Fig. 2

Effect of HG on CD34 + HSPC phenotype. A Proliferation curve of CD34⁺ cells exposed to HG condition (30 mmol/L; n = 5; ***P < 0.001; 2-way ANOVA). B and C flow cytometric quantification of ROS production and AnnexinV staining in HG-treated cells respectively. Data are reported as Fold-Change (FC) of Mean fluorescence intensity (MFI) over NG (B) (1-way ANOVA) and % of positive cells (C). D qPCR quantification of telomere length, data are expressed as FC (2^−ΔΔCt; NG vs HG; paired t-test). E through H p21, p27, IL6 and TNFα expression analysis by qPCR and (G) Western blot (WB). Blot image is representative of 3 independent experiments. Data are represented as FC over NG (2^−ΔΔCt for qPCR; 1-way ANOVA). I secretion levels in culture media of IL6 and TNFα from HG and NG-CD34⁺ cells. Data are represented as FC over NG (1-way ANOVA)