Fig. 4
From: Evidence that tirzepatide protects against diabetes-related cardiac damages
Effects of TZT on calcium homeostasis markers A SERCA2 mRNA expression, total protein and phospho-T484 protein levels B PLN mRNA expression, total protein and phospho-S16 and -T17 of PLN. C CAMKII mRNA expression, total form of CAMKII and phospho-T287 D mRNA expression and protein levels of PKA exposure to NG, HG, or HG with 100 nM TZT after 7 days. β-Actin was used as internal control for gene expression. The fold increase of mRNA expression compared with NG was calculated using the 2−ΔΔCt method. Protein expression densitometry analysis was performed using Image J 1.52n software. Data are mean ± SEM of three independent experiments. *p < vs NG; ** p < vs HG