Fig. 7

Mel attenuated DCM through VEGF-B/PERK signaling pathway. A Representative images of echocardiography in mice. B Values of EF and (C) FS, n = 6. D CK-MB release in serum, n = 6. E Myocardial tissues were stained with H&E (scale bar = 50 μm), WGA (scale bar = 50 μm), Masson (scale bar = 100 μm) and Sirius red (scale bar = 50 μm). F Size of myocardial cells were assessed by staining with WGA, n = 8. G Ratio of heart weight to tibia length, n = 6. H The mRNA expression of Myhc, n = 6. I Western blot for PERK-related pathways and autophagy proteins in GSK treated mice, n = 3. J Western blot for PERK-related pathways and autophagy proteins in mice, n = 3. K Immunofluorescence staining for ATF4 translocation in NRVMs (scale bar = 10 μm). L Autophagic flux in NRVMs (scale bar = 10 μm). Data were expressed as the mean ± SD.*p < 0.05, **p < 0.01, ***p < 0.01, ****p < 0.0001. One-way ANOVA followed by a post hoc Tukey’s test