Fig. 1

Mel alleviated cardiac dysfunction in diabetic mice. A Representative images of echocardiography in mice. B, C Values of EF and FS, n = 6. D CK-MB release in serum, n = 6. E Ratio of heart weight to tibia length, n = 6. F Myocardial tissues were stained with H&E (scale bar = 50 μm), WGA (scale bar = 50 μm), Masson (scale bar = 100 μm) and Sirius red (scale bar = 50 μm). G Size of myocardial cells were assessed by staining with WGA, n = 8. H, I The mRNA expressions of Anf and Myhc, n = 6. J Calcein-AM/PI double staining in NRVMs (scale bar = 200 μm). K Quantification of PI-positive cells, n = 5. Data were expressed as the mean ± SD.*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. One-way ANOVA followed by a post hoc Tukey’s test