Fig. 5

Phenotypical characterization of HG-CD34⁺- derived monocytes. A analysis of the total monocytes (CD14⁺) generated from HG-CD34⁺ cells after myeloid differentiation and (B) of their intermediate (CD14^hiCD16⁺) and (C ) classical (CD14^hiCD16) subpopulations by flow cytometry. The data are expressed as FC (NG vs HG; paired t-test). D Flow cytometric dot blot analysis of a representative differentiation experiment. E through G, p21 and p27 gene expression analysis by qPCR (2^−ΔΔCt) and WB. The data are expressed as FC (NG vs HG; paired t-test). H quantification of proinflammatory cytokines released in the medium after LPS stimulation. The data are expressed as FC (NG vs HG; paired t-test). I p65 gene expression analysis by qPCR (2^−ΔΔCt) and (L) quantification of H3K4me1, H3K9me3 modifications on its promoter by ChIP assay. The data are expressed as FC (NG vs HG; paired t-test)