Fig. 4

HG exposure modulates the activation of NFkB-p65 transcription factor by epigenetic mechanism. A qPCR quantification of KAT3B gene; the data are represented as FC over NG (2-^ΔΔCt; NG vs HG; paired t-test). B Analysis of post-translational modification at lysine 310 (K310) by WB of p65 in HG and exHG-CD34⁺ cells. Blot image is representative of at least 3 independent experiments. C Nuclear compartmentalization analysis of NFkB-p65 in HG and exHG-CD34⁺ cells after DRAQ5™ and NFkB-p65 antibody staining by Amnis ImageStream^®X Mk II. The merge fluorescence of DRAQ5/NFkB-p65 is expressed as Similarly Dilate Index. D Binding activity of NFkB-p65 in NG, HG, and exHG-CD34⁺ cells. Data are expressed as percentage of NG (1-way ANOVA). KAT3B, Lysine acetyltransferases (KATs), p300