Fig. 5
From: Direct regulation of the cardiac ryanodine receptor (RyR2) by O-GlcNAcylation
S2808 is required for CaMKII independent O-GlcNAcylation mediated changes in SOICR. (A) Representative blots showing O-GlcNAc-RyR2 and total RyR2. (B) Densitometric analysis of O-GlcNAc-RyR2 level. All O-GlcNAc-RyR2 was normalised to total RyR2. Results are shown as Mean ± SEM. n = 4 independent lysates. Statistical analysis was performed using an unpaired t-test. (C) Representative CEPIA traces for S2808 cells without Thm-G (A) and with Thm-G treatment (D). Comparison of (E) release threshold, and (F) fractional release in S2808 cells treated with or without Thm-G. Results were obtained from 125 and 135 cells (for – and + Thm-G, respectively) recorded over 8 independent experiments. To remove the impact of CaMKII, KN-93 was present in all conditions. Results are expressed as Mean ± SEM. Statistical analysis was performed using a Mann-Whitney test