Skip to main content
Fig. 5 | Cardiovascular Diabetology

Fig. 5

From: Evidence for a lack of inotropic and chronotropic effects of glucagon and glucagon receptors in the human heart

Fig. 5

A Western blot analysis showing the absence of glucagon receptor expression in samples of sinoatrial node (SN), right atrium (RA), left atrium (LA), right ventricle (RV) and left ventricle (LV) from a failing (F) and a nonfailing (NF) human heart. Samples correspond to heart donors number 5 F and number 6 (NF) in Table 1. α-Tubulin was used as the loading control. Human liver (HL) was used as the positive control. Band intensity was determined with densitometry using the program Image Quant TL Plus (General Electrics, USA). B The absence of glucagon receptor mRNA in failing (n = 5) and nonfailing (n = 5) human myocardium samples. Samples were obtained from the sinoatrial node (SN), right atrium (RA), left atrium (LA), right ventricle (RV), and left ventricle (LV). Human liver (HL) tissue was used as the positive control. Each bar represents the combined result of samples obtained from failing and nonfailing hearts (n = 10). Only one sample of the human liver was used as the control. *P < 0.015 compared to HL (parametric binomial analysis)

Back to article page

Cardiovascular Diabetology

ISSN: 1475-2840