Fig. 9

DAPA targets SIRT1 to deacetylate NICD and promote its proteolysis. A Immunoblot analysis of total acetylation level. B PPI networks of SIRT1. C NICD acetylation level was measured by IP using an anti-acetylation antibody and Western blotting. D The effects of MG132 on NICD protein expression upon DAPA. E Western blotting analysis of NICD expression levels in the nucleus and cytoplasm of HUVECs and MAECs treated as indicated and quantified. F Immunofluorescent staining of SIRT1 in HUVECs and MAECs treated as indicated. Scale bars = 50 μm. *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001 vs. control group; ^#p < 0.05, ^##p < 0.01, ^###p < 0.001 and ^####p < 0.0001 vs. the TGF-β1 group