Fig. 7

DAPA promotes nucleus translocation of SIRT1. A The overall binding view and local binding view of the SIRT1-DAPA complex obtained based on docking; the yellow dotted line represents the hydrogen bond, the green line represents the amino acid forming a hydrogen bond with the small molecule DAPA in the protein binding pocket, the cartoon represents the SIRT1 protein, and the purple stick is DAPA. B Chemical structure of DAPA and biotin-labeled DAPA (Bio-DAPA). The eluted product was obtained using a biotinylated protein interaction pull-down kit and further analyzed by Western blotting experiments. Total lysates were used as an input control. C CETSA between DAPA and SIRT1. The curve was fitted using GraphPad Prism 9.5.1. D Nucleus-cytoplasm distribution of SIRT1 in cells treated with TGF-β1and DAPA. E Immunofluorescent staining of SIRT1 in HUVECs and MAECs treated as indicated. Scale bars = 50 μm. *p < 0.05, **p < 0.01, and ***p < 0.001 vs. control group; #p < 0.05, and ##p < 0.01vs. the TGF-β1 group