Fig.1

DM increased infarct size and impaired heart function in pigs with MI. Bama-mini pigs were first subjected to intravenous injection of STZ (150 mg/kg) to induce DM disease, and then underwent coronary artery ligation surgery to establish an acute myocardial infarction model. Hematology, coronary angiography, electrocardiogram, echocardiography, MRI, and TTC staining were used to evaluate the animals. SEM was used to detect the ultrastructural changes in cardiomyocytes in the peripheral area of infarction. A Study design. B FBGs of diabetic pigs post STZ administration; C Representative coronary angiography images of sham group (left), MI group (middle) and DM + MI group (right). The arrows show the coronary ligation site. D Representative images of electrocardiogram immediately after surgery. The arrows show the elevated S–T segment. E, Representative echocardiographs and F ejection fraction of mini-pigs was determined to evaluate cardiac function at 48 h after surgery. G Serum cTnI concentration was compared at 2 h after surgery. H Representative cardia MRI image from 10 animals. Red circle indicates the infarct area. I Representative images of TTC stained myocardium of mini-pigs. Red arrows indicated infarcted myocardia. J Quantification of infarct size. n = 10–12; K Representative images of SEM; L quantification of intact mitochondria. M analysis of the percentage of mitophagosomes. P-values were obtained using an unpaired t-test. *P < 0.05, ***P < 0.001. Statistical analysis was carried out by a one-way ANOVA analysis. DM, diabetes mellitus; MI, acute myocardial infarction; cTnI, cardiac troponin I; FBG, fasting blood glucose; LAD, left anterior descending coronary artery; MRI, magnetic resonance imaging; TTC,2,3,5-triphenyltetrazolium chloride; SEM, scanning electron microscopy