Fig. 2

Hif1a-mediated transcription signature in sympathetic neurons. A Workflow depicts microdissection, dissociation, FACS sorting of single tdTomato⁺ sympathetic neurons for a bulk of 100 cells-RNAseq analysis, and qRT-PCR validation from RNA isolated from the microdissected sympathetic chain from 4-month-old Hif1aCKO-Ai14 (n = 5) and control-Ai14 mice (n = 3). B The volcano plot shows the change in protein-coding gene expression levels in the Hif1aCKO-Ai14 compared to control-Ai14 sympathetic neurons (adjusted p-value < 0.01, and fold change > 1.5 cutoff values). The complete list of identified down- and up-differentially expressed genes is in Additional file 10: Dataset S1. C Enrichment map of down- and upregulated gene ontology (GO) sets visualized by the network. Each node represents a GO term; edges depict shared genes between nodes. Each GO set cluster was assigned with representative keywords; a list of GO sets is available in Additional file 10: Dataset S2. D The most enriched functional categories for down- and up-regulated genes identified by RNAseq. E Validation of relative mRNA expression levels of selected genes by qRT-PCR. mRNA was isolated from the microdissected secondary sympathetic chain of control-Ai14 (n = 3) and Hif1aCKO-Ai14 mice (n = 4). Statistical significance assessed by unpaired t test: genotype effect (*P < 0.05). Brinp2, BMP/retinoic acid inducible neural specific 2; Dcn, decorin; Epha5, Eph receptor A5; Pros1, protein S (alpha); Tet3, tet methylcytosine dioxygenase 3; Tlr4, toll-like receptor 4