Fig. 1

DAPA ameliorates ISO-induced myocardial perivascular fibrosis and cardiac dysfunction by targeting endothelial cells. A Images of transthoracic echocardiograms in M-mode (n = 6). B, H&E staining of the lengthwise section of the heart. Scale bar = 100 μm. C Representative images showing Masson staining of perivascular fibrosis in heart sections. Scale bar = 100 μm. D Representative images of perivascular fibrosis stained with PSR, and red represents collagen fiber deposition. Scale bars = 100 μm. E tSNE plots and UMAP plots. Each point depicts a single cell, colored according to cluster designation. F The proportion of the corresponding cell types. G Cell proportion of 2 sample sources in each cell type. H Rose diagrams showing the differentially expressed genes (DEGs) numbers after HF in each cell type. I Top 10 of GO terms and pathways enriched in DEGs of HF and Normal. J Circle plot showing the total intercellular signaling network in the HF group. Blue indicates weakening, while red indicates strengthening after HF. ^*p < 0.05, ^**p < 0.01, ^***p < 0.001 and ^****p < 0.0001 vs. Vehicle group; ^#p < 0.05, ^##p < 0.01, ^###p < 0.001 and ^####p < 0.0001 vs. the ISO group