Fig. 4
From: ASGR1 deficiency diverts lipids toward adipose tissue but results in liver damage during obesity
ASGR1 deficiency activates the immune cell surveillance in VAT. VAT immunophenotyping in two experimental groups (n = 8–12), after 20 weeks of HFD. A A representative flow cytometry gating strategy for the natural killer cells (NK cells from CD45+) and NK subsets (NKreg and NKcito), monocytes and neutrophils. B–G Immune cells quantification in WT and ASGR1−/− VAT after 20 weeks of HFD expressed as cells per gram of tissue. B Count in monocytes (GR-1+), C macrophages (F4/80+), D neutrophils (SSC-HhighGR-1+), E natural killer cells (NK, NK1+), F dendritic cells (DC, CD 11c+) and G T cells (CD19− CD3+) corrected for the VAT weight (gr.). Each bar and error represent the mean ± SEM. *p < 0.05, **p < 0.01 and ***p < 0.001