Fig. 3

Mel down-regulated expression of VEGF-B either in vivo or in vitro. A The mRNA expression of Vegfb in mice, n = 6. B Western blot for VEGF-B in mice. C Quantification for VEGF-B in mice, n = 3. D The immunohistochemical analysis for VEGF-B in mice myocardial tissues (scale bar = 100 μm). E The mRNA expression of Vegfb in NRVMs, n = 5. F Western blot for VEGF-B in NRVMs. G Quantification for VEGF-B in NRVMs, n = 3. H The NRVMs were subjected to immunofluorescence analysis with anti-VEGF-B antibody (scale bar = 100 μm). I Quantification for mean fluorescence of VEGF-B in NRVMs, n = 5. Data were expressed as the mean ± SD.*p < 0.05, **p < 0.01, ***p < 0.001. One-way ANOVA followed by a post hoc Tukey’s test