Fig. 3

NFkB-p65 upregulation correlates with a more open chromatin conformation in HG-CD34 + cells. A evaluation of NFkB-p65 expression in NG-, HG-, and exHG-CD34 + cells by qPCR and WB analysis. Blot image is representative of at least 3 independent experiments. qPCR data are expressed as FC over NG (2^−ΔΔCt; 1-way ANOVA). B qPCR quantification of SETD7 gene; the data are represented as FC over NG (2^−ΔΔCt; 1-way ANOVA). C through F, ChIP of the p65 promoter by SETD7, H3K4me1, H3K9me3 and RNA POL II antibody in NG-,HG-, and exHG-CD34⁺ cells. The data, after input normalization, are expressed as a percentage of NG (1-way ANOVA). ChIP indicates chromatin immunoprecipitation; SETD7, SET (Su(var)3–9, Enhancer-of-zeste and Trithorax) domain containing lysine methyltransferase 7; H3K4me1, aminomethylation on lysine 4 of histone H3; H3K9me3, trimethylation on lysine 9 of histone H3; RNA POL II, RNA polymerase II