Fig. 2
From: Direct regulation of the cardiac ryanodine receptor (RyR2) by O-GlcNAcylation
Changes in RyR2 O-GlcNAcylation are associated with changes in SOICR. Application of Thm-G and DON on the O-GlcNAc-RyR2 level in cells grown in low glucose (5.5 mM) and high glucose (25 mM) media. (A) Representative blots showing O-GlcNAc-RyR2 and total RyR2. (B) Densitometric analysis of O-GlcNAc-RyR2 level. All O-GlcNAc-RyR2 was normalised to total RyR2. Results are shown as Mean ± SEM. n = 3 independent lysates. Statistical analysis was performed using two-way ANOVA (Šidák post-hoc test). (C) Representative Fluo-4 traces presented as F/F0. Peaks represent SOICR events. (D) Percentage of cells experiencing SOICR at 5.5 mM glucose, (E) percentage of cells experiencing SOICR at 25 mM glucose. Data displayed are the average of 5–8 independent experiments and expressed as Mean ± SEM. Only significant P-values, vs. control are shown. Statistical analysis was performed using two-way ANOVA (Tukey post-hoc test)