Fig. 3

Protection of AA against high glucose (HG) + oxygen deprivation (OGD) induced myocardial cell death. H9C2 cells were grouped into high glucose (HG, 33 mM), OGD, or HG + OGD. To establish the OGD cell model, cell culture medium was replaced with Tyrode’s solution without glucose and the cell plate was moved into a controlled hypoxic chamber for 3 h. Then, intracellular AA concentration was detected with or without OGD + HG treatment, and MTT and TUNEL methods were used to determine the change of cell viability and apoptosis before and after AA treatment. A intracellular AA level; B the time-course changes in AA levels in HG + OGD treated H9C2. C The MTT assay was used to determine the cell viability. D TUNEL method was used to detect apoptosis induced by HG + OGD. E, F, effect of AA on HG + OGD treated H9C2 cells viability by MTT assay and cell apoptosis by the TUNEL assay. G Representative images of TUNEL assay, the blue spots represent cell nuclei (DAPI staining) and the green spots represent apoptotic bodies. The results are expressed as the mean ± standard deviation of data from 3 independent experiments; **P < 0.01, ***P < 0.001. Statistical analysis was carried out by a one-way ANOVA analysis. a.u. = arbitrary units