Fig. 8

DAPA inhibits EndMT by regulating the Notch1 signaling pathway. A SIRT1 is implicated in the regulation of KEGG signaling pathways (Show only the top ten). B Representative Western blotting and quantitative analysis of NICD in HUVECs and MAECs treated with TGF-β1 for 48 h. C Representative Western blotting and quantitative analysis of NICD in HUVECs and MAECs treated with different concentrations of DAPA for 48h in the presence of TGF-β1. D Representative Western blotting showing NICD expression in vivo treated as indicated and quantified. E The binding conformation of SIRT1 and activated Notch1. The left image is the cartoon, the right image is the surface, and the yellow area is the binding region. F Co-IP of NICD and SIRT1. *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001 vs. control group; #p < 0.05, ##p < 0.01 vs. the TGF-β1 group