Skip to main content
Fig. 1 | Cardiovascular Diabetology

Fig. 1

From: Heparanase inhibition as a systemic approach to protect the endothelial glycocalyx and prevent microvascular complications in diabetes

Fig. 1

HS forms part of the eGlx barrier in retinal and glomerular microvasculature. a Left: low magnification representative TEM image of retinal vessel from mouse that had been cardiac perfused with Alcian blue and glutaraldehyde. Lumen (Lm), retinal endothelial cell (REC), and pericyte (Pr) indicated. Right: high magnification image showing eGlx staining on luminal side of REC (arrow heads). Basement membrane (BM) indicated. REC vesicles (*) were also visible. b Retinal flat mounts stained with anti-HS in green. DAPI in blue. Retinal endothelial cell (REC) and lumen (Lm) indicated. Note the green signal on luminal side of REC nucleus (arrowhead), indicating presence of HS in the eGlx. c Left: low magnification representative EM image of glomerular capillary from mouse that had been cardiac perfused with Alcian blue and glutaraldehyde. Lumen (Lm) and glomerular filtration barrier (GFB) indicated. Right panel: High magnification image showing eGlx staining (arrow heads) on luminal glomerular endothelial cell (GEnC). Glomerular basement membrane (GBM) and podocyte (P) indicated. d Mouse kidney tissue stained with anti-HS in green, DAPI in blue, membranes in red (R18). Inset shows zoomed in image of eGlx HS staining (white arrow head) on luminal (LM) side of vessel. *GBM.

Back to article page

Cardiovascular Diabetology

ISSN: 1475-2840