Fig. 2

Metabolites (up- and down-regulated) and related pathways modulated by DM in pigs with MI identified by metabolomic profiling of coronary sinus blood. Half hour after myocardial infarction, blood was collected from the coronary sinus for metabonomic analysis. A Representative base peak intensity (BPI) chromatograms from Bama- minipig plasma samples. The box indicates the BPI with a retention time of about 24 min, at which time AA is identified. B Two dimensional scores plot of a PLS-DA with orthogonal signal correction data filter of the first and second PLS component of plasma. The solid square represented sham group, blank squares represented MI group, and the solid triangles represented DM + MI group. C The map of differentially regulated metabolites; D pathway enrichment analysis identified AA metabolic pathway enriched most significantly. The calculated P-value was gone through FDR Correction, taking FDR ≤ 0.05 as a threshold. n = 10 in each group. E Determination of the concentration of PLA2; F schematic diagram of PLA2-catalyzed dissociation of AA from cell membranes in the context of acute myocardial ischemia in diabetes. P-values were obtained using an unpaired t-test. ***P < 0.001. Statistical analysis was carried out by a one-way ANOVA analysis. AA, arachidonic acid; MI: acute myocardial infarction; BPI: base peak intensity; DM: diabetes mellitus; PLS-DA: partial least squares discriminant analysis. PLA2: phospholipase A2