Fig. 5

VEGF-B interacted with GRP78 in NRVMs. A Co-immunoprecipitation was performed to detect the interaction of VEGF-B and GRP78 in NRVMs and (B) in 293 T cells. C We constructed GST-GRP78-FL plasmid, expressed and purified in Rosetta (DE3). D Co-incubated with Flag-VEGF-B to detect the interaction between GRP78 and VEGF-B. E Western blot for GRP78 and quantification for GRP78 in mice, n = 3. F Western blot for GRP78 and quantification for GRP78 in NRVMs, n = 3. G Western blot for GRP78 and VEGF-B in NRVMs. H Quantification for GRP78 and VEGF-B in NRVMs, n = 3. I Immunofluorescence staining for VEGF-B, GRP78, and their co-localization in NRVMs (scale bar = 50 μm). J Different truncates of GRP78. K We established and purified GST-GRP78-FL, GST-GRP78-NBD and GST-GRP78-SBD in Rosetta (DE3). L VEGF-B interacted with SBD domain, NBD domain and FL of GRP78. Data were expressed as the mean ± SD.*p < 0.05, **p < 0.01. One-way ANOVA followed by a post hoc Tukey’s test