Fig. 3

Irisin attenuates ferroptosis in the heart of type 1 diabetic mice. A MDA, B LPO, C GSH, and D GSH/GSSH ratio in cardiac tissue lysates were measured from the four groups of mice (n = 6 per group). E Representative images (E1) and the statistical results (E2) of the iron staining (Scale bars = 20 μm; n = 6 per group). F Representative Western blots (F1) and quantification (F2) showing myocardial protein levels of SLC7A11 and GPX4 in the four groups of mice (n = 6 per group). G Representative TEM images (G1) showing the mitochondria of cardiac tissue from a mouse in each of the four groups of mice (Scale bars = 500 nm) and (G2) The corresponding relative Flameng scores were shown (n = 6 per group). Data are expressed as the mean ± SD.One-way ANOVA, and Bonferroni’s post-hoc test. *P < 0.05, **P < 0.01. STZ streptozotocin, MDA malondialdehyde, LPO lipid peroxides, GSH reduced glutathione, GSSG oxidized glutathione, TEM transmission electron microscopy, SLC7A11 solute carrier family 7 member 11, and GPX4 glutathione peroxidase 4