Fig. 5

Irisin inhibits HG-induced cell damage and ferroptosis in H9C2 cells. H9C2 cells were incubated with HG (35 mmol/L) for 24 h in the presence or absence of irisin (10–40 nmol/L) before physiological/biochemical assessment. The cell viability of H9C2 cells was determined by the CCK-8 Kit (A) and LDH release assay (B) (n = 6 per group). MDA (C), GSH (D), and GSH/GSSG ratio (E) in H9C2 cells were determined using the relevant kits (n = 6 per group). F Western blots of the proteins SLC7A11 and GPX4 in H9C2 cells. Representative blots from a mouse in each of the four groups of mice (F1) and quantitative analysis of SLC7A11 and GPX4 (F2) are shown (n = 4 per group). GAPDH served as the loading control. G Representative images of fluorescence probe for ROS from H9C2 cells in each of the four groups of H9C2 cells (G1) with quantification (G2) (Scale bar = 50 μm; n = 6 per group). H1 Representative fluorescent images of FerroOrange staining in H9C2 cells in each of the four groups of H9C2 cells (Scale bar = 50 μm). H2 The quantitative results of FerroOrange staining are shown (n = 6 per group). Data are presented as the mean ± SD.One-way ANOVA, and Bonferroni’s post-hoc test. ^*P < 0.05, ^**P < 0.01. HG high glucose, MDA malondialdehyde, GSH reduced glutathione, GSSG oxidized glutathione, SLC7A11 solute carrier family 7 member 11, and GPX4 glutathione peroxidase 4, and LDH lactate dehydrogenase