Fig. 4
A Spearman correlation analysis between MCT4 and CD68 mRNA expression in the heart of mice. B Representative diagram of CD68 expression around the high expression of MCT4 in the heart of m Leprdb and Leprdb mice. C A diagram to depict the coculture experiment using transwell. RAW264.7 and H9C2 (pre-treated with PA and/or VB124) were cocultured for 24 h for D–F. D Representative diagram and quantification of migrated RAW264.7 cells stained with crystal violet. E mRNA expression of iNOS and Hif-1α in coculture RAW264.7. F Representative diagram and quantification of Pan Kla% in coculture RAW264.7. G Flow cytometry analysis of RAW264.7 macrophage subtypes treated with either PA, lactic acid (LAC), or a combination of both. H Representative diagram and quantification of H3K18La and H4K12La in RAW264.7 treated with PA and/or LAC. I mRNA expression of Tnf-α and Il-1β in RAW264.7 treated with PA and/or LAC. J Hif-1α mRNA expression in RAW264.7 treated with PA and/or LAC. K Chromatin IP (ChIP) quantitative real time PCR (ChIP-qRT-PCR) of gene targets for HIF-1α and iNOS at H4K12La in RAW264.7 treated with PA or PA + LAC