Fig. 6

Mel enhanced autophagy and protected against DCM via increasing phosphorylation of PERK. A Co-immunoprecipitation was performed to detect the interaction of PERK and GRP78 in NRVMs. B The experimental protocol in mice. C Representative images of echocardiography in mice. D Values of EF and (E) FS, n = 6. F CK-MB release in serum, n = 6. G Ratio of heart weight to tibia length, n = 6. H, I The mRNA expressions of Myhc and Anf, n = 6. J Myocardial tissues were stained with H&E (scale bar = 50 μm), WGA (scale bar = 50 μm), Masson (scale bar = 100 μm) and Sirius red (scale bar = 50 μm). K Size of myocardial cells were assessed by staining with WGA, n = 8. L Western blot for PERK-related pathways and autophagy proteins mice, n = 3. M Autophagic flux in NRVMs (scale bar = 10 µm) Data were expressed as the mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.01, ****p < 0.0001. One-way ANOVA followed by a post hoc Tukey’s test