Fig. 5
Targeting EZH2-H3K27me3 epigenetic signature rescues abnormal phenotypes in endothelial cells isolated from patients with diabetes. A Representative confocal microscopy images of H3K27me3 (green), EZH2 (red), and EZH2/H3K27me3 colocalization (yellow) and densitometric quantification of fluorescence intensity. Cell nuclei are stained with Hoechst (blue). Scale bar = 2 μm. n = 6/group. B and C RT-qPCR arrays showing gene expression of SOD1, SOD2, JunD, and C NOX4, respectively (n = 3/group). D electron spin resonance (ESR) spectroscopy analysis of O2− production (n = 3/group). E NF-κB p65 binding activity (n = 6/group), and F gene expression of IL-6 and MCP-1 (n = 3/group). IL-6, interleukin-6; MCP-1, monocyte chemoattractant factor-1