Fig. 6

TZT Effects on apoptosis in AC16 cells exposed to high glucose A Apoptosis was measured using Annexin V-FITC staining followed by a flow cytometer. The histogram represents the median of fluorescence (FITC). mRNA expression and protein levels of pro-apoptotic B BAX, anti-apoptotic C Bcl2 and analysis of D BAX/Bcl2 ratio at the protein level. Also, mRNA expression and protein levels of E Total and cleaved form of caspase-3 exposure to normal glucose (NG), high glucose (HG), or HG with 100 nM TZT were shown. The fold increase of mRNA expression compared with NG was calculated using the 2^−ΔΔCt method. Protein expression densitometry analysis was performed using Image J 1.52n software. Data are mean ± SEM of three independent experiments. *p < vs NG; ** p < vs HG