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Fig. 7 | Cardiovascular Diabetology

Fig. 7

From: Persistent epigenetic signals propel a senescence-associated secretory phenotype and trained innate immunity in CD34+ hematopoietic stem cells from diabetic patients

Fig. 7

Phenotypical characterization of CAD ± T2DM-CD34+ HSPC-derived monocytes. A analysis of the total monocytes (CD14+) generated in vitro from CAD ± T2DM-CD34+ cells after myeloid differentiation and (B) of their intermediate (CD14hiCD16+) and (C) classical (CD14hiCD16) subpopulations by flow cytometry. The data are expressed as FC (CAD vs CAD-T2DM; unpaired t-test). D Flow cytometric dot blot analysis of a representative differentiation experiment. E and F p21 and p27 gene expression analysis by qPCR (2−ΔΔCt) and G quantification of proinflammatory cytokines released in the medium after LPS stimulation. The data are expressed as FC (CAD vs CAD-T2DM; unpaired t-test). H p65 gene expression analysis by qPCR (2−ΔΔCt) and I quantification of H3K4me1, H3K9me3 modifications on the promoter by ChIP assay. The data are expressed as FC (CAD vs CAD-T2DM; unpaired t-test)

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Cardiovascular Diabetology

ISSN: 1475-2840