected with OE-circ_005077 mediated by lentivirus (10MOI), vector, SH-circ_005077 (10MOI)), and NC respectively. (C–I) The H9c2 were then given a 24 h treatment with 250 µM PA and the NRVCs were followed by treatment with 150 µM PA for 48 h (E–I). (A) Oil-red O staining exhibited lipid deposition in PA-treated H9c2 cells (400× magnification). (B) The abundance of circ_005077 in H9c2 cells treated with 250 µM PA at the defined time points was determined by qRT-PCR compared to the treatment of BSA (n = 3). (C) Circ_005077 overexpression efficacy of OE-circ_005077 compared to vector in H9c2. (D) Circ_005077 knocks down the efficacy of SH-circ_005077 compared to NC in H9c2. (E) LDH released from H9c2 was measured via colorimetry. (F and G) Immunofluorescence (IF) of NRVCs for F-actin (red, 600× magnification) and DAPI (blue, 600× magnification). Following IF, cell surface area was measured using morphometry (each dot represents the mean area of a single experiment). (H and I) Analysis of the relative expression of ANP and BNP mRNA in NRVCs. The results of three to six biological replicates were displayed using scatter plots. The data were presented as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001, NS, no significance