Fig. 2

Mel attenuated DCM through increasing cardiomyocytes autophagy. A Western blot for p62 and LC3 in mice. B Quantification for p62 and LC3-II/LC3-I in mice, n = 3. C Western blot for p62 and LC3 in NRVMs. D Quantification for p62 and LC3-II/LC3-I in NRVMs, n = 3. E Autophagic flux was used to detect autophagy in NRVMs (scale bar = 10 µm). F Western blot for p62 and LC3 in NRVMs. G Quantification for p62 and LC3-II/LC3-I in NRVMs, n = 3. H Autophagic flux in NRVMs (scale bar = 10 µM) I Calcein-AM/PI double staining in NRVMs (scale bar = 200 μm) J Quantification of PI-positive cells, n = 5. Data were expressed as the mean ± SD.*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. One-way ANOVA followed by a post hoc Tukey’s test