Fig. 1

The heart tissue and serum levels of irisin are decreased in type 1 diabetic mice. A A schematic diagram showing the treatments of the mice. B Western blot of serum samples obtained from type 1 diabetic mice and control non-diabetic mice (10 μL serum per lane). Ponceau S staining Albumin served as loading control (n = 8 per group). C Irisin protein expression in the heart was determined by Western blot. GAPDH served as the loading control. Data are expressed as the mean ± SD. Student’s t-test.^*P < 0.05, ^**P < 0.01. STZ streptozotocin