Fig. 9

Collagen levels in the heart affected by the combination of diabetes and Hif1a mutation. A Representative images of picrosirius red staining (PSR) of collagen in the LV and B delineated collagen⁺ area in the LV myocardium using Adobe Photoshop. Scale bar = 50 µm. C PSR⁺ area was quantified using ImageJ and expressed as a percentage of the area of the LV. Data are mean ± SD (n = 5 hearts per genotype and one section per sample). D, E Representative Western blots and quantification of collagen type I (COL1) in the whole heart. Data are mean ± SD (n = 5). Statistical significance assessed by two-way ANOVA followed post hoc Tukey ‘s multiple comparisons test, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. LV, left ventricle; AU, arbitrary units